Izinhlobo ezingama-28 ze-high dapilloma virus virus ephezulu (16/18 typing) nucleic acid
Igama lomkhiqizo
I-HWTS-CC006A-28 Izinhlobo ze-Hish-Suman Papilloma Virus (16/18 Ukuthayipha) I-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Umdlavuza wesibeletho ungenye yezimpawu ezivame kakhulu zobuhlanya zepheshana lokuzala labesifazane. Ucwaningo lukhombisile ukuthi izifo eziphikelelayo ze-HPV nokutheleleka okuningi kungenye yezimbangela eziphambili zomdlavuza wesibeletho. Njengamanje, ukwelashwa okusebenzayo okuqashelwayo kusantuleka ngomdlavuza wesibeletho obangelwa i-HPV, ukutholwa kusenesikhathi nokuvimbela ukufunwa komlomo wesibeletho okubangelwa yi-HPV kuyisihluthulelo sokuvimbela umdlavuza wesibeletho. Kubaluleke kakhulu ukusungula isivivinyo esilula, esikhethekile nesisheshayo sokuhlolwa kwe-Etiology sokuxilongwa komtholampilo nokwelashwa komdlavuza womlomo wesibeletho.
Insungubezi
| Ukuhlanganiswa reaction | Insungubezi | Ukuthayipha |
| PCR-MIX1 | -Yenza | 18 |
| I-VIC (Hex) | 16 | |
| Ivupho | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68, 68 | |
| Cy5 | Ukulawulwa Kwangaphakathi | |
| PCR-MIX2 | -Yenza | 6, 11, 54, 83 |
| I-VIC (Hex) | 26, 44, 61, 81 | |
| Ivupho | 40, 42, 43, 53, 73, 82 | |
| Cy5 | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | Uketshezi: ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | I-Cervical Swab, i-vaginal swab, umchamo |
| Ct | ≤28 |
| CV | <5.0% |
| Indlu edayisa insimbi | 300copies / ml |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-reaglent enconyiwe ye-realent: I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3017-50, HWTS-3017-50, HWS-3017-48, HWS-3017-48, HWS-3017-46, HWS-3017-46, I-HWS-3017-96, (engasetshenziswa ngeMacro & Micro-Test I-automatic nucleic acid extractor (HWTS-3006C, HWS-3006B)) nguJiangsu I-Macro & Micro-Test Med-Tech Co, Ltd. engeza i-200μL ye-saline ejwayelekile yokuvuselela i-pellet ku-STEP 2.1, bese kuthi ukukhishwa kufanele kwenziwe ngokuya ngemiyalo yokusebenzisa le mpendulo ye-extraction. IVolumu Ye-Expeated Enconyiwe ingu-80μL.
I-realent enconyiwe ye-realent: Qiamp DNA Mini Kit (51304) noma i-Macro & Micro-Test Viral DNA / RNALM COLLM (HWTS-3020-50). Faka i-200μL ye-saline ejwayelekile yokuvuselela i-pellet in Step 2.1, bese kuthi ukukhishwa kufanele kwenziwe ngokuya ngemiyalo yokusebenzisa le mpendulo ye-extraction. Ivolumu yesampula ekhishwe amasampula yonke i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-100μL.
