I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii kanye ne-Pseudomonas Aeruginosa kanye ne-Drug Resistance Genes (i-KPC, i-NDM, i-OXA48 kanye ne-IMP) Multiplex
Igama lomkhiqizo
I-HWTS-RT109 Klebsiella Pneumoniae, i-Acinetobacter Baumannii kanye ne-Pseudomonas Aeruginosa kanye ne-Drug Resistance Genes (i-KPC, i-NDM, i-OXA48 kanye ne-IMP) Multiplex Detection Kit (i-Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Klebsiella pneumoniae iyi-pathogen evamile yezokwelapha ethola amathuba futhi ingenye yamagciwane abalulekile abangela izifo ezibangelwa yi-nosocomial. Lapho ukumelana komzimba kuncipha, amagciwane angena emaphashini esuka emgudwini wokuphefumula, abangele ukutheleleka ezingxenyeni eziningi zomzimba, futhi ukusetshenziswa kwangaphambi kwesikhathi kwama-antibiotic kuyisihluthulelo sokwelapha.[1].
Indawo evame kakhulu yokutheleleka yi-Acinetobacter baumannii amaphaphu, okuyisifo esibalulekile se-Hospital acquired pneumonia (HAP), ikakhulukazi i-Ventilator associated pneumonia (VAP). Ivame ukuhambisana nezinye izifo zamagciwane kanye nesikhunta, ezinezici zezinga eliphezulu lokugula kanye nezinga eliphezulu lokufa.
I-Pseudomonas aeruginosa iyi-bacilli engeyona i-gram-negative evame kakhulu emtholampilo, futhi iyi-pathogen ebalulekile etholakala esibhedlela, enezici zokungena kalula emakhaya, ukuhlukahluka okulula kanye nokumelana nemithi eminingi.
Isiteshi
| Igama | I-PCR-Mix 1 | I-PCR-Mix 2 |
| Isiteshi se-FAM | Aba | I-IMP |
| Isiteshi se-VIC/HEX | Ukulawula Kwangaphakathi | I-KPC |
| Isiteshi se-CY5 | PA | I-NDM |
| Isiteshi se-ROX | I-KPN | I-OXA48 |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isikhwehlela |
| Ct | ≤36 |
| CV | ≤10.0% |
| I-LoD | 1000 CFU/mL |
| Ukucaciswa | a) Ukuhlolwa kokusabela okuxubile kukhombisa ukuthi le khithi ayinayo i-reactivity exubile nezinye izifo zokuphefumula, njenge-Streptococcus pneumoniae, i-Neisseria meningitidis, i-Staphylococcus aureus, i-Klebsiella oxytoca, i-Haemophilus influenzae, i-Acinetobacter jelly, i-Acinetobacter hemolytica, i-Legionella pneumophila, i-Escherichia coli, i-Pseudomonas fluorescens, i-Candida albicans, i-Chlamydia pneumoniae, i-Respiratory Adenovirus, amasampula e-Enterococcus kanye ne-sputum angenazo izinjongo, njll. b) Amandla okulwa nokuphazamiseka: Khetha i-mucin, i-minocycline, i-gentamicin, i-clindamycin, i-imipenem, i-cefoperazone, i-meropenem, i-ciprofloxacin hydrochloride, i-levofloxacin, i-clavulanic acid, kanye ne-roxithromycin, njll. ukuze kuhlolwe ukuphazamiseka, futhi imiphumela ikhombisa ukuthi izinto zokuphazamiseka okukhulunywe ngazo ngenhla aziphazamisi ukutholakala kwe-Klebsiella pneumoniae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa kanye ne-carbapenem resistance genes KPC, NDM, OXA48 kanye ne-IMP. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Isixazululo se-PCR Esiphelele
