I-Chlamydia Trachomatis, Neisyria Gonorrhoaae neTrichomonas Vaginalis
Igama lomkhiqizo
I-HWS-UR041 CHEMMYDIA TRACHYDIA TRACHOMISI, NEISSERIA GONORRRRHOEAE NE-Trichomonas Vaginalis nucleic acid ukutholwa kokutholwa (Fluorescence PCR)
I-Epidemiology
I-Chlamydia Trachomatis (CT) uhlobo lwe-prokaryotic microorganism oluhambisana ngokuqinile emangqamuzaneni e-eukaryotic. I-Chlamydia Trachomatis ihlukaniswe ngama-ak serotypes ngokuya ngendlela ye-serotype. Ukutheleleka kwamapheshana e-Urogenital kubangelwa kakhulu yi-trachoma biological varetros ehlukile ye-delotypes, futhi abesilisa babonakaliswa kakhulu njenge-urethritis, engakhululwa ngaphandle kokulashwa, kepha iningi labo liba yi-epidedymitis, i-Proctitis, njll.
Insungubezi
-Yenza | I-Chlamydia Trachomatis |
Ivupho | Neisreria gonorrhoaae |
Cy5 | I-Trichomonal Vaginitis |
VIC / Hex | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
Ukubeka | -18 ℃ |
Ishalofu-impilo | Izinyanga eziyi-12 |
Uhlobo lwe-Specimen | Isibeletho sowesifazane wesibeletho,Isitabane sowesifazane sowesifazane,I-urethral yabesilisa |
Ct | ≤38 |
CV | <5% |
Indlu edayisa insimbi | 400Amakhophi / ml |
Ukucacisa | Akukho okuphambana nokuphindaphindeka namanye ama-pathogens atheleleka nge-STD ngaphandle kwe-Treponema Palidum, i-MyCoplasma Hominis, i-Herpes Simplex Virus Virus hlobo 2, i-Alcida Alc, njll. |
Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
Recommended extraction reagent: Pipette 1mL of the sample to be tested to a 1.5mL of DNase/RNase-free centrifuge tube, centrifuge at 12000rpm for 3 minutes, discard the supernatant and keep the precipitate. Faka i-200μL ye-saline ejwayelekile ku-precipitate ukuze uphinde usebenze. I-Macro & Micro-Test General DNA / RNA KIT (HWTS-3019-50, HWTS-3019-52, HWTS-3019-48, HWS-3019-48, HWS-3019-46, HWS-3019-46) (okungasetshenziswa nge-macro & micro-avivinya i-othomathikhi ye-nucle (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuya ngomyalo wokusetshenziswa. Ivolumu yesampula ekhishwe i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-80μL.
Inketho 2.
I-reaglent enconyiwe ye-reagent: ukukhishwa kwe-nucleic acid noma i-arpication kit (yppp02). Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngomyalo wokusebenzisa. IVolumu Ye-Expeated Enconyiwe ingu-80μL.