Amagciwane okuphefumula ahlanganisiwe
Igama lomkhiqizo
I-HWTS-RT155A PATOGEENT PATOGEENCE INDLELA YOKUTHOLAKALA KOKUTHOLAKALA (I-Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
Isifo segciwane leCorona 2019, okukhulunywa ngaye'I-COVID 19', kubhekiswa ku-pneumonia okubangelwa ukutheleleka ngo-2019-NCOV. I-2019-NCOV yi-coronavirus eyi-β runes. I-COVIIG-19 yisifo esithelelekayo sokuphefumula esinamandla, futhi inani labantu livame ukwenzeka. Njengamanje, umthombo wokutheleleka uneziguli ikakhulukazi ezitheleleke ngo-2019-NCOV, futhi abantu abanegciwane le-asymptomatic batheleleke nabo bangaba ngumthombo wokutheleleka. Ngokusekelwe ophenyweni lwezempi lwamanje, isikhathi sokufakwa kwezinsuku eziyi-1-14, iningi lezinsuku ezingama-3-7. Umkhuhlane, ukukhwehlela owomile nokukhathala kungukubonakaliswa okuyinhloko. Iziguli ezimbalwa zazinezimpawu ezifana nokuxinana kwamakhala, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nohudo, njll.
Umkhuhlane, ojwayele owaziwa ngokuthi "umkhuhlane", yisifo esibuhlungu sokuphefumula esidalwa yigciwane lomkhuhlane. Kuyatheleleka kakhulu. Idluliselwa ikakhulukazi ngokukhwehlela nokushushuluza. Imvamisa ihlukana entwasahlobo nasebusika. Amagciwane omkhuhlane ahlukaniswe yaba ngumkhuhlane a -I-RNA Virus ehlukanisiwe. Umkhuhlane Igciwane lingukutheleleka okuphezulu kokuphefumula, kufaka phakathi i-H1N1, H3N2 namanye ama-subtypes, athambekele ekuguqukeni nasekuqubukeni emhlabeni wonke. I- "Shift" ibhekisela ekuguqukeni komkhuhlane ngegciwane, okuholele ekuqubukeni kwegciwane elisha elithi "subtype". Umkhuhlane B Amagciwane ahlukaniswe ngamabala amabili, iYamagata neVictoria. Umkhuhlane B Virus kuphela une-antiigenic drift, futhi kuthola ukubhekwa kwamasosha omzimba womuntu nokuqedwa ngokuguqulwa kwayo. Kodwa-ke, ijubane lokuziphendukela kwemvelo le-LogeENENeza B Virus lihamba kancane kunaleso somkhuhlane womuntu igciwane. Igciwane le-Informen B lingabuye libangele izifo zokuphefumula komuntu futhi ziholele ezinhlanzini.
Igciwane le-respiratory syncytial virus (RSV) yigciwane le-RNA, elingelamndeni wePharamyxoviridae. Kudluliselwa ngamaconsi amoya kanye nokuxhumana okusondelene futhi yi-pathogen eyinhloko yokutheleleka okuphansi kokuphefumula kwezingane. Izinsana ezitheleleke nge-RSV zingahlakulela i-bronchiolitis enzima ne-pneumonia, ezihlobene nesifuba sezinto zomoya. Izinsana zinezimpawu ezinzima, kufaka phakathi umkhuhlane ophakeme, i-rhinitis, i-pharyngitis kanye ne-laryngitis, bese kuthi i-bronchiolitis ne-pneumonitia. Izingane ezimbalwa ezigula zingaba yinkimbinkimbi ngemidiya ye-Otitis, i-Pleurisy naseMyOCarditis, njll. Upper Upper Respiratory Tract Tract uwuphawu oluphambili lokutheleleka kubantu abadala nasezinganeni ezindala.
Insungubezi
| -Yenza | I-SARS-CoV-2 |
| I-VIC (Hex) | I-RSV |
| Cy5 | IFV a |
| Ivupho | IFV B |
| Quasar 705 | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | -18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | I-Oropharyngengeal Swapy |
| Ct | ≤38 |
| Indlu edayisa insimbi | Ngo-2019-NCOV: 300Copies / ML Umkhuhlane virus / umkhuhlane b virus / ukuphefumula kwe-syncytial virus: 500copies / ml |
| Ukucacisa | a) Imiphumela yokusebenzisa kabusha i-Cross-Recivity ikhombisa ukuthi akukho ukusabela kwesiphambano phakathi kwekhithi ne-coronavirus sarsr-cov, i-HCOV-OCU1, HCOV-NL63, HCOV-NL63, 2, 3, Rhinovirus A, B, C, Chlamydia Pneumoniae, Metapneumovirus lomuntu, I-Enterovirus A, B, C, D, i-Epsterin-Barl Virus, i-virus yesimumbile, i-norovetella ye-noronirus, i-abuemophilus agerus, i-staphylococcus aureus, i-steptococcus pneumoniae, I-Streptococcus Pyogene, Klebsactella Pneumoniae, i-Mycobactelium Tuberculosis, Smoke Aspergillus, Candica Abicrans, Pneumocystis Jiroveci kanye Nentsha I-Cryptococcus ne-Human genomic nucleic acid. b) Ikhono lokulwa nokuphanga , eclomethasone (20mg / ml), Dexamethasone (20mg / mL), I-Flunesisolide (20Cinolone Acetonide (2mg / ML), i-Budesonide (2mg / ML), i-MomeTasone (2mg / ml), i-fluticasone (2mg / ml), i-arpha interferoon (800IU / ML ), zanamivir (20mg / ml), i-ribavirin (10mg / ml), i-oseltamivir (60ng / ml), i-peramivir (1mg / ml), i-ritonavir (60mg / ml), i-mupirocin (20mg / ml), i-azithromycin ( I-1mg / ml), i-CEFTRIAXOne (40Cing / ML), iMeropenem (200mg / ML), i-levefloxacin (10μg / ml) kanye ne-tobramycin (0.6mg / ML) ukuze kuhlolwe amandla okuphazamisa, futhi imiphumela ibonisa ukuthi ukuguquguquka okushiwo ngenhla kwemiphumela yokuhlolwa ama-pathogen. |
| Izinsimbi ezisebenzayo | I-Biorad CFX96 Uhlelo lwe-Real-Time PCR I-Rotor-Gene Q 5plex HRM Ipulatifomu ye-Real-Time PCRY System |
