Igciwane le-Influenza A/ Igciwane le-Influenza B
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid ye-HWTS-RT174-Influenza A Virus/Influenza B Virus (i-Fluorescence PCR)
I-Epidemiology
Ngokusekelwe kumehluko we-antigenic phakathi kwe-NP gene kanye ne-M gene, amagciwane omkhuhlane angahlukaniswa ngezinhlobo ezine: igciwane le-influenza A (IFV A), igciwane le-influenza B (IFV B), igciwane le-influenza C (IFV C) kanye negciwane le-influenza D (IFV D)[1]. Igciwane le-Influenza A linabathwali abaningi kanye nama-serotype ayinkimbinkimbi, futhi lingathola ikhono lokusabalala kubo bonke abathwali ngokusebenzisa ukuhlanganiswa kwezakhi zofuzo kanye nokuguqulwa okuguquguqukayo. Abantu abanakho ukuzivikela okuhlala isikhathi eside egciwaneni le-influenza A, ngakho-ke abantu babo bonke ubudala ngokuvamile basengozini. Igciwane le-Influenza A liyigciwane eliyinhloko elibangela ubhubhane lwe-influenza[2]. Igciwane le-Influenza B livame kakhulu endaweni encane futhi okwamanje alinazo izinhlobo ezincane. Eziyinhloko ezibangela ukutheleleka kwabantu yi-B/Yamagata lineage noma i-B/Victoria lineage. Phakathi kwamacala aqinisekisiwe omkhuhlane emazweni angu-15 esifundeni sase-Asia-Pacific njalo ngenyanga, izinga eliqinisekisiwe legciwane le-influenza B lingu-0-92%[3]Ngokungafani negciwane le-influenza A, amaqembu athile njengezingane kanye nabantu asebekhulile asengozini yokuthola igciwane le-influenza B futhi asengozini yokuba nezinkinga, okubeka umthwalo omkhulu emphakathini kunegciwane le-influenza A.[4].
Isiteshi
| I-FAM | i-asidi ye-nucleic ye-MP |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isampula ye-oropharyngeal swab |
| Ct | Umkhuhlane A, Umkhuhlane BCt≤35 |
| CV | <5.0% |
| I-LoD | I-Flu A kanye ne-Flu Bkonke kungamakhophi angu-200/mL |
| Ukucaciswa | Ukusabela okuxubile: Akukho ukusabela okuxubile phakathi kwekhithi ne-Bocavirus, i-rhinovirus, i-cytomegalovirus, igciwane lokuphefumula elihambisanayo, igciwane le-parainfluenza, igciwane le-Epstein-Barr, igciwane le-herpes simplex, igciwane le-Varicella-zoster, igciwane le-mumps, igciwane le-enterovirus, igciwane le-measles, igciwane le-human metapneumovirus, i-adenovirus, igciwane le-human coronavirus, i-novel coronavirus, i-SARS coronavirus, i-MERS coronavirus, i-rotavirus, i-norovirus, i-Chlamydia pneumoniae, i-Mycoplasma pneumoniae, i-Streptococcus pneumoniae, i-Klebsiella pneumoniae, i-Streptococcus pyogenes, i-Legionella, i-Pneumocystis carinii, i-Haemophilus influenzae, i-Bordetella pertussis, i-Staphylococcus aureus, i-Mycobacterium tuberculosis, i-Neisseria gonorrhoeae, i-Candida albicans, i-Candida glabrata, i-Aspergillus fumigatus, i-Cryptococcus neoformans, i-Streptococcus salivarius, i-Moraxella catarrhalis, i-Lactobacillus, i-Corynebacterium, kanye i-DNA yezakhi zofuzo zomuntu. Ukuhlolwa kokuphazamiseka: Khetha i-mucin (60 mg/mL), igazi lomuntu (50%), i-phenylephrine (2mg/mL), i-oxymetazoline (2mg/mL), i-sodium chloride (20mg/mL) ene-preservative engu-5%, i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide (20μg/mL), i-triamcinolone acetonide (2mg/mL), i-budesonide (1mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-benzocaine (10%), i-menthol (10%), i-zanamivir (20mg/mL), i-peramivir (1mg/mL), i-mupirocin (20mg/mL), i-tobramycin (0.6mg/mL), i-oseltamivir (60ng/mL), i-ribavirin (10mg/L) ukuze kuhlolwe ukuphazamiseka, futhi imiphumela ikhombisa ukuthi izinto eziphazamisayo ekugxilweni okungenhla aziphazamisi ukutholakala kwekhithi. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.Kunconywa ukukhishwa kwesampula kanyeizinyathelo ezilandelayo kufanele zibeukuziphathakulandelwe ngokuqinile yi-IFUyeKithi.
