Ikhithi Yokuthola I-Human Leukocyte Antigen B27 Nucleic Acid

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola i-DNA esezingeni eliphezulu kuma-antigen e-human leukocyte antigen i-HLA-B*2702, i-HLA-B*2704 kanye ne-HLA-B*2705.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola I-Human Leukocyte Antigen B27 Nucleic Acid (i-Fluorescence PCR) ye-HWTS-GE011 Human Leukocyte Antigen B27 Nucleic Acid (i-Fluorescence PCR)

I-Epidemiology

I-Ankylosing spondylitis (AS) yisifo sokuvuvukala esingapheli esihlasela umgogodla futhi singase sihilele amalunga e-sacroiliac kanye namalunga azungezile ngamazinga ahlukahlukene. Kuye kwavezwa ukuthi i-AS ibonisa ukuhlangana okusobala komndeni futhi ihlobene eduze ne-human leukocyte antigen HLA-B27. Kubantu, izinhlobo ezingaphezu kuka-70 zezinhlobo ze-HLA-B27 zitholakale futhi zatholakala, futhi kuzo, i-HLA-B*2702, i-HLA-B*2704 kanye ne-HLA-B*2705 yizinhlobo ezivame kakhulu ezihlobene nalesi sifo. EShayina, eSingapore, eJapan kanye naseTaiwan esifundeni saseShayina, uhlobo oluvame kakhulu lwe-HLA-B27 yi-HLA-B*2704, ebalelwa cishe ku-54%, ilandelwe yi-HLA-B*2705, ebalelwa cishe ku-41%. Le khithi ingathola i-DNA ngezinhlobo ezincane ze-HLA-B*2702, i-HLA-B*2704 kanye ne-HLA-B*2705, kodwa ayizihlukanisi.

Isiteshi

I-FAM	I-HLA-B27
I-ROX	Ukulawula Kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	Uketshezi: ≤-18℃
Isikhathi sokuphelelwa yisikhathi	Uketshezi: Izinyanga ezingu-18
Uhlobo Lwesifanekiso	amasampula egazi lonke
Ct	≤40
CV	≤5.0%
I-LoD	1ng/μL
Ukucaciswa	Imiphumela yokuhlolwa etholwe yile kit ngeke ithintwe yi-hemoglobin (<800g/L), i-bilirubin (<700μmol/L), kanye nama-lipids egazini/ama-triglycerides (<7mmol/L) egazini.
Izinsimbi Ezisebenzayo	Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-Biosystems ezisetshenzisiwe ze-StepOne Real-Time PCR I-LightCycler^®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo lwe-Agilent-Stratagene Mx3000P Q-PCR