I-chramydia eyomile ye-chlamydia trachomatis
Igama lomkhiqizo
I-HWTS-ur0032C / D-Frozeze-Omisiwe Chlamydia Trachomatis nucleic acid detection detection
I-Epidemiology
I-Chlamydia Trachomatis (CT) uhlobo lwe-prokaryotic microorganism oluhambisana ngokuqinile kumaseli we-eukaryotic[1]. I-Chlamydia Trachomatis ihlukaniswe ngama-ak serotypes ngokuya ngendlela ye-serotype. Ukutheleleka kwamapheshana e-Urogenital kubangelwa kakhulu yi-trachoma biological varetros ehlukile ye-dekotypes, futhi abesilisa babonakaliswa kakhulu njenge-urethritis, engakhululwa ngaphandle kokwelashwa, kepha iningi labo liba yi-epidedymitis, i-proctitis, njll[2]. Abesifazane bangenziwa nge-urethritis, i-cervicitis, njll., Kanye nezinkinga ezinkulu kakhulu ze-salpingitis[3].
Insungubezi
| -Yenza | I-Chlamydia Trachomatis (CT) |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤30 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | I-Swap ye-CEVICAL SWAB I-urethral yabesilisa Umchamo wesilisa |
| Tt | ≤28 |
| CV | ≤10.0% |
| Indlu edayisa insimbi | Amakhophi angama-400 / ml |
| Ukucacisa | Akukho okuphambana nokuphindaphindeka phakathi kwaleli khithi namanye ama-papitoogen okutheleleka okutheleleka kwe-genitorinatoriner , Escherichia Coli, Gardenerla Vaginalis, i-Alicomonas Albicans, i-Trichomas Vaginalis, i-Adenovacillus Crispatus, i-Adenovirus, i-cytomegalovirus, i-beta streptococcus, i-lactobacilfus castei ne-geane genomic DNA, njll. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Plus real-time PCCRECT SYPELY (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR ne-Biorad CFX Opus 96 Uhlelo lwe-PRCR lwangempela lwe-Real-time PCR Easy AMP-time fluorescence timescence tothermal detection templettem(I-HWTS-1600Isihlehlukene. |
Ukugeleza komsebenzi
Inketho 1.
I-Macro & Micro-Test Samputer Supder Reled Reagent (HWS-3005-8). Ukukhishwa kufanele kwenziwe ngokuqinile nge-IFU. Faka i-DNA ekhishwe isampula ukukhishwa kwesampula ku-buffent yokuphendula bese uvivinya ithuluzi ngqo, noma amasampula akhishwe kufanele agcinwe ku-2-8 ② mahhala amahora angama-24.
Inketho 2.
I-Macro & Micro-Test General DNA / RNA KIT (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-macro & micro-test automatic atholela i-nucleic acid extractor (HWS-3006C, HWS-3006B). Ukukhishwa kufanele kwenziwe ngokuqinile ngokuhambisana ne-IFU, futhi ivolumu yokuqedwa elinconyiwe liyi-80μL. I-DNA eyisampula ekhishwe yindlela ye-magnetic bead ifudunyezwa ku-95 ° C imizuzu emi-3 bese ngokushesha i-ice-yagezelwe imizuzu emi-2. Faka i-DNA esetshenzisiwe yesampula kwi-buffer yokuphendula bese uvivinya ithuluzi noma amasampula asetshenzisiwe kufanele agcinwe ngaphansi -18 ° C isikhathi esingaphezu kwezinyanga ezi-4. Inani lokuqandeka okuphindaphindiwe futhi lihlanza akufanele lidlule imijikelezo emi-4.
