I-Chlamydia Trachomatis eyomiswe ngeqhwa
Igama lomkhiqizo
I-HWTS-UR032C/D-Ikhithi Yokuthola I-Chlamydia Trachomatis Nucleic Acid Eyomisiwe Ngokuqandisa (I-Enzymatic Probe Isothermal Amplification)
I-Epidemiology
I-Chlamydia trachomatis (CT) uhlobo lwe-prokaryotic microorganism oluyi-parasitic kuphela kumaseli e-eukaryotic[1]I-Chlamydia trachomatis ihlukaniswe ngama-serotype e-AK ngokwendlela ye-serotype. Ukutheleleka kwe-Urogenital tract kubangelwa kakhulu yi-trachoma biological variant DK serotypes, kanti abesilisa bavame ukubonakala njenge-urethritis, engaqedwa ngaphandle kokwelashwa, kodwa iningi labo liba ngelapheki, liba libi ngezikhathi ezithile, futhi lingahlanganiswa ne-epididymitis, i-proctitis, njll.[2]Abesifazane bangabangelwa yi-urethritis, i-cervicitis, njll., kanye nezinkinga ezinkulu ze-salpingitis.[3].
Isiteshi
| I-FAM | I-Chlamydia trachomatis (CT) |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤30℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomlomo wesibeletho yowesifazane I-swab ye-urethra yabesilisa Umchamo wesilisa |
| Tt | ≤28 |
| CV | ≤10.0% |
| I-LoD | Amakhophi angu-400/mL |
| Ukucaciswa | Akukho ukuhlangana phakathi kwale khithi nezinye izifo ezibangelwa ukutheleleka kwe-genitourinary tract ezifana ne-Human papillomavirus uhlobo 16 oluyingozi kakhulu, i-Human papillomavirus uhlobo 18, i-Herpes simplex virus type Ⅱ, i-Treponema pallidum, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium, i-Staphylococcus epidermidis, i-Escherichia coli, i-Gardnerella vaginalis, i-Candida albicans, i-Trichomonas vaginalis, i-Lactobacillus crispatus, i-Adenovirus, i-Cytomegalovirus, i-Beta Streptococcus, i-Human immunodeficiency virus, i-Lactobacillus casei kanye ne-human genomic DNA, njll. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR kanye nohlelo lwe-BioRad CFX Opus 96 Real-Time PCR Uhlelo Lokuthola Isothermal Lwe-Easy Amp Real-time Fluorescence(I-1965)I-HWTS-1600）. |
Ukugeleza Komsebenzi
Inketho 1.
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Ukukhishwa kufanele kwenziwe ngokuhambisana ne-IFU. Faka i-DNA yesampula ekhishwe yi-release release release reagent ku-reaction buffer bese uhlola ithuluzi ngqo, noma amasampula akhishwe kufanele agcinwe ku-2-8℃ isikhathi esingaphezu kwamahora angama-24.
Inketho 2.
Ikhithi ye-DNA/RNA Jikelele ye-Macro kanye ne-Micro-Test (HWTS-301)7-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhishwa kufanele kwenziwe ngokuhambisana ne-IFU, futhi ivolumu yokuhlanza enconywayo ingu-80μL. I-DNA yesampula ekhishwe ngendlela ye-magnetic bead ishiswa ku-95°C imizuzu emi-3 bese ifakwa eqhweni ngokushesha imizuzu emi-2. Faka i-DNA yesampula ecutshunguliwe ku-reaction buffer bese uhlola ithuluzi noma amasampula acutshunguliwe kufanele agcinwe ngaphansi kuka-18°C isikhathi esingaphezu kwezinyanga ezi-4. Inani lokuqandisa nokuncibilikisa okuphindaphindiwe akufanele lidlule imijikelezo emi-4.
