Amagciwane Okuphefumula Ahlanganisiwe

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwekhwalithi yamagciwane okuphefumula ku-nucleic acid ekhishwe kumasampula e-oropharyngeal swab yabantu.

Le modeli isetshenziselwa ukutholwa kwekhwalithi ka-2019-nCoV, igciwane lomkhuhlane A, igciwane lomkhuhlane B kanye ne-respiratory syncytial virus nucleic acids kumasampula e-oropharyngeal swab.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-RT158A Amagciwane Aphefumulayo Ahlanganisiwe Wokuthola Ikhithi(Fluorescence PCR)

Isitifiketi

I-Epidemiology

I-Corona Virus Disease 2019, ebizwa ngokuthi'I-COVID 19', isho inyumoniya ebangelwa ukutheleleka nge-2019-nCoV.I-2019-nCoV iyi-coronavirus yohlobo lwe-β.I-COVID-19 yisifo esithathelwanayo esinamandla sokuphefumula, futhi inani labantu ngokuvamile liyathinteka.Njengamanje, umthombo wokutheleleka ikakhulukazi iziguli ezingenwe yi-2019-nCoV, futhi abantu abangenwe yi-asymptomatic bangase babe umthombo wokutheleleka.Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezingu-1-14, ikakhulukazi izinsuku ezingu-3-7.Umkhuhlane, ukukhwehlela okomile nokukhathala yizimpawu eziyinhloko.Iziguli ezimbalwa zazinezimpawu ezinjengokuminyana emakhaleni, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo, njll.

Umkhuhlane, owaziwa ngokuthi "umkhuhlane", isifo esithathelwanayo sokuphefumula esibangelwa igciwane lomkhuhlane.Iyathelelana kakhulu.Isakazwa kakhulu ngokukhwehlela kanye nokuthimula.Ivamise ukuqubuka entwasahlobo nasebusika.Amagciwane omkhuhlane we-influenza ahlukaniswe abe umkhuhlane A (IFV A), umkhuhlane B (IFV B), kanye no-C (IFV C) izinhlobo ezintathu, zonke zingezamagciwane anamathelayo, abangela isifo somuntu ikakhulukazi kumagciwane omkhuhlane A kanye no-B, iwuhlobo olulodwa. -i-stranded, i-RNA virus ehlukene.Igciwane loMkhuhlane A liwukutheleleka kokuphefumula okunamandla, okuhlanganisa i-H1N1, i-H3N2 nezinye izinhlobo ezincane, ezivame ukuguquka nokuqubuka emhlabeni jikelele.I-"Shift" ibhekisela ekuguqulweni kwegciwane lomkhuhlane A, okuholela ekuveleni kwegciwane elisha "i-subtype".Amagciwane oMkhuhlane B ahlukaniswe izigaba ezimbili, i-Yamagata ne-Victoria.Igciwane lomkhuhlane B line-antigenic drift kuphela, futhi ligwema ukubhekwa nokuqedwa kwamasosha omzimba ngokuguqulwa kwawo.Kodwa-ke, ijubane lokuvela kwegciwane lomkhuhlane B lihamba kancane kunelo legciwane le-human influenza A.Igciwane loMkhuhlane B lingase futhi libangele izifo zokuphefumula komuntu futhi libangele izifo eziwumshayabhuqe.

I-Respiratory syncytial virus (RSV) igciwane le-RNA, eliyingxenye yomndeni we-paramyxoviridae.Idluliselwa ngamaconsi omoya kanye nokuthintana eduze futhi iyi-pathogen eyinhloko yezifo eziphansi zokuphefumula ezinsaneni.Izinsana ezingenwe i-RSV zingase zibe ne-bronchiolitis enamandla nenyumoniya, okuhlobene nesifuba somoya ezinganeni.Izinsana zinezimpawu ezinzima, ezihlanganisa ukushisa okukhulu, i-rhinitis, i-pharyngitis ne-laryngitis, bese kuba yi-bronchiolitis ne-pneumonia.Izingane ezimbalwa ezigulayo zingaba yinkimbinkimbi nge-otitis media, i-pleurisy ne-myocarditis, njll.

Isiteshi

FAM	I-SARS-CoV-2
I-VIC(HEX)	I-RSV
CY5	IFV A
I-ROX	IFV B
I-Quasar 705	Ukulawula Kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji	-18℃
Impilo yeshelufu	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	I-Oropharyngeal swab
Ct	≤38
LoD	2019-nCoV: 300Copies/mL Igciwane lomkhuhlane A/Igciwane loMkhuhlane B/Igciwane le-Respiratory syncytial: 500Copies/mL
Ukucaciswa	a) Imiphumela ye-cross-reactivity ibonisa ukuthi akukho ukusabela okuphambene phakathi kwekhithi ne-coronavirus yabantu i-SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, i-rhinovirus A, B, C, i-chlamydia pneumoniae, i-metapneumovirus yabantu, i-enterovirus A, B, C, D, igciwane le-epstein-barr, igciwane lesimungumungwane, igciwane le-human cytomegalo, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, i-legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, i-smoke aspergillus, candida albicans, candida glabrata, pneumocystis pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, intuthu aspergillus, candida albicans, candida glabrata, pneumocystis pyogenesis entsha futhi cryptococcus genococicus jiroveccus. b) Ikhono lokungaphazanyiswa: khetha i-mucin (60mg/mL), 10% (v/v) yegazi ne-phenylephrine (2mg/mL), i-oxymetazoline (2mg/mL), i-sodium chloride (kuhlanganise nezivimbelakuvunda) (20mg/mL) , i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide (20μg/mL), i-triamcinolone acetonide (2mg/mL), i-budesonide (2mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL) , histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir(500mg/mL) ), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) kanye tobramycin (0.6mg/mL), ) ukuhlolwa kokuphazamiseka, futhi imiphumela ibonisa ukuthi izinto eziphazamisayo ezinokugxila okukhulunywe ngenhla azinakho ukusabela kokuphazamiseka emiphumeleni yokuhlolwa yamagciwane.
Izinsimbi Ezisebenzayo	I-BioRad CFX96 Real-Time PCR System I-Rotor-Gene Q 5plex HRM Platform yesikhathi sangempela PCR System