Igciwane le-Influenza A eliyi-Universal/H1/H3

Isithombe Esifakiwe se-Influenza A Virus Universal/H1/H3

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola igciwane le-influenza A eliyi-universal type, uhlobo lwe-H1 kanye ne-H3 nucleic acid kumasampula e-nasopharyngeal swab yomuntu.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola I-HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex (i-Fluorescence PCR)

I-Epidemiology

Igciwane lomkhuhlane liwuhlobo olumele i-Orthomyxoviridae. Liyigciwane elisongela impilo yabantu kakhulu. Lingathelela umninimzi kakhulu. Ubhubhane lwezinkathi luthinta abantu ababalelwa ezigidini ezingu-600 emhlabeni wonke futhi lubangela ukufa kwabantu abangu-250,000 ~ 500,000, lapho igciwane lomkhuhlane A liyimbangela eyinhloko yokutheleleka nokufa. Igciwane lomkhuhlane A liyi-RNA enezintambo ezinde ezinezintambo ezinde. Ngokusho kwe-hemagglutinin yayo engaphezulu (HA) kanye ne-neuraminidase (NA), i-HA ingahlukaniswa ngezinhlobo ezingu-16, i-NA ihlukaniswe ngezinhlobo ezingu-9. Phakathi kwamagciwane omkhuhlane A, izinhlobo zamagciwane omkhuhlane angathelela abantu ngqo yilezi: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 kanye ne-H10N8. Phakathi kwazo, izinhlobo ze-H1 kanye ne-H3 zibangela izifo kakhulu, futhi zifanele ukunakwa ngokukhethekile.

Isiteshi

I-FAM	i-influenza A igciwane eliwuhlobo lwe-universal type nucleic acid
I-VIC/HEX	igciwane le-nucleic acid lohlobo lwe-influenza A H1
I-ROX	igciwane le-H3 uhlobo lwe-influenza A nucleic acid
CY5	ukulawula kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	≤-18℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-9
Uhlobo Lwesifanekiso	i-nasopharyngeal swab
Ct	≤38
CV	≤5.0%
I-LoD	Amakhophi angu-500/μL
Ukucaciswa	Akukho ukuhlangana kwezifo nezinye izibonelo zokuphefumula ezifana ne-Influenza A, i-Influenza B, i-Legionella pneumophila, i-Rickettsia Q fever, i-Chlamydia pneumoniae, i-Adenovirus, i-Respiratory Syncytial Virus, i-Parainfluenza 1, 2, 3, i-Coxsackie virus, i-Echo virus, i-Metapneumovirus A1/A2/B1/B2, i-Respiratory syncytial virus A/B, i-Coronavirus 229E/NL63/HKU1/OC43, i-Rhinovirus A/B/C, i-Boca virus 1/2/3/4, i-Chlamydia trachomatis, i-adenovirus, njll. kanye ne-human genomic DNA.
Izinsimbi Ezisebenzayo	Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR

Ukugeleza Komsebenzi

I-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokwemiyalelo yokusebenzisa. Umthamo wesampula okhishwe ngu-140μL, kanti umthamo wokuhlanza onconywayo ngu-60μL.

Inketho 2.

I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhipha kufanele kwenziwe ngokuqinile ngokwemiyalelo yokusebenzisa. Umthamo wesampula okhishwe ungama-200μL, kanti umthamo wokususa onconywayo ungama-80μL.

Okwedlule: Igciwane le-Ebola laseZaire

Olandelayo: I-Gene Yokumelana Ne-Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)

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