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    • Izinhlobo Ezi-4 Zamagciwane Okuphefumula I-Nucleic Acid

    Izinhlobo Ezi-4 Zamagciwane Okuphefumula Isithombe Esifakiwe Se-Nucleic Acid
    • Izinhlobo Ezi-4 Zamagciwane Okuphefumula I-Nucleic Acid

    Incazelo emfushane:

    Le khithi isetshenziselwa ukuthola i-SARS-CoV-2, igciwane le-influenza A, igciwane le-influenza B kanye ne-nucleic acids yegciwane lokuphefumula elihambisanayo kumasampula e-human oropharyngeal swab.


    Imininingwane Yomkhiqizo

    Amathegi Omkhiqizo

    Igama lomkhiqizo

    I-HWTS-RT186- Izinhlobo Ezi-4 Zamagciwane Okuphefumula I-Nucleic Acid Detection Kit (i-Fluorescence PCR)

    I-Epidemiology

    Isifo Segciwane Le-Corona sika-2019, esibizwa ngokuthi "i-COVID-19", sibhekisela e-pneumonia ebangelwa ukutheleleka kwe-SARS-CoV-2. I-SARS-CoV-2 iyi-coronavirus eyingxenye yohlobo lwe-β. I-COVID-19 iyisifo esithathelwanayo sokuphefumula esibukhali, futhi abantu ngokuvamile bayathinteka. Njengamanje, umthombo wokutheleleka ikakhulukazi yiziguli ezitheleleke yi-SARS-CoV-2, kanti abantu abanegciwane abangenazo izimpawu nabo bangaba umthombo wokutheleleka. Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku eziyi-1-14, ikakhulukazi izinsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomile kanye nokukhathala yizimpawu eziyinhloko. Iziguli ezimbalwa zazinezimpawu ezifana nokuminyana kwamakhala, ikhala eligobhozayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo, njll.

    I-Influenza, eyaziwa kakhulu ngokuthi "i-flu", iyisifo esithathelwanayo sokuphefumula esibangelwa yigciwane le-influenza. Ithelelana kakhulu. Idluliselwa kakhulu ngokukhwehlela nokuthimula. Ivame ukuqubuka entwasahlobo nasebusika. Amagciwane e-Influenza ahlukaniswe izinhlobo ezintathu ze-influenza A (IFV A), i-influenza B (IFV B), kanye ne-Influenza C (IFV C), zonke zingegciwane elinamathelayo, elibangela izifo kubantu ikakhulukazi amagciwane e-influenza A kanye ne-B, iyigciwane le-RNA elinomugqa owodwa, elihlukaniswe ngezigaba. Igciwane le-Influenza A liyigciwane lokuphefumula elinamandla, okuhlanganisa i-H1N1, i-H3N2 kanye nezinye izinhlobo, ezivame ukuguquka nokuqhuma emhlabeni jikelele. Igama elithi "Shift" libhekisela ekuguqukeni kwegciwane le-influenza A, okuholela ekuveleni kwegciwane elisha "elingaphansi". Amagciwane e-Influenza B ahlukaniswe ngezigaba ezimbili, i-Yamagata ne-Victoria. Igciwane le-Influenza B linokuhamba kwe-antigenic kuphela, futhi ligwema ukubhekwa nokususwa kwesistimu yomzimba womuntu ngokuguqulwa kwalo. Kodwa-ke, ijubane lokuvela kwegciwane le-influenza B lihamba kancane kunelegciwane le-influenza A lomuntu. Igciwane le-Influenza B lingabangela nezifo zokuphefumula kubantu futhi liholele ezifweni eziwumshayabhuqe.

    Igciwane le-Respiratory syncytial (RSV) liyigciwane le-RNA, elivela emndenini we-paramyxoviridae. Lidluliselwa ngamaconsi omoya kanye nokuxhumana okuseduze futhi liyimbangela eyinhloko yokutheleleka kwepheshana lokuphefumula eliphansi ezinganeni. Izinsana ezinegciwane le-RSV zingase zibe ne-bronchiolitis enzima kanye ne-pneumonia, okuhlobene ne-asthma ezinganeni. Izinsana zinezimpawu ezinzima, okuhlanganisa umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kanye ne-laryngitis, bese kuba yi-bronchiolitis kanye ne-pneumonia. Izingane ezimbalwa ezigulayo zingaba yinkimbinkimbi nge-otitis media, i-pleurisy kanye ne-myocarditis, njll. Ukutheleleka kwepheshana lokuphefumula eliphezulu kuyisibonakaliso esiyinhloko sokutheleleka kubantu abadala kanye nezingane ezindala.

    Isiteshi

    I-FAM I-SARS-CoV-2
    I-VIC(HEX) I-RSV
    CY5 I-IFV A
    I-ROX I-IFV B
    I-NED

    Ukulawula Kwangaphakathi

    Amapharamitha Obuchwepheshe

    Isitoreji 2-8°C
    Isikhathi sokuphelelwa yisikhathi Izinyanga ezingu-12
    Uhlobo Lwesifanekiso I-Oropharyngeal swab
    Ct ≤38
    I-LoD I-SARS-CoV-2: Amakhophi angu-150/mL

    Igciwane le-Influenza A/Igciwane le-Influenza B/Igciwane le-Respiratory syncytial: 300 Amakhophi/mL
    Ukucaciswa Akukho ukuhlangana kwegciwane le-coronavirus yomuntu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, igciwane le-parainfluenza uhlobo 1, 2, 3, i-rhinovirus A, B, C, i-chlamydia pneumoniae, i-human metapneumovirus, i-enterovirus A, B, C, D, igciwane le-pulmonary lomuntu, igciwane le-epstein-barr, igciwane le-measles, igciwane le-cytomegalo lomuntu, i-rotavirus, i-norovirus, igciwane le-parotitis, igciwane le-varicella-zoster, i-legionella, i-bordetella pertussis, i-haemophilus influenzae, i-staphylococcus aureus, i-streptococcus pneumoniae, i-s. pyogenes, i-klebsiella pneumoniae, i-mycobacterium tuberculosis, i-smoke aspergillus, i-candida albicans, i-candida glabrata, i-pneumocystis jiroveci kanye ne-cryptococcus esanda kuzalwa kanye ne-human genomic nucleic acid.
    Izinsimbi Ezisebenzayo Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time, Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time, Izinhlelo ze-PCR ze-QuantStudio®5 Real-Time

    Ukugeleza Komsebenzi

    Inketho 1.

    I-reagent yokukhipha enconywayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

    Inketho 2.

    I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co.,Ltd.

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