Izifo Eziyisithupha Zokuphefumula Eziyomisiwe Ngokuqandisa I-Nucleic Acid

Izifo Eziyisithupha Zokuphefumula Eziyomisiwe Ngokuqandisa Isithombe Esivelele Se-Nucleic Acid

Incazelo emfushane:

Lo mkhiqizo usetshenziselwa ukuthola igciwane lokuphefumula elihambisanayo (RSV) ngaphakathi kwe-vitro, i-adenovirus (Adv), i-human metapneumovirus (hMPV), i-rhinovirus (Rhv), igciwane le-parainfluenza uhlobo I/II/III (PIVI/II/III) kanye ne-Mycoplasma pneumoniae (MP) nucleic acid kumasampula e-human nasopharyngeal swab.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola Amagciwane Okuphefumula Ayisithupha Eyomisiwe Nge-HWTS-RT192 (i-Fluorescence PCR)

I-Epidemiology

Ukutheleleka ngomgudu wokuphefumula uhlobo oluvame kakhulu lwesifo sabantu, olungavela kunoma yiluphi ubulili, ubudala kanye nesifunda, futhi lungenye yezimbangela ezibaluleke kakhulu zokugula nokufa emhlabeni [1]. Izifo ezivame kakhulu zokuphefumula zifaka phakathi igciwane lokuphefumula elihambisanayo, i-adenovirus, i-human metapneumovirus, i-rhinovirus, igciwane le-parainfluenza (I/II/III) kanye ne-mycoplasma pneumoniae, njll. [2,3]. Izimpawu zomtholampilo nezimpawu ezibangelwa ukutheleleka ngomgudu wokuphefumula ziyafana, kodwa ukutheleleka okubangelwa amagciwane ahlukene kunezindlela ezahlukene zokwelapha, imiphumela yokwelapha kanye nendlela isifo esiqhubeka ngayo [4,5]. Njengamanje, izindlela eziyinhloko zokuthola amagciwane okuphefumula elabhorethri zifaka: ukuhlukaniswa kwegciwane, ukutholwa kwe-antigen kanye nokutholwa kwe-nucleic acid, njll. Le khithi ithola futhi ihlonze ama-virtual nucleic acid athile kubantu abanezimpawu nezimpawu zokutheleleka kokuphefumula, kanye neminye imiphumela yezokwelapha neyelabhorethri ukusiza ekuxilongweni kokutheleleka ngegciwane kokuphefumula.

Amapharamitha Obuchwepheshe

Isitoreji	2-28℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	I-nasopharyngeal swab
Ct	I-RSV,Adv,hMPV,Rhv,PIV,MP Ct≤35
I-LoD	Amakhophi angu-200/mL
Ukucaciswa	Ukusabela okuxubile: Akukho ukusabela okuxubile phakathi kwekhithi negciwane leBoca, i-Cytomegalovirus, igciwane le-Epstein-Barr, igciwane le-Herpes simplex, igciwane le-varicella zoster, igciwane le-Mumps, igciwane le-Enterovirus, igciwane le-measles, igciwane le-human coronavirus, i-SARS Coronavirus, i-MERS Coronavirus, i-Rotavirus, i-Norovirus, i-Chlamydia pneumoniae, i-Streptococcus pneumoniae, i-Klebsiella pneumoniae, i-Streptococcus pyogenes, i-Legionella, i-Pneumospora, i-Haemophilus influenzae, i-Bacillus pertussis, i-Staphylococcus aureus, i-Mycobacterium tuberculosis, i-gonococcus, i-Candida albicans, i-Candida glabra, i-Aspergillus fumigatus, i-Cryptococcus neoformans, i-Streptococcus salivarius, i-Moraxella catarrh, i-Lactobacillus, i-Corynebacterium, i-human genomic DNA.
Izinsimbi Ezisebenzayo	Kusebenza ku-reagent yokuhlola yohlobo lwe-I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.). Kusebenza ku-reagent yokuhlola yohlobo lwesibili: I-Eudemon^TMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Ukugeleza Komsebenzi

I-PCR evamile

I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. iyanconywa ekukhishweni kwesampula futhi izinyathelo ezilandelayo kufanele zenziwe ngokuhambisana ne-IFU ye-Kit.