I-Klebsiella pneumonia, i-acinetbacter bauumannii kanye ne-pseudomonas aeruginosa kanye nokhetho lokumelana nezidakamizwa (KPC, NDM, OXA48 ne-Imp) Multex
Igama lomkhiqizo
I-HWSTS-RT109 Klebsiella pneumonia, i-acinetbacras aeruginosa kanye nofuzo lokumelana nezidakamizwa (KPC, NDM, OXA48 ne-Imp)
Isitifiketi
CE
I-Epidemiology
I-Klebsiella Pneumoniae iyi-pathogen evamile yamathuba emitholampilo futhi elinye lamagciwane abalulekile e-pathogenic abangela ukutheleleka kwe-nosocomial. Lapho ukumelana komzimba kuncishiswa, amagciwane angena emaphashini avela epheshana lokuphefumula, abangela ukutheleleka ezingxenyeni eziningi zomzimba, futhi ukusetshenziswa kwangaphambili kwama-antibiotics kuyisihluthulelo sokwelashwa[1].
Isayithi elivame kakhulu le-AcinetOBanctii Efnuengiles lingamaphaphu, okuyi-pathogen ebalulekile ye-Pneumonia etholakele esibhedlela (HAp), ikakhulukazi i-ventilator ehambisana ne-pneumonia (i-VAP). Imvamisa ihambisana nezinye izifo ezingama-bacterial and fungal, ngezimpawu zezinga eliphakeme lokufa kwabantu kanye nezinga eliphakeme lokufa kwabantu.
I-Pseudomonas Aeruginosa iyi-bacilli evame kakhulu engavuli i-gram-hermetive e-bacilli emitholampilo, futhi i-pathogen ebalulekile yamathuba okutheleleka esibhedlela, nezimpawu zokuvumelanisa okulula, ukuhlukahluka kwezidakamizwa ezahlukahlukene.
Insungubezi
Ibizo | Pcr-mix 1 | Pcr-mix 2 |
Isiteshi se-Fam | Tha | Ind |
Isiteshi seVic / Hex | Ukulawulwa Kwangaphakathi | I-KPC |
Isiteshi seCy5 | PA | Ndm |
Isiteshi se-ROX | Kpn | I-OXA48 |
Amapharamitha Ezobuchwepheshe
Ukubeka | ≤-18 ℃ |
Ishalofu-impilo | Izinyanga eziyi-12 |
Uhlobo lwe-Specimen | Sputum |
Ct | ≤36 |
CV | ≤10.0% |
Indlu edayisa insimbi | I-1000 CFU / ML |
Ukucacisa | a) Ukuhlolwa kokuphambanisa nokusebenza kabusha kukhombisa ukuthi le khithi ayinakho ukubhekisisa ama-pathogen ahlanganayo, njenge-streptococcus areumoniae, i-staphylococcus aureus, i-haemophilus jelly, i-acinet hemolytica, i-acionella pneumophila, e-linetobacta pneumophica, I-Escherhichia Coli, i-Pseudomonas Floorescens, i-Candicas Alicans, i-Chlamydia pneumoniae, i-adenovirus yokuphefumula, amasampula e-sputum ngaphandle kwamatshe, njll. b) Ikhono lokulwa nokuphanga Ungaphazamisi ukutholwa E-Klebsiella Pneumonia, i-acinetbactas baumannii, i-pseudomonas aeruginosa ne-carbapenem ukumelana nohlobo lwe-genes KPC, NDM, OXA48 kanye ne-Imp. |
Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 kanye nohlelo lwe-Real-Time Pcc Retection System (FQD-96A, ubuchwepheshe be-bioer) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |