I-leukocyte yabantu i-antigen b27 khit dection ye-nucleic acid

I-Human Leukocyte Antigen B27 Kit Detection Detection Kit efakiwe

Incazelo emfushane:

Le khithi isetshenziselwa ukutholwa kwe-DNA kwe-DNA e-Suman Leukocyte antigen subtypes hla-b * 2702, HLA-B * 2704 neHLA-B * 2704.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-GE011 Human Leukocyte Antigen B27 Kit Detection Detection (Fluorescence PCR)

I-Epidemiology

I-Ankylosing Spondylitis (AS) yisifo esingamahlalakhona sokuvuvukala esithuthukayo esiphazamisa kakhulu umgogodla futhi singafaka amalunga we-warlwaliac kanye namalunga azungezile emagcekeni ahlukahlukene. Kuveziwe ukuthi njengoba kukhombisa ukuhlanganiswa okusobala komndeni futhi kuhlobene kakhulu ne-leukocyte yabantu antigen hla-b27. Ebuntwini, kutholakale izinhlobo ezingaphezu kuka-70 zezinhlobo zama-HLA-B2TY, futhi kuzo, kwathi, kwathi, HLA-B * 2702, HLA-B * 2704 noHla-B * 2705 kanti i-HLA-B * 2705 yilezi eziphakeme kakhulu ezihlobene nalesi sifo. E-China, eSingapore, eJapan naseTaiwan esifundeni saseChina, i-subtype evame kakhulu ye-HLA-B27 yi-HLA-B * 2704, ibala cishe ama-54%, ilandelwa yi-HLA-B *70, elandela cishe ama-41%. Le khithi ingathola i-DNA kwi-subtypes hla-b * 2702, hla-b * 2704 ne-hla-b * 2705, kepha ayiyehlukanisi komunye nomunye.

Insungubezi

-Yenza	Hla-b27
Ivupho	Ukulawulwa Kwangaphakathi

Amapharamitha Ezobuchwepheshe

Ukubeka	Uketshezi: ≤-18 ℃
Ishalofu-impilo	Uketshezi: izinyanga eziyi-18
Uhlobo lwe-Specimen	amasampula egazi aphelele
Ct	≤40
CV	≤5.0%
Indlu edayisa insimbi	1ng / μl
Ukucacisa	Imiphumela yokuhlolwa etholwe yile khithi ngeke ithintwe yi-hemoglobin (<800g / L), i-Bilirubin (<700μmol / l), kanye ne-lipids yegazi / i-triglycerides (<7mmol / l) egazini.
Izinsimbi ezisebenzayo	Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystems stepone real-time PCREMS I-Lightcycler^®480 Uhlelo lwe-PCR lwe-Real-time real Isistimu ye-Agilent-Stratagene MX3000p Q-PCR