Izinhlobo ezingu-28 ze-Human Papillomavirus (16/18/45Typing) Ikhithi Yokuthola I-Nucleic Acid (i-Fluorescence PCR)

Incazelo emfushane:

[UmkhiqizoIkhodi] I-HWTS-CC024

[Ukusetshenziswa Okuhlosiwe]

Le khithi ifanelekela ukutholwa kwe-nucleic acid kwezinhlobo ezingu-28 ze-human papillomavirus (HPV 6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83)skuukwelashwaumchamoisampula,isampula ye-swab yomlomo wesibeletho yowesifazane kanye nesampula ye-swab yowesifazane wesibelethokanye ne-HPV 16/18/45 ukutholwa kokuthayipha, ngenkathiizinhlobo ezisele azikwazi ukuba ngokupheleleovelele, ukuhlinzekausizo kuukuxilongwa nokwelashwa kokutheleleka kwe-HPV.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Izinhlobo ezingu-28 ze-Human Papillomavirus (16/18/45Typing) Ikhithi Yokuthola I-Nucleic Acid (i-Fluorescence PCR)

I-Epidemiology

I-Human Papillomavirus (HPV) ingeyomndeni we-Papillomaviridae wegciwane le-DNA eliyindilinga elinemigqa emibili, elingamboziwe, elinobude be-genome obungaba yizibili eziyisisekelo (bp) ezingu-8000. I-HPV ithelela abantu ngokuxhumana okuqondile noma okungaqondile nezinto ezingcolisiwe noma ukudluliselwa ngokocansi. Leli gciwane aligcini nje ngokuba ngelakhe, kodwa futhi liqondene nezicubu, futhi lingathelela kuphela isikhumba somuntu kanye namaseli e-epithelial e-mucosal, okubangela izinhlobo ezahlukene zama-papillomas noma ama-warts esikhumbeni somuntu kanye nomonakalo owandayo ku-epithelium yomgudu wokuzala.

Umdlavuza womlomo wesibeletho ungenye yezimila eziyingozi ezivame kakhulu ohlelweni lokuzala lwabesifazane. Izifundo zikhombisile ukuthi ukutheleleka okuqhubekayo kwe-HPV kanye nokutheleleka okuningi kungenye yezimbangela eziyinhloko zomdlavuza womlomo wesibeletho. Njengamanje, ukwelashwa okusebenzayo okuqashelwayo kusantuleka komdlavuza womlomo wesibeletho obangelwa yi-HPV, ngakho-ke ukutholakala kusenesikhathi nokuvimbela ukutheleleka komlomo wesibeletho okubangelwa yi-HPV kuyisihluthulelo sokuvimbela umdlavuza womlomo wesibeletho. Kubaluleke kakhulu ukusungula isivivinyo sokuxilonga esilula, esiqondile nesisheshayo se-etiology sokuxilongwa kanye nokwelashwa komdlavuza womlomo wesibeletho.

Amapharamitha Obuchwepheshe

Isitoreji	≤ -18℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	Ui-rineisampula,Fisampula ye-emale cervical swab, Fisampula ye-emale vaginal swab
CV	<5.0%
I-LoD	Umkhawulo Wokuthola (LoD): I-LoD yekhithi ingu-300Cama-opioid/mL
Ukucaciswa	Akukho ukusabela okuphambenengezifo ezifanayo.
Izinsimbi Ezisebenzayo	Uhlelo lwe-Applied Biosystems 7500 Real-Time PCR, Izinhlelo ze-Applied Biosystems 7500 Fast Real-Time PCR, Izinhlelo ze-QuantStudio®5 Real-Time PCR, Izinhlelo ze-SLAN-96P Real-Time PCR (Hongshi Medical Technology Co., Ltd.), Izinhlelo ze-LightCycler®480 Real-Time PCR, Izinhlelo ze-LineGene 9600 Plus Real-Time PCR Detection (FQD-96A,I-HangzhouUbuchwepheshe be-Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), i-BioRad CFX96 Real-Time PCR System kanye ne-BioRad CFX Opus 96 Real-Time PCR System.

Isiteshi:

I-Reaction Buffer	I-fluorophore	Isicimi
I-PCR-Mix1/PCR-Mix2	I-FAM	akukho
	I-VIC(I-HEX)	akukho
	I-ROX	akukho
	CY5	akukho

Ukugeleza komsebenzi: Bheka i-Kit IFU ukuthola imininingwane

Isixazululo 1 (Ukukhishwa kwe-nucleic acid okuzenzakalelayo)

Isixazululo 2: (I-Macro & Micro-Test Sample Release Release Reagent)

Ama-reagent adinga kodwa awanikezwa:I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ikholomu ye-Macro & Micro-Test Viral DNA/RNA (HWTS-3020-50).

Izinto ezisetshenziswayo zidinga kodwa azinikezwa: 1.5mL DNase/RNase ayinayoci-entrifugetama-ube,DImpumulo/RAma-Tips angenawo ama-Nase, amashubhu e-PCR angu-0.2mL, i-desktop centrifuge, i-desktop oscillation mixer.