Izinhlobo ezingu-28 ze-Human Papilloma Virus eyingozi kakhulu (uhlobo lwe-16/18) i-Nucleic Acid

Incazelo emfushane:

Le khithi ifanelekela ukutholwa kwezinhlobo ezingu-28 zamagciwane e-human papilloma (HPV) (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) ze-nucleic acid kumchamo wesilisa/wesifazane kanye namaseli akhishwe esibelethweni sowesifazane. I-HPV 16/18 ingabhalwa nge-in vitro, izinhlobo ezisele azikwazi ukubhalwa nge-in vitro, okuhlinzeka ngemithi yokusiza yokuxilongwa nokwelashwa kokutheleleka kwe-HPV.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Izinhlobo ze-HWTS-CC006A-28 ze-Human Papilloma Virus eyingozi kakhulu (16/18 Typing) I-Nucleic Acid Detection Kit (i-Fluorescence PCR)

I-Epidemiology

Umdlavuza womlomo wesibeletho ungenye yezimila eziyingozi ezivame kakhulu ohlelweni lokuzala lwabesifazane. Izifundo zikhombisile ukuthi ukutheleleka okuqhubekayo kwe-HPV kanye nokutheleleka okuningi kungenye yezimbangela eziyinhloko zomdlavuza womlomo wesibeletho. Njengamanje, ukwelashwa okusebenzayo okuqashelwayo kusantuleka komdlavuza womlomo wesibeletho obangelwa yi-HPV, ngakho-ke ukutholakala kusenesikhathi nokuvimbela ukutheleleka komlomo wesibeletho okubangelwa yi-HPV kuyisihluthulelo sokuvimbela umdlavuza womlomo wesibeletho. Kubaluleke kakhulu ukusungula isivivinyo sokuxilonga esilula, esiqondile nesisheshayo se-etiology sokuxilongwa kanye nokwelashwa komdlavuza womlomo wesibeletho.

Isiteshi

Ingxube Yokusabela	Isiteshi	Uhlobo
I-PCR-Mix1	I-FAM	18
	I-VIC(HEX)	16
	I-ROX	31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68
	CY5	Ukulawula Kwangaphakathi
I-PCR-Mix2	I-FAM	6, 11, 54, 83
	I-VIC(HEX)	26, 44, 61, 81
	I-ROX	40, 42, 43, 53, 73, 82
	CY5	Ukulawula Kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	Uketshezi: ≤-18℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	I-Swab Yesibeletho, I-Swab Yesitho Sangasese Sowesifazane, Umchamo
Ct	≤28
CV	<5.0%
I-LoD	Amakhophi angu-300/mL
Izinsimbi Ezisebenzayo	Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio^®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler^®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR

Ukugeleza Komsebenzi

I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Engeza u-200μL we-saline evamile ukuze uphinde umise i-pellet esinyathelweni 2.1, bese ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhipha. Ivolumu yokususa enconyiwe ingu-80μL.
I-reagent yokukhipha enconyiwe: I-QIAamp DNA Mini Kit (51304) noma i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Engeza u-200μL we-saline evamile ukuze uphinde umise i-pellet esinyathelweni 2.1, bese ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhipha. Ivolumu yesampula ekhishwe yamasampula ingu-200μL, kanti ivolumu yokususa enconyiwe ingu-100μL.

Okwedlule: I-Enterococcus engamelani ne-Vancomycin kanye ne-Gene engamelani nemithi

Olandelayo: I-Human Papillomavirus (Izinhlobo ezingu-28) Ukuhlolwa kwezakhi zofuzo

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