Izinhlobo ezingama-29 zama-Pathogens Okuphefumula Ahlanganiswe I-Nucleic Acid
Igama lomkhiqizo
I-HWTS-RT160 -29 Izinhlobo Zamagciwane Aphefumulayo Ahlanganiswe Ikhithi Yokutholwa Kwe-Nucleic Acid
I-Epidemiology
Ukutheleleka komgudu wokuphefumula yisifo esivame kakhulu kubantu, esingenzeka kunoma yibuphi ubulili, ubudala kanye nesifunda. Ingenye yezimbangela eziphambili zokugula nokufa kwabantu emhlabeni wonke[1]. Amagciwane avamile okuphefumula ahlanganisa inoveli i-coronavirus, igciwane loMkhuhlane A, igciwane lomkhuhlane B, i-Respiratory syncytial virus, i-Adenovirus, i-human metapneumovirus, i-rhinovirus, i-Parainfluenza virus hlobo I/II/III, i-Bocavirus, i-Enterovirus, iCoronavirus, i-Mycoplasma pneumoniae, i-Chlamydia pneumonia, i-Streptococcus] Izimpawu nezimpawu ezibangelwa izifo zokuphefumula ziyafana, kodwa izindlela zokwelapha, ukusebenza kahle kanye nenkambo yezifo ezibangelwa amagciwane ahlukene zihlukile[4,5]. Njengamanje, izindlela eziyinhloko ezisetshenziswa ezindaweni zokucwaninga ukuze kutholwe amagciwane ashiwo ngenhla okuphefumula ahlanganisa: ukuhlukaniswa kwegciwane, ukutholwa kwe-antigen kanye nokutholwa kwe-nucleic acid, njll. Le kit ithola futhi ihlonze ama-nucleic acid athile egciwane kubantu abanezimpawu nezimpawu zokutheleleka kokuphefumula, ngokuthayipha ukutholwa kwamagciwane omkhuhlane kanye nama-coronavirus, futhi ihlangana neminye imiphumela ye-pathogen yokuphefumula. Imiphumela engemihle ayibandakanyi ukutheleleka ngegciwane lokuphefumula futhi akufanele isetshenziswe njengesisekelo sodwa sokuxilongwa, ukwelashwa, noma ezinye izinqumo zokuphatha. Umphumela omuhle awukwazi ukukhipha izifo ezibangelwa amagciwane noma izifo ezixutshwe ngamanye amagciwane angaphandle kwezinkomba zokuhlola. Abasebenzi bokuhlola kufanele ukuthi bathole ukuqeqeshwa kochwepheshe ekukhuliseni izakhi zofuzo noma ukutholwa kwebhayoloji yamangqamuzana, futhi babe neziqu ezifanele zokusebenza kokuhlola. Ilabhorethri kufanele ibe nezindawo zokuvimbela ukuphepha kwemvelo ezinengqondo kanye nezinqubo zokuvikela.
Imingcele Yezobuchwepheshe
| Isitoreji | -18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Amakhophi/μL |
| Ukucaciswa | Imiphumela yokuhlolwa kwe-cross-reactivity ibonise ukuthi kwakungekho ukusabela okuphambene phakathi kwaleli kit kanye ne-Cytomegalovirus, i-Herpes simplex virus uhlobo 1, i-Varicella-zoster virus, i-Epstein-Barr virus, i-Pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella pneumotarulixels, i-Mylatten bacteria isifo sofuba, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophophimonacemonas malkhoumonia bacteria i-striatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis jiroveci, i-Candida albicans, i-Rothia mucilaginosus, i-Streptococcus oralis, i-Klebsiella pneumoniae, i-Klebsiella pneumoniae, i-Chlacitimic pneumoniae, i-Colamydia pneumola ama-asidi. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, I-QuantStudio®5 Amasistimu e-PCR wesikhathi sangempela, I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), I-LightCycler®480 uhlelo lwe-PCR lwesikhathi sangempela, I-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), I-BioRad CFX96 Real-Time PCR System, I-BioRad CFX Opus 96 Real-Time PCR System. |
Ukugeleza komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-17u-80 esetshenziswa)TM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ivolumu yesampula ekhishiwe ingu-200μL futhi ivolumu ye-lution enconyiwe ingu-150μL.
