I-Chlamydia Trachomatis eqandisiwe
Igama lomkhiqizo
HWTS-UR032C/D-Ikhithi yokutholwa kwe-Chlamydia Trachomatis Nucleic Acid eyomisiwe (Enzymatic Probe Isothermal Amplification)
I-Epidemiology
I-Chlamydia trachomatis (CT) iwuhlobo lwe-prokaryotic microorganism ene-parasitic eqinile kumaseli e-eukaryotic.[1].I-Chlamydia trachomatis ihlukaniswe ngama-serotypes e-AK ngokuya ngendlela ye-serotype.Izifo ze-Urogenital tract ngokuvamile zibangelwa i-trachoma biological variant DK serotypes, futhi abesilisa babonakaliswa kakhulu njenge-urethritis, engakhululeka ngaphandle kokwelashwa, kodwa iningi labo liba elingapheli, ngezikhathi ezithile lithuthuke, futhi lingahlanganiswa ne-epididymitis, i-proctitis, njll.[2].Abesifazane bangabangelwa i-urethritis, i-cervicitis, njll., kanye nezinkinga ezimbi kakhulu ze-salpingitis.[3].
Isiteshi
| FAM | I-Chlamydia trachomatis (CT) |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤30℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomlomo wesibeletho wesifazane I-swab ye-urethral yesilisa Umchamo wesilisa |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Amakhophi angu-400/mL |
| Ukucaciswa | akukho-reactivity phakathi kwaleli kit namanye amagciwane okutheleleka nge-genitourinary tract afana ne-Human papillomavirus type 16, i-Human papillomavirus type 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasmspilogenider , Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei kanye human genomic DNA, njll. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System kanye ne-BioRad CFX Opus 96 Real-Time PCR System I-Easy Amp Real-time Fluorescence Isothermal Detection System(I-HWTS-1600）. |
Ukugeleza komsebenzi
Inketho 1.
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ukukhishwa kufanele kwenziwe ngokuhambisana ne-IFU.Engeza isampula ye-DNA ekhishwe i-reagent yokukhishwa kwesampula kubhafa yokusabela futhi uhlole ithuluzi ngokuqondile, noma amasampuli akhishiwe kufanele agcinwe ku-2-8℃ isikhathi esingengaphezu kwamahora angu-24.
Inketho yesi-2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ukukhishwa kufanele kwenziwe ngokuhambisana ngokuqinile ne-IFU, futhi umthamo we-elution onconywayo ngu-80μL.Isampula ye-DNA ekhishwe ngendlela yobuhlalu obuzibuthe ishisisa ku-95°C imizuzu emi-3 bese igezwa eqhweni ngokushesha imizuzu emi-2.Engeza isampula ye-DNA ecutshunguliwe ku-bafa yokusabela futhi uhlole entweni noma amasampula asetshenziwe kufanele agcinwe ngaphansi kuka -18°C isikhathi esingeqi izinyanga ezi-4.Inani lokuqandisa okuphindaphindiwe nokuncibilika akufanele lidlule imijikelezo emi-4.
