Izinhlobo ezingu-29 zezifo zokuphefumula i-Combined Nucleic Acid
Igama lomkhiqizo
I-HWTS-RT160 -29 Izinhlobo Zamagciwane Okuphefumula Ikhithi Yokuthola I-Nucleic Acid Ehlanganisiwe
I-Epidemiology
Ukutheleleka ngomgudu wokuphefumula yisifo esivame kakhulu kubantu, esingaba khona kunoma yiluphi ubulili, ubudala kanye nesifunda. Kungenye yezimbangela eziphambili zokugula nokufa kwabantu emhlabeni wonke[1]. Izifo ezivamile zokuphefumula zifaka phakathi i-coronavirus entsha, igciwane le-Influenza A, igciwane le-Influenza B, igciwane le-Respiratory syncytial, i-Adenovirus, i-human metapneumovirus, i-rhinovirus, igciwane le-Parainfluenza uhlobo I/II/III, i-Bocavirus, i-Enterovirus, i-Coronavirus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, kanye ne-Streptococcus pneumoniae, njll.[2,3]. Izimpawu nezimpawu ezibangelwa ukutheleleka kokuphefumula ziyafana, kodwa izindlela zokwelapha, ukusebenza kahle kanye nendlela yokutheleleka okubangelwa ukutheleleka okubangelwa ukutheleleka okuhlukahlukene kuhlukile[4,5]. Njengamanje, izindlela eziyinhloko ezisetshenziswa emalabhorethri ukuthola ukutheleleka kokuphefumula okukhulunywe ngakho ngenhla zifaka: ukuhlukaniswa kwegciwane, ukutholwa kwe-antigen kanye nokutholwa kwe-nucleic acid, njll. Le khithi ithola futhi ihlonze ama-virtual nucleic acid athile kubantu abanezimpawu nezimpawu zokutheleleka kokuphefumula, ngokutholwa kokuthayipha kwamagciwane omkhuhlane kanye nama-coronavirus, futhi ihlangana neminye imiphumela yelabhorethri ukunikeza usizo ekuxilongweni kokutheleleka kwezifo zokuphefumula. Imiphumela emibi ayikukhiphi ukutheleleka ngegciwane lokuphefumula futhi akufanele isetshenziswe njengesisekelo sodwa sokuxilongwa, ukwelashwa, noma ezinye izinqumo zokuphatha. Umphumela omuhle awukwazi ukuqeda ukutheleleka ngamagciwane noma ukutheleleka okuxubile ngamanye amagciwane okungaphandle kwezinkomba zokuhlola. Abaqhubi bokuhlola kufanele ngabe bathole ukuqeqeshwa kobungcweti ekukhuliseni izakhi zofuzo noma ekutholeni i-molecular biology, futhi babe neziqu zokusebenza kokuhlola ezifanele. Ilabhorethri kufanele ibe nezindawo zokuvimbela ukuphepha kwe-biosafety ezifanele kanye nezinqubo zokuvikela.
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Amakhophi angu-200/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kwe-cross-reactivity ikhombisile ukuthi bekungekho ukusabela okuhlangene phakathi kwale kit ne-Cytomegalovirus, uhlobo lwegciwane le-Herpes simplex 1, igciwane le-Varicella-zoster, igciwane le-Epstein-Barr, i-Pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella pneumophila, i-Moraxella catarrhalis, izinhlobo ezincishisiwe zesifo sofuba se-Mycobacterium, i-Neisseria meningitidis, i-Neisseria, i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Staphylococcus epidermidis, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Acinetobacter baumannii, i-Stenotrophomonas maltophilia, i-Burkholderia cepacia, i-Corynebacterium striatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis jiroveci, i-Candida albicans, I-Rothia mucilaginosus, i-Streptococcus oralis, i-Klebsiella pneumoniae, i-Chlamydia psittaci, i-Coxiella burnetii, kanye nama-nucleic acid e-genomic yabantu. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time,Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480, Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-EudemonTM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.
