Umkhuhlane virus Universal / H1 / H3
Igama lomkhiqizo
I-HWTS-RT012 Umkhuhlane I-Virus Universal / H1 / H3 Nucleic Acid Multiplex Detection Detection Detection)
I-Epidemiology
Igciwane lomkhuhlane liyizinhlobo ezimele ze-Orthomyxoviridae. Yi-pathogen esisongela kakhulu impilo yabantu. Kungatheleleka kakhulu umgcini. Ubhubhane lwesizini luthinta abantu ababalelwa ezigidini ezingama-600 emhlabeni wonke futhi kubangela ukufa kwabantu abangama-250,000 ~ 500,000, lapho igciwane liyimbatho eyimbangela yokutheleleka nokufa. Umkhuhlane Igciwane liyi-RNA engemihle engashadi. Ngokusho kwayo i-hemagglutinin (HA) ne-Neuraminidase Phakathi komkhuhlane amagciwane, ama-subtype ama-virus anomkhuhlane angatheleleka ngqo kubantu yile: I-H1N1, H3N2, H5N1, H7N3, H7N2, H7N3, H3N2 neH10N8 ne-H10N8. Phakathi kwazo, i-H1 kanye ne-H3 ama-subtypes ane-pathogenic kakhulu, futhi kufanelekile ukunakwa.
Insungubezi
| -Yenza | umkhuhlane we-virus hlobo virus nucleic acid |
| VIC / Hex | Umkhuhlane A H1 Type Virus nucleic acid |
| Ivupho | Umkhuhlane A H3 Type Virus nucleic acid |
| Cy5 | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | nasopharyngel swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | Amakhophi angama-500 / μL |
| Ukucacisa | AKUKHO BANYE UKUXHUMANISA KWAMAHHALA namanye amasampula okuphefumula anjenge-A, Umkhuhlane B, Legisonalla q Fever, Adenovirus, 3, 3, i-Perapneumovirus A1 / A2 / B1 / B2, I-virus ebonakalayo yokuvumelanisa A / B, iCoronavirus 229E / NL63 / Hku1 / OC43, Rhinovirus A / B / C, i-Chlamydia Trachomatis, i-Adenovirus, njll. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Plus real-time PCCRECT SYPELY (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Ukukhishwa kwe-nucleic acid noma i-reagent yokuhlanza (ypp15-R) nguTiangen Biotech (Beijing) Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalo yokusebenzisa. IVolumu yesampula ekhishwe i-140μL, kanti ivolumu yokuqedwa enconyiwe ingu-60μL.
Inketho 2.
I-Macro & Micro-Tevation Viral DNA / RNA KIT (HWTS-3004-32, HWTS-3004-48, HWS-3004-96) ne-MACRO-3004-9 (HWS-3006C, HWS-3006C). Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalo yokusebenzisa. Ivolumu yesampula ekhishwe i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-80μL.
