Umkhuhlane A Virus Universal/H1/H3
Igama lomkhiqizo
I-HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
I-Epidemiology
Igciwane lomkhuhlane liwuhlobo olumele i-Orthomyxoviridae. Kuyi-pathogen esongela kakhulu impilo yomuntu. Ingathelela umsingathi kakhulu. Ubhubhane lwesizini luthinta abantu ababalelwa ezigidini ezingama-600 emhlabeni wonke futhi lubangela ukufa kwabantu abangu-250,000 ~ 500,000, okukhona igciwane lomkhuhlane A eliyimbangela enkulu yokutheleleka nokufa. Igciwane loMkhuhlane A liyi-RNA enomucu owodwa onegayo. Ngokusho kwe-surface hemagglutinin (HA) kanye ne-neuraminidase (NA), i-HA ingahlukaniswa ngama-subtypes ayi-16, i-NA Ihlukaniswe yaba yi-9 subtypes. Phakathi kwamagciwane omkhuhlane A, izinhlobo ezincane zamagciwane omkhuhlane ezingathelela abantu ngokuqondile yilezi: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 kanye ne-H10N8. Phakathi kwazo, i-H1 ne-H3 subtypes i-pathogenic kakhulu, futhi ifaneleka ngokukhethekile ukunakwa.
Isiteshi
| FAM | influenza Uhlobo lwe-universal virus nucleic acid |
| VIC/HEX | umkhuhlane A H1 uhlobo lwegciwane nucleic acid |
| I-ROX | umkhuhlane A H3 uhlobo lwegciwane nucleic acid |
| CY5 | ukulawula kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | i-nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Amakhophi/μL |
| Ukucaciswa | Akukho ukuphinda kusebenze namanye amasampula okuphefumula afana nomkhuhlane A, Umkhuhlane B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapnevirus A1/A1/A2u syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, njll. kanye ne-DNA yegenomic yomuntu. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems I-SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 ye-Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
I-Nucleic Acid Extraction noma I-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalelo yokusetshenziswa. Ivolumu yesampula ekhishiwe ingu-140μL, futhi ivolumu ye-lution enconyiwe ingu-60μL.
Inketho yesi-2.
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalelo yokusetshenziswa. Ivolumu yesampula ekhishiwe ingu-200μL, futhi ivolumu ye-lution enconyiwe ingu-80μL.
