I-Chlamydia Trachomatis, i-Neisseria Gonorrhoeae kanye ne-Trichomonas vaginalis
Igama lomkhiqizo
I-HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae kanye ne-Trichomonas vaginalis Nucleic Acid Detection Kit (i-Fluorescence PCR)
I-Epidemiology
I-Chlamydia trachomatis (CT) uhlobo lwe-prokaryotic microorganism evame ukuba yi-parasitic kumaseli e-eukaryotic. I-Chlamydia trachomatis ihlukaniswe ngama-serotype e-AK ngokwendlela ye-serotype. Ukutheleleka kwe-Urogenital tract kubangelwa kakhulu yi-trachoma biological variant DK serotypes, kanti abesilisa bavame ukubonakala njenge-urethritis, engaqedwa ngaphandle kokwelashwa, kodwa iningi labo liba yi-chronic, liba libi ngezikhathi ezithile, futhi lingahlanganiswa ne-epididymitis, proctitis, njll.
Isiteshi
| I-FAM | I-Chlamydia trachomatis |
| I-ROX | I-Neisseria gonorrhoeae |
| CY5 | I-Trichomonal vaginitis |
| I-VIC/HEX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomlomo wesibeletho yowesifazane,I-swab yesitho sangasese sowesifazane,I-swab ye-urethra yabesilisa |
| Ct | ≤38 |
| CV | <5% |
| I-LoD | 400Amakhophi/mL |
| Ukucaciswa | Akukho ukuhlangana kwezifo ezithathelwanayo nezinye izifo ezithathelwanayo ngaphandle kobubanzi bokutholakala kwekhithi yokuhlola, njenge-Treponema pallidum, i-Mycoplasma hominis, i-Mycoplasma genitalium, i-Herpes simplex virus type 1, i-Herpes simplex virus type 2, i-Candida albicans, njll. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-reagent yokukhipha enconyiwe: I-Pipette 1mL yesampula ezohlolwa ku-1.5mL ye-DNase/RNase-free centrifuge tube, i-centrifuge ku-12000rpm imizuzu emi-3, lahla i-supernatant bese ugcina i-precipitate. Engeza u-200µL we-saline evamile ku-precipitate ukuze uphinde uyimise. I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhipha kufanele kwenziwe ngokwemiyalelo yokusebenzisa. Ivolumu yesampula ekhishwe ingu-200µL, kanti ivolumu yokususa enconyiwe ingu-80µL.
Inketho 2.
I-reagent yokukhipha enconywayo: I-Nucleic Acid Extraction noma i-Purification Kit (YDP302). Ukukhipha kufanele kwenziwe ngokuqinile ngokwemiyalelo yokusebenzisa. Umthamo wokususa onconywayo u-80µL.
