● Isifo Sofuba Se-Mycobacterium
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I-Mycobacterium Tuberculosis Nucleic Acid kanye ne-Rifampicin(RIF)，Resistance(INH)
Le khithi isetshenziselwa ukuthola i-Mycobacterium tuberculosis DNA ngaphakathi kwesibeletho, isiko eliqinile (i-LJ Medium) kanye nesiko loketshezi (i-MGIT Medium), uketshezi lokuhlanza i-bronchial, kanye nokuguqulwa kwezakhi zofuzo esifundeni se-codon esingu-507-533 amino acid (isifunda esingu-81bp, esinquma ukumelana ne-rifampicin) sejini le-rpoB lokumelana ne-Mycobacterium tuberculosis rifampicin, kanye nokuguqulwa kwezakhi zofuzo ezindaweni eziyinhloko zokuguqulwa kwezakhi zofuzo zokumelana ne-isoniazid ye-Mycobacterium tuberculosis. Inikeza usizo ekuxilongweni kokutheleleka kwe-Mycobacterium tuberculosis, futhi ithola izakhi zofuzo eziyinhloko zokumelana ne-rifampicin ne-isoniazid, okusiza ukuqonda ukumelana nemithi kwe-Mycobacterium tuberculosis etheleleke yisiguli.
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Ukuguqulwa kwe-Mycobacterium Tuberculosis INH
Leli khithi lifanele ukutholakala kwezindawo eziyinhloko zokuguqulwa kwezakhi zofuzo kumasampula esikhwehlela somuntu aqoqwe ezigulini ezine-Tubercle bacillus eziholela ku-mycobacterium tuberculosis INH: Isifunda se-InhA promoter -15C>T, -8T>A, -8T>C; Isifunda se-AhpC promoter -12C>T, -6G>A; i-homozygous mutation ye-KatG 315 codon 315G>A, 315G>C.
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I-Mycobacterium Tuberculosis Ukumelana ne-Rifampicin
Le khithi ifaneleka ekutholakaleni kwekhwalithi yokuguqulwa kwe-homozygous esifundeni se-amino acid esingu-507-533 se-amino acid se-rpoB gene esibangela ukumelana ne-Mycobacterium tuberculosis rifampicin.
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Ukumelana ne-Mycobacterium Tuberculosis Nucleic Acid kanye ne-Rifampicin
Le khithi ifanelekela ukutholakala kwe-Mycobacterium tuberculosis DNA kumasampula esikhwehlela somuntu ngaphakathi kwe-vitro, kanye nokuguqulwa kwe-homozygous esifundeni se-codon se-amino acid esingu-507-533 se-gene ye-rpoB ebangela ukumelana ne-Mycobacterium tuberculosis rifampicin.
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I-DNA ye-Mycobacterium Tuberculosis
Ifanele ukutholakala kwe-DNA yesifo sofuba se-Mycobacterium kumasampula esikhwehlela somtholampilo womuntu, futhi ifanele ukuhlonza okusizayo ukutheleleka kwesifo sofuba se-Mycobacterium.
