Izinhlobo ezi-4 Zamagciwane Okuphefumula I-Nucleic Acid

Incazelo emfushane:

Le khithi isetshenziselwa ukutholwa kwekhwalithi ye-SARS-CoV-2, igciwane lomkhuhlane A, igciwane lomkhuhlane B kanye ne-respiratory syncytial virus nucleic acids kumasampula e-oropharyngeal swab.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-RT186- 4 Izinhlobo Zamagciwane Okuphefumula I-Nucleic Acid Detection Kit (Fluorescence PCR)

I-Epidemiology

I-Corona Virus Disease 2019, ebizwa ngokuthi “i-COVID-19”, isho inyumoniya ebangelwa ukutheleleka kwe-SARS-CoV-2. I-SARS-CoV-2 iyi-coronavirus yohlobo lwe-β. I-COVID-19 yisifo esithathelwanayo esinamandla sokuphefumula, futhi inani labantu ngokuvamile liyathinteka. Njengamanje, umthombo wokutheleleka ikakhulukazi iziguli ezingenwe yi-SARS-CoV-2, futhi abantu abangenwe yi-asymptomatic bangase babe umthombo wokutheleleka. Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezingu-1-14, ikakhulukazi izinsuku ezingu-3-7. Umkhuhlane, ukukhwehlela okomile nokukhathala yizimpawu eziyinhloko. Iziguli ezimbalwa zazinezimpawu ezinjengokuminyana emakhaleni, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo, njll.

Umkhuhlane, owaziwa ngokuthi "umkhuhlane", isifo esithathelwanayo sokuphefumula esibangelwa igciwane lomkhuhlane. Iyathelelana kakhulu. Isakazwa kakhulu ngokukhwehlela kanye nokuthimula. Ivamise ukuqubuka entwasahlobo nasebusika. Amagciwane omkhuhlane we-influenza ahlukaniswe abe umkhuhlane A (IFV A), umkhuhlane B (IFV B), kanye no-C (IFV C) izinhlobo ezintathu, zonke zingezamagciwane anamathelayo, abangela isifo somuntu ikakhulukazi kumagciwane omkhuhlane A kanye no-B, iyigciwane le-RNA elinomugqa owodwa, elihlukaniswe izingxenye. Igciwane loMkhuhlane A liwukutheleleka kokuphefumula okunamandla, okuhlanganisa i-H1N1, i-H3N2 nezinye izinhlobo ezincane, ezijwayele ukuguquka nokuqubuka emhlabeni jikelele. I-"Shift" ibhekisela ekuguqulweni kwegciwane lomkhuhlane A, okuholela ekuveleni kwegciwane elisha "i-subtype". Amagciwane oMkhuhlane B ahlukaniswe izigaba ezimbili, i-Yamagata ne-Victoria. Igciwane lomkhuhlane B line-antigenic drift kuphela, futhi ligwema ukubhekwa nokuqedwa kwamasosha omzimba ngokuguqulwa kwawo. Kodwa-ke, ijubane lokuvela kwegciwane lomkhuhlane B lihamba kancane kunelo legciwane le-human influenza A. Igciwane loMkhuhlane B lingase futhi libangele izifo zokuphefumula komuntu futhi libangele izifo eziwumshayabhuqe.

I-Respiratory syncytial virus (RSV) igciwane le-RNA, eliyingxenye yomndeni we-paramyxoviridae. Idluliselwa ngamaconsi omoya kanye nokuthintana eduze futhi iyi-pathogen eyinhloko yezifo eziphansi zokuphefumula ezinsaneni. Izinsana ezingenwe i-RSV zingase zibe ne-bronchiolitis enamandla nenyumoniya, okuhlobene nesifuba somoya ezinganeni. Izinsana zinezimpawu ezinzima, ezihlanganisa ukushisa okukhulu, i-rhinitis, i-pharyngitis ne-laryngitis, bese kuba yi-bronchiolitis ne-pneumonia. Izingane ezimbalwa ezigulayo zingaba yinkimbinkimbi nge-otitis media, i-pleurisy ne-myocarditis, njll.

Isiteshi

FAM	I-SARS-CoV-2
I-VIC(HEX)	I-RSV
CY5	IFV A
I-ROX	IFV B
I-NED	Ukulawula Kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji	2-8°C
Impilo yeshelufu	Izinyanga ezingu-12
Uhlobo lwesifanekiso	I-Oropharyngeal swab
Ct	≤38
LoD	I-SARS-CoV-2: 150Copies/mL Igciwane loMkhuhlane/Igciwane loMkhuhlane B/Igciwane le-Respiratory syncytial: 300Copies/mL
Ukucaciswa	Akukho ukuphinda kusebenze nge-coronavirus yabantu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, i-parainfluenza virus hlobo 1, 2, 3, i-rhinovirus A, B, C, i-chlamydia pneumoniaeumoeumo, i-virus yomuntu, i-metapneumoviruse, i-human igciwane lamaphaphu, igciwane le-epstein-barr, igciwane lesimungumungwane, igciwane le-cytomegalo, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, i-legionella, i-bordetella pertussis, i-haemophilus influenzae, i-staphylococcus aureus, i-streptococcus pneumoniae, i-s. pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kanye ne-cryptococcus esanda kuzalwa kanye ne-nucleic acid yomuntu.
Izinsimbi Ezisebenzayo	I-Applied Biosystems 7500 Real-Time PCR System, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems

Ukugeleza komsebenzi

Inketho 1.

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Inketho yesi-2.

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: Isizinda se-Nucleic Acid noma i-Purification Reagent(YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.

Okwedlule: I-Mycobacterium Tuberculosis Rifampicin Resistance

Olandelayo: Izinhlobo eziyi-19 zePathogen Nucleic Acid Yokuphefumula

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