I-CYP2C19 Gene Polymorphism yomuntu

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwe-in vitro qualitative ye-polymorphism ye-CYP2C19 yofuzo CYP2C19*2 (rs4244285, c.681G>A), CYP2C19*3 (rs4986893, c.636G>A), CYP2C19*20 c1485 (rs168) >T) ku-DNA ye-genomic yamasampula egazi eliphelele lomuntu.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-GE012A-Human CYP2C19 Gene Polymorphism Detection Kit (Fluorescence PCR)

Isitifiketi

CE/TFDA

I-Epidemiology

I-CYP2C19 ingenye yezidakamizwa ezibalulekile ezihlanganisa ama-enzymes emndenini we-CYP450.Ama-substrates amaningi e-endogenous kanye cishe no-2% wemithi yomtholampilo agaywa yi-CYP2C19, njenge-metabolism ye-antiplatelet aggregation inhibitors (njenge-clopidogrel), i-proton pump inhibitors (omeprazole), ama-anticonvulsants, njll. I-CYP2C19 gene polymorphisms nayo inomehluko ku-metabolism izidakamizwa ezihlobene.Lezi zinguquko zamaphuzu ze-*2 (rs4244285) kanye *3 (rs4986893) zibangela ukulahleka komsebenzi we-enzyme ofakwe yisakhi sofuzo se-CYP2C19 kanye nobuthakathaka bekhono le-metabolic substrate, futhi kwandise ukugcwala kwegazi, ukuze kubangele ukusabela okubi kwezidakamizwa okuhlobene ukugxila kwegazi.* I-17 (rs12248560) ingakhuphula umsebenzi we-enzyme efakwe yisakhi sofuzo se-CYP2C19, ukukhiqizwa kwama-metabolites asebenzayo, futhi ithuthukise ukuvinjelwa kokuhlangana kweplatelet futhi yandise ingozi yokopha.Kubantu abane-metabolism ehamba kancane yezidakamizwa, ukuthatha imithamo evamile isikhathi eside kuzodala ubuthi obukhulu kanye nemiphumela emibi: ikakhulukazi ukulimala kwesibindi, ukulimala kwesistimu ye-hematopoietic, ukulimala kwesistimu yezinzwa ezimaphakathi, njll., okungaholela ekufeni ezimweni ezimbi kakhulu.Ngokomehluko ngamunye kumetabolism yezidakamizwa ehambisanayo, imvamisa ihlukaniswe yaba ama-phenotypes amane, okuyi-ultra-fast metabolism (UM, *17/*17,*1/*17), i-fast metabolism (RM,*1/*1). ), i-metabolism ephakathi (IM, *1/*2, *1/*3), i-slow metabolism (PM, *2/*2, *2/*3, *3/*3).

Isiteshi

FAM I-CYP2C19*2
CY5 I-CYP2C9*3
I-ROX I-CYP2C19*17
VIC/HEX IC

Imingcele Yezobuchwepheshe

Isitoreji Uketshezi: ≤-18℃
Impilo yeshelufu Izinyanga ezingu-12
Uhlobo lwesifanekiso Igazi elisha le-EDTA elingenayo i-anticoagulated
CV ≤5.0%
LoD 1.0ng/μL
Ukucaciswa Akukho ukusebenza kabusha okuphambene nokunye ukulandelana okungaguquki kakhulu (i-CYP2C9 gene) ku-genome yomuntu.Ukuguqulwa kwamasayithi e-CYP2C19*23, CYP2C19*24 kanye ne-CYP2C19*25 angaphandle kwebanga lokutholwa kwale kit akunawo umthelela emthelela wokutholwa wale khithi.
Izinsimbi Ezisebenzayo I-Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

I-QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

I-LineGene 9600 Plus Real-Time PCR Detection System

I-MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Real-Time PCR System

I-BioRad CFX Opus 96 Real-Time PCR System

Ukugeleza komsebenzi

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Isizinda kufanele sikhishwe ngokulandela imiyalelo.Ivolumu yesampula yokukhipha ingu-200μL, futhi ivolumu ye-lution enconyiwe ingu-100μL.

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Wizard® Genomic DNA Purification Kit (Ikhathalogi No.: A1120) nge-Promega, i-Nucleic Acid Extraction noma i-Purification Reagent(YDP348) yi-Tiangen Biotech(Beijing) Co.,Ltd.kufanele ikhishwe ngokulandela imiyalelo yokukhipha, futhi ivolumu yokukhipha enconyiwe ingu-200 μL futhi ivolumu ye-elution enconyiwe ingu-160 μL.


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