Umkhuhlane A Virus Universal/H1/H3
Igama lomkhiqizo
I-HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Mycoplasma pneumoniae (MP) incane kakhulu i-prokaryotic microorganism enesakhiwo seseli futhi alukho udonga lweseli phakathi kwamagciwane namagciwane.I-MP ikakhulukazi ibangela izifo zemigudu yokuphefumula kubantu, ikakhulukazi ezinganeni nasebancane.I-MP ingabangela i-Mycoplasma hominis pneumonia, izifo zokuphefumula ezinganeni kanye ne-atypical pneumonia.Izimpawu zomtholampilo zihlukene, ikakhulukazi ukukhwehlela okubi kakhulu, imfiva, ukugodola, ikhanda elibuhlungu, umphimbo obuhlungu, ukutheleleka komgudu wokuphefumula ongenhla kanye ne-bronchopneumonia yizona ezivame kakhulu.Ezinye iziguli zingase zibe nenyumoniya eyingozi ngenxa yokutheleleka kwemigudu yokuphefumula ephezulu, futhi ukucindezeleka okukhulu kokuphefumula noma ngisho nokufa kungase kwenzeke.I-MP ingenye yamagciwane avamile futhi abalulekile ku-pneumonia etholwe ngumphakathi (CAP), ebalwa ku-10% -30% we-CAP, futhi ingxenye ingakhula izikhathi ezingu-3-5 lapho i-MP idlangile.Eminyakeni yamuva, ingxenye ye-MP ku-CAP pathogens iye yanda kancane kancane.Izigameko zokutheleleka nge-Mycoplasma pneumoniae ziye zanda, futhi ngenxa yokubonakala kwayo okungaqondile emitholampilo, kulula ukudideka nemikhuhlane ebangelwa amagciwane kanye negciwane.Ngakho-ke, ukutholwa kusenesikhathi elabhorethri kubaluleke kakhulu ekuxilongweni nasekwelashweni.
Isiteshi
| FAM | influenza Uhlobo lwe-universal virus nucleic acid |
| VIC/HEX | umkhuhlane A H1 uhlobo lwegciwane nucleic acid |
| I-ROX | umkhuhlane A H3 uhlobo lwegciwane nucleic acid |
| CY5 | ukulawula kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | i-nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Amakhophi/μL |
| Ukucaciswa | Akukho ukusabela okuphambene namanye amasampula okuphefumula afana nomkhuhlane A, Umkhuhlane B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneuvirus A1/A2 B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, njll. kanye ne-human genomic DNA. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems I-SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
I-Nucleic Acid Extraction noma I-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd.Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalelo yokusetshenziswa.Ivolumu yesampula ekhishiwe ingu-140μL, futhi ivolumu ye-lution enconyiwe ingu-60μL.
Inketho yesi-2.
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalelo yokusetshenziswa.Ivolumu yesampula ekhishiwe ingu-200μL, futhi ivolumu ye-lution enconyiwe ingu-80μL.
