HWTS-RT201A/B/C/D-RSV/ADV Ag Ukuhlolwa Okusheshayo

Ukuhlolwa Okusheshayo kwe-HWTS-RT201A/B/C/D-RSV/ADV Ag Isithombe Esifakiwe

Incazelo emfushane:

Le khithi yenzelwe ukuthola ama-antigen e-Respiratory Syncytial Virus kanye ne-Adenovirus ngaphakathi kwe-vitro ngamasampula e-nasal swabs avela kubasolwa ngokutheleleka zingakapheli izinsuku eziyi-7 kusukela izimpawu ziqalile kanye nokuxhumana okuseduze ekuxilongweni okuhlukile kokutheleleka kwendlela yokuphefumula yi-Respiratory Syncytial Virus kanye ne-Adenovirus. Imiphumela yokuhlolwa akufanele isetshenziswe njengesisekelo sodwa sesinqumo somtholampilo sokuxilongwa nokwelashwa. Ukuxilongwa okwengeziwe kwezokwelapha kufanele kuqinisekiswe ngezindlela zokuhlola ezihlukile kanye neminye imiphumela yezokwelapha.

Ikhithi yayiwukuhlolwa kwe-in vitro kuphela futhi ayizenzakalelayo.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

HWTS-RT201A/B/C/D-RSV/ADV Ag Ukuhlolwa Okusheshayo

Ukuvunyelwa Komkhiqizo

CE3018 (IVDR)

Izimiso zokuhlola

Ikhithi isebenzisa ubuchwepheshe be-immunochromatography ngendlela yesandwich ye-double-antibodyukuthola ama-antigen e-Respiratory Syncytial Virus kanye ne-Adenovirus. Umugqa wokuhlolayagcotshwa ngama-antibodies athile ukuze yakhe imigqa yokuhlola ye-pathogen eqondiwe.Ngesikhathi sokuhlolwa, isampula ecutshungulwayo ezohlolwa ingezwa emthonjeni wesampula womsele.Amakhasethi okuhlola. Uma isampula ezohlolwa iqukethe igciwane eliqondiwe kuukuhlushwa okuphezulu kune-LoD, i-antigen eqondiwe kanye nethagethi enelebula ehambisanayoi-antibody izokwakha i-reaction compound. Ngaphansi kwesenzo se-chromatography, i-I-reaction compound iqhubekela phambili nge-nitrocellulose membrane, i-agglutinates ku-umugqa wokuhlola bese ekugcineni kwakha ibhande elibomvu, okubonisa ukuthi kukhona ukuhlolwa okuhambisanayoigciwane. Ngokuphambene nalokho, uma isampula ingenayo igciwane eliqondiwe nomaUkuhlushwa kwe-antigen kuphansi kune-LoD, akukho bhande elibomvu elizovela emgqeni wokuhlola,okubonisa ukuthi kukhona igciwane elihambisanayo elibangela ukungasebenzi kahle. Kungakhathaliseki ukuthi kukhona yini igciwane elibangela ukungasebenzi kahleUma i-pathogen ihlosiwe kusampula, kufanele kube khona ibhendi elibomvu emgqeni wokulawula ikhwalithi (C),okusebenza njengokulawula kwangaphakathi ukuqapha inqubo ye-chromatography kanyeikhithi yokuhlola iyasebenza.