Ikhithi Yokuthola I-Chlamydia Trachomatis Nucleic Acid (i-Fluorescence PCR)

Isithombe Esifakiwe Sekhithi Yokuthola I-Chlamydia Trachomatis Nucleic Acid (Fluorescence PCR)

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola i-Chlamydia trachomatis nucleic acid esezingeni eliphezulu kumchamo wesilisa, i-urethral swab yesilisa, kanye namasampula e-cervical swab yesifazane.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama Lomkhiqizo

Ikhithi Yokuthola I-Chlamydia Trachomatis Nucleic Acid (i-Fluorescence PCR)

Ukusetshenziswa Okuhlosiwe

Le khithi isetshenziselwa ukuthola i-Chlamydia trachomatis nucleic acid esezingeni eliphezulu kumchamo wesilisa, i-urethral swab yesilisa, kanye namasampula e-cervical swab yesifazane.

I-Epidemiology

I-Chlamydia trachomatis (CT) uhlobo lwe-prokaryotic microorganism evame ukuba yi-parasitic kumaseli e-eukaryotic. I-Chlamydia trachomatis ihlukaniswe ngama-serotype e-AK ngokwendlela ye-serotype. Ukutheleleka kwe-Urogenital tract kubangelwa kakhulu yi-trachoma biological variant DK serotypes, kanti abesilisa bavame ukubonakala njenge-urethritis, engaqedwa ngaphandle kokwelashwa, kodwa iningi labo liba yi-chronic, liba libi ngezikhathi ezithile, futhi lingahlanganiswa ne-epididymitis, i-proctitis, njll. Abesifazane bangabangelwa yi-urethritis, i-cervicitis, njll., kanye nezinkinga ezinkulu ze-salpingitis.

I-Epidemiology

FAM: Chlamydia trachomatis (CT)·

I-VIC(HEX): Ukulawula Kwangaphakathi

Ukusetha Izimo Zokukhulisa ze-PCR

Isinyathelo	Imijikelezo	Izinga lokushisa	Isikhathi	Qoqa Izimpawu Zokukhanya Noma Cha
1	Umjikelezo ongu-1	50℃	imizuzu emi-5	No
2	Umjikelezo ongu-1	95℃	imizuzu eyi-10	No
3	imijikelezo engu-40	95℃	imizuzwana eyi-15	No
4	imijikelezo engu-40	58℃	amasekhondi angu-31	Yebo

Amapharamitha Obuchwepheshe

Isitoreji	≤-18℃ Ebumnyameni
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	Ukukhishelwa komchamo kwabesilisa, Ukukhishelwa komlomo wesibeletho kwabesifazane, Umchamo wabesilisa
Ct	≤38
CV	＜5.0%
I-LoD	Amakhophi angu-400/mL
Ukucaciswa	Akukho ukusabela okuxubile ekutholeni ezinye izifo ezitheleleke nge-STD ngale kit, njenge-Treponema pallidum, i-Neisseria gonorrhoeae, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium, njll., ezingaphandle kobubanzi bokutholwa kwekit.
Izinsimbi Ezisebenzayo	Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio^®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler^®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR

Ukuhamba komsebenzi

Ikhithi Yokuthola I-Chlamydia Trachomatis Nucleic Acid (i-Fluorescence PCR)

Ama-reagent adinga kodwa awanikezwa

Ikhithi Yokuqoqa Isampula Ejwayelekile, I-Macro & Micro-Test, Iposi Nokuthumela (HWTS-3030-1B), Ikhithi Ye-DNA/RNA Yegciwane Le-Macro & Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), i-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co., Ltd.

Izinto ezisetshenziswayo ziyadingeka kodwa azinikezwa

Amathiphu angenazo i-DNase/RNase, Amagilavu alahlwayo, amashubhu e-EP angenazo i-DNase/RNase, i-Centrifuge, i-Magnetic stand, imichilo enamashubhu angu-8 ye-qPCR esekelwe ku-fluorescence.

Okwedlule: Ikhithi Yokuthola I-Herpes Simplex Virus Type 2 Nucleic Acid (i-Fluorescence PCR)

Olandelayo: Ikhithi Yokuthola I-Trichomonas Vaginalis Nucleic Acid (i-Fluorescence PCR)

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