Uhlobo lweBraf lwe-Human Brain v600e
Igama lomkhiqizo
I-HWTS-TM007-Human Braf Gene v600e Detentionation Detection Kit (Fluorescence PCR)
Isitifiketi
Ce / tfda
I-Epidemiology
Kutholwe izinhlobo ezingaphezu kuka-30 zokuguqulwa kwe-braf kwe-braf, kwazo cishe ama-90% atholakala ku-exon 15, lapho ukuguqulwa kwe-v600e kubhekwa njenge-mutation ejwayelekile, okungukuthi, i-thymine (t) esikhungweni se-1799 esivulelekile i-adenine (a), okuholela ekushintshelweni kwe-valene (v) endaweni eyisisekelo engu-600 nge-glutamic acid (e) kumkhiqizo wamaprotheni. Izakhi zofuzo ze-braf zivame ukutholakala kuthululo olubi njenge-melanoma, umdlavuza we-colorectal, umdlavuza we-thyroid, nomdlavuza wamaphaphu. Ukuqonda ukuguqulwa kwe-gene gene sekuyisidingo sokuhlola izidakamizwa ze-EGFR-TKIs kanye ne-braf gene gene aution-okuhlosiwe ekwelashweni kwezidakamizwa okuhlosiwe kwemitholampilo ezigulini ezingasizakala.
Insungubezi
| -Yenza | V600e meation, ukulawulwa kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | Amasampula we-paraffed-eshunye ashunyekiwe we-paraffed |
| CV | <5.0% |
| Ct | ≤38 |
| Indlu edayisa insimbi | Sebenzisa amakhithi ukuthola ukulawulwa kwekhwalithi ye-Lod ehambisanayo. a) Ngaphansi kwesendlalelo se-3ng / μL yasendle, i-1% yokuguqulwa kwezilinganiso ingatholwa ku-reacler buffly ngokuqinile; b) Ngaphansi kwe-1% yokuguqulwa kwezilinganiso, ukuguqulwa kwe-1 × 103Amakhophi / ml esibonelweni sohlobo lwasendle se-1 × 105Amakhophi / ml angatholwa kangako esikhwameni sokuphendula; c) I-IC reaction buffer ingabona ikhwalithi ephansi yokuthola umkhawulo wekhwalithi ye-SW3 yenkampani yokulawula kwangaphakathi. |
| Izinsimbi ezisebenzayo: | Kusetshenziswe ama-biosystems 7500 real-time PCREMSKusetshenziswe ama-biosystem 7300 real-time pcr Amasistimu, I-QuantStudio® 5 Systems real-time PCREMS I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-Biorad CFX96 Uhlelo lwe-Real-Time PCR |
Ukugeleza komsebenzi
I-reagents yokukhishwa enconyiwe: I-Qiagen's QA FFPE FFPE izicubu ze-FFPE (56404), izicubu ezishumekiwe ze-paraffin i-DNA ngokushesha ukukhishwa Kit (DPS330) Kwenziwe yiTiangen Biotech (Beijing) Co, Ltd.
