Igciwane le-Measles Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola Igciwane Le-Measles Virus Nucleic Acid ye-HWTS-RT028 (i-Fluorescence PCR)
I-Epidemiology
I-Measles yisifo esithathelwanayo sokuphefumula esibangelwa yigciwane le-measles. Ngokwezokwelapha sibonakala ngomkhuhlane, ukuvuvukala kwendlela yokuphefumula ephezulu, i-conjunctivitis, ama-erythematous papules esikhumbeni, kanye namabala e-kopik ku-buccal mucosa. Iziguli ezine-measles ziwukuphela komthombo wokutheleleka kwe-measles, edluliselwa kakhulu ngamaconsi okuphefumula, futhi isixuku ngokuvamile sisengozini. Igciwane le-measles liyathelelana kakhulu futhi lisakazeka ngokushesha, okungadala ukuqubuka kalula futhi lingenye yezifo ezithelelanayo ezibeka impilo nempilo yezingane engozini enkulu.
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | uketshezi lwe-herpes, ama-oropharyngeal swabs |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Amakhophi angu-500/μL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR.
Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-EudemonTM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.
