I-Chlamydia Pneumoniae Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-RT023-Chlamydia Pneumoniae Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Ukutheleleka kwe-Acute Respiratory Tract Infection (ARTI) yisifo esivamile esiphindaphindayo ezinganeni, phakathi kwaso i-Chlamydia pneumoniae kanye nokutheleleka kwe-Mycoplasma pneumoniae kuyibhaktheriya evamile ye-pathogenic futhi inokutheleleka okuthile, futhi ingadluliselwa ngomgudu wokuphefumula ngamaconsi. Izimpawu zincane, ikakhulukazi ngomphimbo obuhlungu, ukukhwehlela okomile, kanye nomkhuhlane, kanti izingane zabo bonke ubudala ziyathinteka. Inani elikhulu lemininingwane libonisa ukuthi izingane zesikole ezingaphezu kweminyaka engu-8 kanye nabantu abasha yibo abathinteka yi-Chlamydia pneumoniae, okubalwa cishe yi-10-20% ye-pneumonia etholakala emphakathini. Iziguli ezindala ezine-immune system ephansi noma izifo eziyisisekelo nazo ziyathinteka yilesi sifo. Eminyakeni yamuva nje, izinga lokugula lokutheleleka kwe-Chlamydia pneumoniae liye landa unyaka nonyaka, kanti izinga lokutheleleka phakathi kwezingane zasenkulisa nezasesikoleni liphezulu. Ngenxa yezimpawu zokuqala ezingavamile kanye nesikhathi eside sokukhulelwa kokutheleleka kwe-Chlamydia pneumoniae, amazinga okuxilonga okungalungile kanye namazinga okuxilonga angatholakali aphezulu ekuxilongweni kwezokwelapha, ngaleyo ndlela kubambezele ukwelashwa kwezingane.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | isikhwehlela, i-oropharyngeal swab |
| CV | ≤10.0% |
| I-LoD | Amakhophi angu-200/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kwe-cross-reactivity ibonise ukuthi akukho ukusabela okuhlangene phakathi kwale kit ne-Ureaplasma urealyticum, i-Mycoplasma genitalium, i-Mycoplasma hominis, i-Streptococcus pneumoniae, i-Mycoplasma pneumoniae, i-Haemophilus influenzae, i-Klebsiella pneumoniae, i-Staphylococcus aureus, i-Mycobacterium tuberculosis, i-Legionella pneumophila, i-Pseudomonas aeruginosa, i-Acinetobacter baumannii, igciwane le-influenza A, igciwane le-influenza B, igciwane le-Parainfluenza uhlobo lwe-I/II/III/IV, i-Rhinovirus, i-Adenovirus, i-human metapneumovirus, igciwane le-respiratory syncytial kanye ne-human genomic nucleic acids. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480, Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-EudemonTM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.
