Ukuguqulwa kweGene V600E yomuntu
Igama lomkhiqizo
Ikhithi Yokuthola Ukuguquka Kwe-HWTS-TM007-Human BRAF Gene V600E (i-Fluorescence PCR)
Isitifiketi
I-CE/TFDA
I-Epidemiology
Kutholakale izinhlobo ezingaphezu kuka-30 zokuguqulwa kwe-BRAF, okungaba ngu-90% kuzo ezitholakala ku-exon 15, lapho ukuguqulwa kwe-V600E kubhekwa njengokuguqulwa okuvamile kakhulu, okungukuthi, i-thymine(T) endaweni engu-1799 ku-exon 15 iguqulwa ibe yi-adenine (A), okuholela ekufakweni kwe-valine (V) endaweni engu-600 yi-glutamic acid (E) kumkhiqizo weprotheni. Ukuguqulwa kwe-BRAF kuvame ukutholakala ezimila ezinobungozi ezifana ne-melanoma, umdlavuza we-colorectal, umdlavuza we-thyroid, kanye nomdlavuza wamaphaphu. Ukuqonda ukuguqulwa kwe-BRAF gene sekuyisidingo sokuhlola i-EGFR-TKIs kanye nemithi eqondiswe ekuguqulweni kwezakhi zofuzo ze-BRAF ekwelashweni kwemithi eqondiswe emtholampilo yeziguli ezingazuza.
Isiteshi
| I-FAM | Ukuguqulwa kwe-V600E, ukulawula kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | amasampula ezicubu ezibangelwa izifo ezifakwe iparafini |
| CV | ＜5.0% |
| Ct | ≤38 |
| I-LoD | Sebenzisa amakhithi ukuthola ukulawulwa kwekhwalithi ye-LoD okuhambisanayo. a) Ngaphansi kwesizinda se-wild-type esingu-3ng/μL, izinga lokuguquka elingu-1% lingatholakala ku-reaction buffer ngokuzinzile; b) ngaphansi kwesilinganiso sokuguquka esingu-1%, ukuguqulwa okungu-1×103Amakhophi/mL ngemuva kohlobo lwasendle lwe-1×105Amakhophi/mL angatholakala ngokuzinzile ku-reaction buffer; c) i-IC Reaction Buffer ingathola umkhawulo ophansi kakhulu wokulawula ikhwalithi ye-SW3 yokulawula kwangaphakathi kwenkampani. |
| Izinsimbi Ezisebenzayo: | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-TimeI-Applied Biosystems 7300 I-PCR Yesikhathi Sangempela Izinhlelo, i-QuantStudio® 5 Izinhlelo ze-PCR Zesikhathi Sangempela Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo lwe-BioRad CFX96 Real-Time PCR |
Ukugeleza Komsebenzi
Ama-reagent okukhipha anconyiwe: I-QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), I-Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) eyenziwe yi-Tiangen Biotech(Beijing) Co., Ltd.
