I-Mycoplasma hominis, i-Ureaplasma urealyticum ne-Gardnerella vaginalis Nucleic Acid
Igama lomkhiqizo
I-HWTS-UR044-Mycoplasma hominis, i-Ureaplasma urealyticum kanye ne-Gardnerella vaginalis Nucleic Acid Detection Kit (i-Fluorescence PCR)
I-Epidemiology
I-Mycoplasma hominis (MH) uhlobo lwe-mycoplasma olukhona emgudwini womchamo kanye nezitho zangasese futhi lungabangela ukutheleleka komgudu womchamo kanye nokuvuvukala kwezitho zangasese. I-Mycoplasma hominis ikhona kakhulu emvelweni futhi ihlotshaniswa nezinhlobo ezahlukene zokutheleleka komgudu womchamo njenge-nongonococcal urethritis, i-cervicitis yabesifazane, i-adnexitis, ukungazali, njll. I-Ureaplasma urealyticum (UU) iyi-microorganism encane kakhulu ye-prokaryotic celled ephakathi kwamagciwane namagciwane futhi ingaphila ngokuzimela, futhi futhi iyi-microorganism ebangela kalula ukutheleleka komgudu womchamo kanye nomgudu womchamo. Kwabesilisa, ingabangela i-prostatitis, i-urethritis, i-pyelonephritis, njll.; kwabesifazane, ingabangela ukusabela kokuvuvukala emgudwini womchamo njenge-vaginitis, i-cervicitis, kanye nesifo sokuvuvukala kwe-pelvic, futhi ingenye yezifo ezibangela ukungazali kanye nokukhulelwa kwesisu. Imbangela evame kakhulu ye-vaginitis kwabesifazane yi-bacterial vaginosis, kanti i-bacterial pathogenic ebalulekile ye-bacterial vaginosis yi-Gardnerella vaginalis. I-Gardnerella vaginalis (GV) iyigciwane elithola amathuba elingabangeli izifo uma likhona ngamanani amancane. Kodwa-ke, lapho amagciwane amakhulu esithweni sangasese sowesifazane i-Lactobacilli encishisiwe noma eqedwa, okubangela ukungalingani endaweni yesitho sangasese sowesifazane, i-Gardnerella vaginalis iyanda ngamanani amakhulu, okuholela ku-bacterial vaginosis. Ngesikhathi esifanayo, amanye amagciwane (njenge-Candida, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, njll.) angase ahlasele umzimba womuntu, okubangela i-mixed vaginitis kanye ne-cervicitis. Uma i-vaginitis kanye ne-cervicitis zingatholakali futhi zingalashwa ngendlela efanele futhi ngesikhathi esifanele, kungase kube nokutheleleka okukhuphukayo yizifo ezithinta i-mucosa yomgudu wokuzala, okuholela kalula ekuthelelekeni komgudu wokuzala ophezulu njenge-endometritis, i-salpingitis, i-tubo-ovarian abscess (TOA), kanye ne-pelvic peritonitis, okungaholela ezinkingeni ezinkulu njengokungazali, ukukhulelwa kwe-ectopic ngisho nemiphumela emibi yokukhulelwa.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-swab yesilisa ye-urethra, i-swab yesibeletho yesifazane, i-swab yesitho sangasese sowesifazane |
| Ct | ≤38 |
| CV | ＜5.0% |
| I-LoD | I-UU, GV 400 Amakhophi/mL; MH 1000 Amakhophi/mL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-EudemonTM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.
