I-Human Papillomavirus (Izinhlobo ezingu-28) Ukuhlolwa kwezakhi zofuzo
Igama lomkhiqizo
I-HWTS-CC013-I-Human Papillomavirus (Izinhlobo ezingu-28) Ikhithi Yokuthola Ufuzo (i-Fluorescence PCR)
I-HWTS-CC016A-I-Human Papillomavirus eyomisiwe ngokuqandisa (Izinhlobo ezingu-28) Ikhithi Yokuthola Ufuzo (i-Fluorescence PCR)
I-Epidemiology
Umdlavuza womlomo wesibeletho ungenye yezimila eziyingozi ezivame kakhulu ezithweni zokuzala zabesifazane. Izifundo zangaphambilini zikhombisile ukuthi ukutheleleka okuqhubekayo kanye nokutheleleka okuningi kwe-human papillomavirus kungenye yezimbangela ezibalulekile zomdlavuza womlomo wesibeletho. Njengamanje, kusenokuntuleka kwezindlela zokwelapha ezisebenzayo eziqashelwayo ze-HPV, ngakho-ke ukutholakala kusenesikhathi kanye nokuvimbela kusenesikhathi i-HPV yomlomo wesibeletho kuyizihluthulelo zokuvimba umdlavuza. Ukusungula indlela elula, eqondile futhi esheshayo yokuxilonga i-etiological kubaluleke kakhulu ekuxilongweni komdlavuza womlomo wesibeletho emtholampilo.
Isiteshi
| I-Reaction buffer | I-FAM | I-VIC/HEX | I-ROX | CY5 |
| I-HPV Genotyping Reaction Buffer 1 | 16 | 18 | / | Ukulawula kwangaphakathi |
| I-HPV Genotyping Reaction Buffer 2 | 56 | / | 31 | Ukulawula kwangaphakathi |
| I-HPV Genotyping Reaction Buffer 3 | 58 | 33 | 66 | 35 |
| I-HPV Genotyping Reaction Buffer 4 | 53 | 51 | 52 | 45 |
| I-HPV Genotyping Reaction Buffer 5 | 73 | 59 | 39 | 68 |
| I-HPV Genotyping Reaction Buffer 6 | 6 | 11 | 83 | 54 |
| I-HPV Genotyping Reaction Buffer 7 | 26 | 44 | 61 | 81 |
| I-HPV Genotyping Reaction Buffer 8 | 40 | 43 | 42 | 82 |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-Swab Yesibeletho, I-Swab Yesitho Sangasese Sowesifazane, Umchamo |
| Ct | ≤28 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-300/mL |
| ukucaciswa | Yonke imiphumela iba mibi uma ikhithi isetshenziselwa ukuthola amasampula angacacile anemiphumela ehambisana nayo, okuhlanganisa i-ureaplasma urealyticum, i-chlamydia trachomatis yendlela yokuzala, i-candida albicans, i-neisseria gonorrhoeae, i-trichomonas vaginalis, isikhunta, i-gardnerella kanye nezinye izinhlobo ze-HPV ezingamboziwe yikhithi. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Izinhlelo ze-PCR zesikhathi sangempela ezingu-480 Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Sample Release Release Release Reagent (HWTS-3005-8)
Inketho 2.
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)
