I-Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Igama lomkhiqizo
I-HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Ikhithi Yokubona (i-Fluorescence PCR)
I-Epidemiology
Ama-antibiotic e-Carbapenem angama-atypical β-lactam antibiotics ane-spectrum ye-antibacterial ebanzi kakhulu nomsebenzi onamandla wokulwa namagciwane.Ngenxa yokuqina kwayo ku-β-lactamase kanye nobuthi obuphansi, isibe ngomunye wemithi elwa namagciwane ebaluleke kakhulu ekwelapheni izifo ezinzima zamagciwane.I-Carbapenems izinzile kakhulu kuma-β-lactamase (ESBLs), ama-chromosomes, nama-plasmid-mediated cephalosporinases (ama-AmpC enzymes).
Isiteshi
| I-PCR-Mix 1 | I-PCR-Mix 2 | |
| FAM | I-IMP | I-VIM |
| VIC/HEX | Ukulawula Kwangaphakathi | Ukulawula Kwangaphakathi |
| CY5 | NDM | I-KPC |
| I-ROX | OXA48
| OXA23 |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isikhwehlela, amakoloni ahlanzekile, i-rectal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103I-CFU/mL |
| Ukucaciswa | a) Ikhithi ithola izinkomba ezingezinhle zenkampani ezisezingeni, futhi imiphumela ihlangabezana nezidingo zezinkomba ezihambisanayo. b) Imiphumela yokuhlolwa kwe-cross-reactivity ibonisa ukuthi le kit ayinakho ukusabela okuphambene namanye amagciwane okuphefumula, afana ne-Klebsiella pneumoniae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa, i-Streptococcus pneumoniae, i-Neisseria meningitidis, i-Staphylococcus aureus, i-Klexytobacine e-Aureus, i-Klexytobacinella, i-Klexytobacinela junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, noma amasampula aqukethe ezinye izinhlobo zofuzo ezimelana nezidakamizwa i-CTX, mecVA, TEM, njll. c) Ukunqanda ukuphazamiseka: I-Mucin, i-Minocycline, i-Gentamicin, i-Clindamycin, i-Imipenem, i-Cefoperazone, i-Meropenem, i-Ciprofloxacin Hydrochloride, i-Levofloxacin, i-Clavulanic acid, i-Roxithromycin ikhethelwa ukuhlolwa kokuphazanyiswa, futhi imiphumela ibonisa ukuthi izinto eziphazamisayo ezibalwe ngenhla azinakho ukusabela okuphazamisayo. ekutholweni kwezakhi zofuzo zokumelana ne-carbapenem i-KPC, i-NDM, i-OXA48, i-OXA23, i-VIM, ne-IMP. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System (I-FQD-96A,I-HangzhouUbuchwepheshe be-Bioer) I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nge-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Engeza u-200μL kasawoti ojwayelekile ku- imvula ye-thallus.Izinyathelo ezilandelayo kufanele zilandele imiyalelo yokukhipha, kanye nevolumu ye-lution enconyiwe100μL.
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co., Ltd. Ukukhishwa kufanele kuqalwe ngokuhambisana ngokuqinile nesinyathelo sesi-2 somyalelo ozosetshenziswa (engeza u-200μL we-buffer GA kumvula yethallus. , futhi unyakazise kuze kube yilapho i-thallus imisiwe ngokuphelele).Sebenzisa amanzi amahhala e-RNase/DNase ukuze ukhanyise, futhi ivolumu ye-elution enconyiwe ingu-100μL.
Inketho yesi-3.
Isisebenzi sokukhipha esinconyiwe: I-Macro & Micro-Test Sample Release Reagent.Isampula lesikhwehlela sidinga ukugezwa ngokungeza u-1mL wosawoti ojwayelekile kumvula yethallus ephathwayo eshiwo ngenhla, ifakwe phakathi ku-13000r/min imizuzu emi-5, futhi i-supernatant iyalahlwa (gcina u-10-20µL we-supernatant).Ukuze uthole ikholoni emsulwa kanye ne-rectal swab, engeza u-50μL we-reagent yokukhishwa kwesampula ngokuqondile kumvula ye-thallus ephathwayo eshiwo ngenhla, futhi izinyathelo ezilandelayo kufanele zikhishwe ngokuya ngomyalelo wokusetshenziswa.
