I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii ne-Pseudomonas Aeruginosa kanye Nezakhi Zofuzo Ezimelana Nezidakamizwa (KPC, NDM, OXA48 kanye ne-IMP) Multiplex

I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii ne-Pseudomonas Aeruginosa kanye Nezakhi Zofuzo Zokumelana Nezidakamizwa (KPC, NDM, OXA48 kanye ne-IMP) Isithombe Esifakiwe Esiningi

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwekhwalithi ye-in vitro ye-Klebsiella pneumoniae (KPN), i-Acinetobacter baumannii (Aba), i-Pseudomonas aeruginosa (PA) kanye nezakhi zofuzo ezine ze-carbapenem (okuhlanganisa i-KPC, i-NDM, i-OXA48 ne-IMP) kumasampula esikhwehlela somuntu, ukuhlinzeka. isisekelo sesiqondiso sokuxilongwa komtholampilo, ukwelashwa kanye nemithi yeziguli ezine-infection ye-bacterial okusolakala ukuthi.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-RT109 Klebsiella Pneumoniae, i-Acinetobacter Baumannii ne-Pseudomonas Aeruginosa kanye Nezakhi Zofuzo Ezimelana Nezidakamizwa (KPC, NDM, OXA48 kanye ne-IMP) Ikhithi Yokuthola I-Multiplex(Fluorescence PCR)

Isitifiketi

I-Epidemiology

I-Klebsiella pneumoniae iyisifo esivamile somtholampilo esingosomathuba futhi ingelinye lamabhaktheriya abalulekile e-pathogenic abangela izifo ze-nosocomial.Lapho ukumelana komzimba kuncipha, amagciwane angena emaphashini ephuma emgudwini wokuphefumula, abangele ukutheleleka ezingxenyeni eziningi zomzimba, futhi ukusetshenziswa kokuqala kwama-antibiotic kuyisihluthulelo sokwelapha.^[1].

Indawo evamile yokutheleleka nge-Acinetobacter baumannii amaphaphu, okuyigciwane elibalulekile le-Hospital gained pneumonia (HAP), ikakhulukazi i-Ventilator associated pneumonia (VAP).Ivamise ukuhambisana nezinye izifo ezibangelwa amagciwane kanye nesikhunta, ezinezici zezinga eliphezulu lokugula kanye nezinga eliphezulu lokufa.

I-Pseudomonas aeruginosa iyigciwane le-non-fermentative gram-negative elivame kakhulu emisebenzini yokwelapha, futhi iyigciwane elibalulekile elivula ithuba lokutheleleka okutholwa esibhedlela, elinezici zokuba lula kwekoloni, ukuhluka okulula kanye nokumelana nezidakamizwa eziningi.

Isiteshi

Igama	I-PCR-Mix 1	I-PCR-Mix 2
Isiteshi se-FAM	Aba	I-IMP
Isiteshi se-VIC/HEX	Ukulawula Kwangaphakathi	I-KPC
Isiteshi se-CY5	PA	NDM
Isiteshi se-ROX	I-KPN	OXA48

Imingcele Yezobuchwepheshe

Isitoreji	≤-18℃
Impilo yeshelufu	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	Isikhwehlela
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/mL
Ukucaciswa	a) Ukuhlolwa kwe-cross-reactivity kubonisa ukuthi le khithi ayinakho ukuphinda isebenze kabusha namanye amagciwane okuphefumula, afana ne-Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter, Le-Pleefilas, Le-Pleefilas, Le-Plebsiella oxytoca. fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus kanye namasampula isikhwehlela ngaphandle target, njll. b)Amandla okunqanda ukuphazamiseka: Khetha i-mucin, i-minocycline, i-gentamicin, i-clindamycin, imipenem, i-cefoperazone, i-meropenem, i-ciprofloxacin hydrochloride, i-levofloxacin, i-clavulanic acid, ne-roxithromycin, njll. ngokuhlolwa kokuphazanyiswa, futhi imiphumela ibonisa ukuthi izinto eziphazamisayo ezishiwo ngenhla ungaphazamisi ukutholwa kwe-Klebsiella pneumoniae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa kanye nezakhi zofuzo zokumelana ne-carbapenem i-KPC, i-NDM, i-OXA48 ne-IMP.
Izinsimbi Ezisebenzayo	I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio^®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-LightCycler^®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Bioer) I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System