Izifo Zokuphefumula Ezihlanganisiwe

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola izifo ezibangelwa ukuphefumula ezitholakala ku-nucleic acid ekhishwe kumasampula e-oropharyngeal swab yomuntu.

Le modeli isetshenziselwa ukuthola i-2019-nCoV, igciwane le-influenza A, igciwane le-influenza B kanye ne-nucleic acids yegciwane lokuphefumula elihambisanayo kumasampula e-human oropharyngeal swab.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola Amagciwane Okuphefumula Ehlanganisiwe ye-HWTS-RT158A (i-Fluorescence PCR)

Isitifiketi

I-Epidemiology

Isifo Segciwane Le-Corona 2019, esibizwa ngokuthi'I-COVID 19', ibhekisela ku-pneumonia ebangelwa ukutheleleka ngo-2019-nCoV. I-2019-nCoV iyi-coronavirus eyingxenye yohlobo lwe-β. I-COVID-19 iyisifo esithathelwanayo sokuphefumula esibukhali, futhi abantu ngokuvamile bayathinteka. Njengamanje, umthombo wokutheleleka ikakhulukazi yiziguli ezitheleleke ngo-2019-nCoV, futhi abantu abanegciwane abangenazo izimpawu nabo bangaba umthombo wokutheleleka. Ngokusekelwe ocwaningweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku eziyi-1-14, ikakhulukazi izinsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomile kanye nokukhathala yizimpawu eziyinhloko. Iziguli ezimbalwa zazinezimpawu ezifana nokuminyana kwamakhala, ikhala eligelezayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo, njll.

I-Influenza, eyaziwa kakhulu ngokuthi "i-flu", iyisifo esithathelwanayo sokuphefumula esibangelwa yigciwane le-influenza. Ithelelana kakhulu. Idluliselwa kakhulu ngokukhwehlela nokuthimula. Ivame ukuqubuka entwasahlobo nasebusika. Amagciwane e-Influenza ahlukaniswe izinhlobo ezintathu ze-influenza A (IFV A), i-influenza B (IFV B), kanye ne-Influenza C (IFV C), zonke zingegciwane elinamathelayo, elibangela izifo kubantu ikakhulukazi amagciwane e-influenza A kanye ne-B, iyigciwane le-RNA elinomugqa owodwa, elihlukaniswe ngezigaba. Igciwane le-Influenza A liyigciwane lokuphefumula elinamandla, okuhlanganisa i-H1N1, i-H3N2 kanye nezinye izinhlobo, ezivame ukuguquka nokuqhuma emhlabeni jikelele. Igama elithi "Shift" libhekisela ekuguqukeni kwegciwane le-influenza A, okuholela ekuveleni kwegciwane elisha "elingaphansi". Amagciwane e-Influenza B ahlukaniswe ngezigaba ezimbili, i-Yamagata ne-Victoria. Igciwane le-Influenza B linokuhamba kwe-antigenic kuphela, futhi ligwema ukubhekwa nokususwa kwesistimu yomzimba womuntu ngokuguqulwa kwalo. Kodwa-ke, ijubane lokuvela kwegciwane le-influenza B lihamba kancane kunelegciwane le-influenza A lomuntu. Igciwane le-Influenza B lingabangela nezifo zokuphefumula kubantu futhi liholele ezifweni eziwumshayabhuqe.

Igciwane le-Respiratory syncytial (RSV) liyigciwane le-RNA, elivela emndenini we-paramyxoviridae. Lidluliselwa ngamaconsi omoya kanye nokuxhumana okuseduze futhi liyimbangela eyinhloko yokutheleleka kwepheshana lokuphefumula eliphansi ezinganeni. Izinsana ezinegciwane le-RSV zingase zibe ne-bronchiolitis enzima kanye ne-pneumonia, okuhlobene ne-asthma ezinganeni. Izinsana zinezimpawu ezinzima, okuhlanganisa umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kanye ne-laryngitis, bese kuba yi-bronchiolitis kanye ne-pneumonia. Izingane ezimbalwa ezigulayo zingaba yinkimbinkimbi nge-otitis media, i-pleurisy kanye ne-myocarditis, njll. Ukutheleleka kwepheshana lokuphefumula eliphezulu kuyisibonakaliso esiyinhloko sokutheleleka kubantu abadala kanye nezingane ezindala.

Isiteshi

I-FAM	I-SARS-CoV-2
I-VIC(HEX)	I-RSV
CY5	I-IFV A
I-ROX	I-IFV B
I-Quasar 705	Ukulawula Kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	-18℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	I-Oropharyngeal swab
Ct	≤38
I-LoD	2019-nCoV: Amakhophi angu-300/mL Igciwane le-Influenza A/Igciwane le-Influenza B/Igciwane le-Respiratory syncytial virus: 500 Amakhophi/mL
Ukucaciswa	a) Imiphumela yokusabela okuxubile ikhombisa ukuthi akukho ukusabela okuxubile phakathi kwekhithi kanye ne-human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, i-parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kanye ne-cryptococcus esanda kuzalwa kanye ne-human genomic nucleic acid. b) Amandla okulwa nokuphazamiseka: i-mucin ekhethiwe (60mg/mL), i-10% (v/v) yegazi kanye ne-phenylephrine (2mg/mL), i-oxymetazoline (2mg/mL), i-sodium chloride (kufaka phakathi izilondolozi) (20mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide (20μg/mL), i-triamcinolone acetonide (2mg/mL), i-budesonide (2mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-alpha interferon (800IU/mL), i-zanamivir (20mg/mL), i-ribavirin (10mg/mL), i-oseltamivir (60ng/mL), i-peramivir (1mg/mL), i-lopinavir (500mg/mL), i-ritonavir(60mg/mL), i-mupirocin (20mg/mL), i-azithromycin (1mg/mL), i-ceftriaxone (40μg/mL), i-meropenem (200mg/mL), i-levofloxacin (10μg/mL) kanye ne-tobramycin (0.6mg/mL) ukuze kuhlolwe ukuphazamiseka, futhi imiphumela ikhombisa ukuthi izinto eziphazamisayo ezinamazinga okukhulunywe ngawo ngenhla azinawo ukusabela kokuphazamiseka emiphumeleni yokuhlolwa kwamagciwane.
Izinsimbi Ezisebenzayo	Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-PCR lwe-Rotor-Gene Q 5plex HRM Platform lwesikhathi sangempela