I-Chlamydia Trachomatis eqandisiwe
Igama lomkhiqizo
HWTS-UR032C/D-Ikhithi yokutholwa kwe-Chlamydia Trachomatis Nucleic Acid eyomisiwe (Enzymatic Probe Isothermal Amplification)
I-Epidemiology
I-Chlamydia trachomatis (CT) iwuhlobo lwe-prokaryotic microorganism ene-parasitic eqinile kumaseli e-eukaryotic.[1]. I-Chlamydia trachomatis ihlukaniswe ngama-serotypes e-AK ngokuya ngendlela ye-serotype. Izifo ze-Urogenital tract ngokuvamile zibangelwa i-trachoma biological variant DK serotypes, futhi abesilisa babonakaliswa kakhulu njenge-urethritis, engakhululeka ngaphandle kokwelashwa, kodwa iningi labo liba elingapheli, ngezikhathi ezithile lithuthuke, futhi lingahlanganiswa ne-epididymitis, i-proctitis, njll.[2]. Abesifazane bangabangelwa i-urethritis, i-cervicitis, njll., kanye nezinkinga ezimbi kakhulu ze-salpingitis.[3].
Isiteshi
| FAM | I-Chlamydia trachomatis (CT) |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤30℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomlomo wesibeletho wesifazane I-swab ye-urethral yesilisa Umchamo wesilisa |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Amakhophi angu-400/mL |
| Ukucaciswa | akukho-reactivity phakathi kwaleli kit namanye amagciwane okutheleleka nge-genitourinary tract afana ne-Human papillomavirus type 16, i-Human papillomavirus type 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma, Staphylogenic epilogenider Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei kanye human genomic DNA, njll. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System kanye ne-BioRad CFX Opus 96 Real-Time PCR System I-Easy Amp Real-time Fluorescence Isothermal Detection System(I-HWTS-1600）. |
Ukugeleza komsebenzi
Inketho 1.
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Ukukhishwa kufanele kwenziwe ngokuhambisana ne-IFU. Engeza isampula ye-DNA ekhishwe i-reagent yokukhishwa kwesampula kubhafa yokusabela futhi uhlole ithuluzi ngokuqondile, noma amasampuli akhishiwe kufanele agcinwe ku-2-8℃ isikhathi esingengaphezu kwamahora angu-24.
Inketho yesi-2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhishwa kufanele kwenziwe ngokuhambisana ngokuqinile ne-IFU, futhi umthamo we-elution onconywayo ngu-80μL. Isampula ye-DNA ekhishwe ngendlela yobuhlalu obuzibuthe ishisisa ku-95°C imizuzu emi-3 bese igezwa eqhweni ngokushesha imizuzu emi-2. Engeza isampula ye-DNA ecutshunguliwe ku-bafa yokusabela futhi uhlole entweni noma amasampula asetshenziwe kufanele agcinwe ngaphansi kuka -18°C isikhathi esingeqi izinyanga ezi-4. Inani lokuqandisa okuphindaphindiwe nokuncibilika akufanele lidlule imijikelezo emi-4.
