Izinhlobo ezi-4 zamagciwane okuphefumula aucleic acid

Izinhlobo ezi-4 zama-virus okuphefumula ze-nucleic acid ezifakiwe

Incazelo emfushane:

Le khithi isetshenziselwa ukutholwa kwe-SARS-cov-2, umkhuhlane wegciwane, umkhuhlane B virus kanye namagciwane ancordlial aucytial amasampula.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-RT075-Qhanda Izinhlobo ezi-4 zama-virus zokuphefumula ikhithi yokuphefumula ye-nucleic acid detection (Fluorescence PCR)

I-Epidemiology

Isifo segciwane leCorona rus ka-2019, okukhulunywa ngaye ngokuthi "Covid-19", kubhekiswa ku-pneumonia okubangelwa ukutheleleka kwe-SARS-Cov-2. AbakwaSars-Cov-2 yi-coronavirus eyi-β runes. I-COVIIG-19 yisifo esithelelekayo sokuphefumula esinamandla, futhi inani labantu livame ukwenzeka. Njengamanje, umthombo wokutheleleka uneziguli ikakhulukazi ezitheleleke yi-SARS-Cov-2, futhi abantu abanegciwane le-asymptomatic atheleleke nabo bangaba ngumthombo wokutheleleka. Ngokusekelwe ophenyweni lwezempi lwamanje, isikhathi sokufakwa kwezinsuku eziyi-1-14, iningi lezinsuku ezingama-3-7. Umkhuhlane, ukukhwehlela owomile nokukhathala kungukubonakaliswa okuyinhloko. Iziguli ezimbalwa zazinezimpawu ezifana nokuxinana kwamakhala, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nohudo, njll.

Umkhuhlane, ojwayele owaziwa ngokuthi "umkhuhlane", yisifo esibuhlungu sokuphefumula esidalwa yigciwane lomkhuhlane. Kuyatheleleka kakhulu. Idluliselwa ikakhulukazi ngokukhwehlela nokushushuluza. Imvamisa ihlukana entwasahlobo nasebusika. Amagciwane omkhuhlane ahlukaniswe yaba ngumkhuhlane a -I-RNA Virus ehlukanisiwe. Umkhuhlane Igciwane lingukutheleleka okuphezulu kokuphefumula, kufaka phakathi i-H1N1, H3N2 namanye ama-subtypes, athambekele ekuguqukeni nasekuqubukeni emhlabeni wonke. I- "Shift" ibhekisela ekuguqukeni komkhuhlane ngegciwane, okuholele ekuqubukeni kwegciwane elisha elithi "subtype". Umkhuhlane B Amagciwane ahlukaniswe ngamabala amabili, iYamagata neVictoria. Umkhuhlane B Virus kuphela une-antiigenic drift, futhi kuthola ukubhekwa kwamasosha omzimba womuntu nokuqedwa ngokuguqulwa kwayo. Kodwa-ke, ijubane lokuziphendukela kwemvelo le-LogeENENeza B Virus lihamba kancane kunaleso somkhuhlane womuntu igciwane. Igciwane le-Informen B lingabuye libangele izifo zokuphefumula komuntu futhi ziholele ezinhlanzini.

Igciwane le-respiratory syncytial virus (RSV) yigciwane le-RNA, elingelamndeni wePharamyxoviridae. Kudluliselwa ngamaconsi amoya kanye nokuxhumana okusondelene futhi yi-pathogen eyinhloko yokutheleleka okuphansi kokuphefumula kwezingane. Izinsana ezitheleleke nge-RSV zingahlakulela i-bronchiolitis enzima ne-pneumonia, ezihlobene nesifuba sezinto zomoya. Izinsana zinezimpawu ezinzima, kufaka phakathi umkhuhlane ophakeme, i-rhinitis, i-pharyngitis kanye ne-laryngitis, bese kuthi i-bronchiolitis ne-pneumonitia. Izingane ezimbalwa ezigula zingaba yinkimbinkimbi ngemidiya ye-Otitis, i-Pleurisy naseMyOCarditis, njll. Upper Upper Respiratory Tract Tract uwuphawu oluphambili lokutheleleka kubantu abadala nasezinganeni ezindala.

Insungubezi

-Yenza	I-SARS-CoV-2
I-VIC (Hex)	I-RSV
Cy5	IFV a
Ivupho	IFV B
Ind	Ukulawulwa Kwangaphakathi

Amapharamitha Ezobuchwepheshe

Ukubeka	2-8 ° C
Ishalofu-impilo	Izinyanga eziyi-12
Uhlobo lwe-Specimen	I-Oropharyngengeal Swapy
Ct	≤38
Indlu edayisa insimbi	I-SARS-COV-2: 150Copies / ML Umkhuhlane I-Virus / Umkhuhlane B Virus / Ukuphefumula kwegciwane lokuvumelanisa: 300Copies / ML
Ukucacisa	Akukho okuphambana nokusebenzisa imali nge-coronavirus sarsr-cov, i-Merrs-Cov, HCOV-OC43, HCOV-NL63, HCOV-NL63, B, 3, CHAMIMDIA inyumoniae, i-metapneovirus yomuntu, i-elovierus a, b, c, D, igciwane le-Human pulmonary, igciwane le-exstein-barl, igciwane lesimungumungwane, i-sount cytomegalo virus, i-athovirus, igciwane le-parotitis, i-abuemophilus aureus, i-steptococcus pneumoniae ,, i-streptococcus pneumoniae, s. Ama-Pyogene, Klebsaella Pneumoniae, i-Mycobacterium Tuberculosis, i-Smoke Aspergillus, ama-Albicans we-Candica, i-Candida Glabrata, i-pneumocystis jiroveci ne-cryptococcus entsha ne-genomic nucleic acid.
Izinsimbi ezisebenzayo	I-Biosystems i-Biosystems 7500 uhlelo lwe-real-time PCR, lusebenzisa ama-biosystems 7500 ngokushesha amasistimu we-PCR yesikhathi sangempela, i-QuantStudio®5