I-Mumps Virus Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-RT029-Mumps Virus Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Igciwane le-mumps liyigciwane elilodwa le-serotype, kodwa i-SH protein gene iyahlukahluka kakhulu kuma-mump virus ahlukene. Igciwane le-mumps lihlukaniswe ngama-genotypes angu-12 ngokusekelwe ekwehlukeni kwama-SH protein genes, okungukuthi izinhlobo A, B, C, D, F, G, H, I, J, K, L, kanye no-N. Ukusatshalaliswa kwama-genotypes ama-mump virus kunezici ezisobala zesifunda. Izinhlobo ezivame eYurophu ikakhulukazi ama-genotypes A, C, D, G, kanye no-H; izinhlobo ezivame kakhulu eMelika ama-genotypes C, D, G, H, J, kanye no-K; izinhlobo ezivame kakhulu e-Asia ama-genotypes B, F, I, kanye no-L; uhlobo oluvame kakhulu eShayina i-genotype F; izinhlobo ezivame kakhulu eJapan naseNingizimu Korea ama-genotypes B kanye no-I ngokulandelana. Akucaci ukuthi lolu hlobo lwegciwane olusekelwe ku-SH gene lubalulekile yini ocwaningweni lokugoma. Njengamanje, izinhlobo zokugoma ezisetshenziswa emhlabeni wonke ikakhulukazi ziyi-genotype A, kanti ama-antibodies akhiqizwa ama-antigens ama-virus ezinhlobo ezahlukene ze-genotypes avikela ngokuvundlile.
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi ayi-1000/mL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-EudemonTM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.
