I-Gardnerella Vaginalis Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-UR042-Gardnerella Vaginalis Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Imbangela evame kakhulu ye-vaginitis kwabesifazane yi-bacterial vaginosis, kanti igciwane elibalulekile le-bacterial vaginosis yi-Gardnerella vaginalis. I-Gardnerella vaginalis (GV) iyi-pathogen engabangeli izifo uma ikhona ngamanani amancane. Kodwa-ke, lapho i-bacterial eyinhloko yesitho sangasese sowesifazane i-Lactobacilli incishisiwe noma iqedwa, okubangela ukungalingani endaweni yesitho sangasese sowesifazane, i-Gardnerella vaginalis iyanda ngamanani amakhulu, okuholela ku-bacterial vaginosis. Ngesikhathi esifanayo, ezinye izifo (njenge-Candida, i-Neisseria gonorrhoeae, i-Chlamydia trachomatis, njll.) zingase zihlasele umzimba womuntu, zibangele i-mixed vaginitis kanye ne-cervicitis. Uma i-vaginitis kanye ne-cervicitis zingatholakali futhi zingalashwa ngendlela efanele futhi ngesikhathi esifanele, kungase kube nokutheleleka okukhuphukayo yizifo ezithinta i-mucosa yomgudu wokuzala, okuholela kalula ekuthelelekeni komgudu wokuzala ophezulu njenge-endometritis, i-salpingitis, i-tubo-ovarian abscess (TOA), kanye ne-pelvic peritonitis, okungaholela ezinkingeni ezinkulu njengokungazali, ukukhulelwa kwe-ectopic ngisho nemiphumela emibi yokukhulelwa.
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | ama-swabs esitho sangasese sowesifazane, ama-swabs esitho sangasese sowesifazane, i-swab yesitho sangasese sowesifazane |
| Ct | ≤38 |
| CV | ＜5.0% |
| I-LoD | Amakhophi angu-400/mL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I:Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-EudemonTM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.
