Umkhuhlane a virus / umkhuhlane b virus
Igama lomkhiqizo
I-HWTS-RT174-Umkhuhlane I-Virus / Umkhuhlane B Virus nucleic acid detection detection detection detection
I-Epidemiology
Ngokusekelwe omehluko we-antigenic phakathi kwe-NP Gene no-M Gene, ama-Mfuthi afushani angahlukaniswa ngezinhlobo ezine: umkhuhlane wegciwane (iFV B), umkhuhlane wegciwane (i-IFV D)[1]. Umkhuhlane Igciwane linamabutho amaningi nama-serotypes ayinkimbinkimbi, futhi angathola amandla okusabalalisa kuwo wonke ama-hosteres ngokubuyiselwa kofuzo kanye nokuguqulwa kwezakhi zofuzo. Abantu abaswele ukungavikeleki okuhlala njalo emkhunjini, ngakho abantu bayo yonke iminyaka ngokuvamile bathambekele. Umkhuhlane Igciwane yi-pathogen enkulu ebangela umkhuhlane pandemics[2]. Igciwane le-Logeenza B ligcwele kakhulu endaweni encane futhi njengamanje alinawo ama-subtypes. Okuphambili okubangela ukutheleleka komuntu yi-B / Yamagata Lineage noma uhlu lwe-B / Victoria. Phakathi kwamacala aqinisekisiwe omkhuhlane emazweni ayi-15 esifundeni sase-Asia-Pacific njalo ngenyanga, inani eliqinisekisiwe le-Logeenza B Virus ingu-0-92%[3]. Ngokungafani nomkhuhlane leli gciwane, amaqembu athile afana nezingane nabantu asebekhulile bathambekele ekutholeni umkhuhlane B.[4].
Insungubezi
| -Yenza | I-MP nucleic acid |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Isampula ye-Oropharyngengeal Swap |
| Ct | Umkhuhlane a, umkhuhlane bI-CT≤35 |
| CV | <5.0% |
| Indlu edayisa insimbi | Umkhuhlane a nomkhuhlane bwonke ama-200copies / ml |
| Ukucacisa | Ukuwela kabusha Ukuqalisa , i-adenovirus, umuntu ICoronavirus, inoveli coronavirus, ama-coronavirus, ama-coronavirus, i-rotavirus, norovirus, i-stcoplasma pneumoniae, i-strepplasma pyumoniae, i-streptococcus pyumoniae, i-streptococcus pyumoniae, I-Haemophilus Umkhuhlane, i-Bordetella Pertuss, Staphylococcus Tuberculosis, Neisperia Glabrhoae, i-AsperIDA Glabrata, i-aspergillus fumigatus, i-cryptococcus neoformans, i-streptococcus neoformans, i-streptococcus neoformalis, e-moraxella catarralis, I-Lacompacillus, i-corybacteracteracterium, ne-DNA yomuntu genomic. Ukuhlolwa kokuphazanyiswa: Khetha i-mucin (60 mg / ml), igazi lomuntu (50%), Phenylephrine (2mg / ml), i-sodium chloride (20mg / ml) ), Dexamethasone (20mg / ml), Flunesisolide (20μg / ml), i-Triamcinolone Acetonide (2mg / ML), i-Budesonide (1mg / ml), i-MomeTasone (2mg / ml), i-fluticasone (2mg / ml), i-anzocaine (10% (10%), i-menl (10%), Zanamivir (20mg / ml), i-peramivir (1mg / ml), i-mupirocin (20mg / ml), i-tobramycin (0.6mg / ml), i-iSeltAmivir (60mg / ml), i-ribavirin (10mg / l) ukuze kuhlolwe izivivinyo, futhi imiphumela ikhombisa lokho Izinto eziphazamisayo ekugxileni okungenhla aziphazamisi ukutholwa kwekhithi. |
| Izinsimbi ezisebenzayo | SLAN-96P real-time PCRS Systems Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-Macro & Micro-Toust Viral DNA / RNA KIT (HWTS-3017) (engasetshenziswa nge-macro & micro-test automatic extractor ye-nucleic acid (HWS-3006c, i-HWS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd.kunconyelwe ukukhishwa kwesampula kanyeIzinyathelo ezilandelayo kufanele zibeincovui-ted ngokuqinile ngokuhambisana ne-IFUyekhithi.
