Amagciwane Okuphefumula Ahlanganisiwe
Igama lomkhiqizo
I-HWTS-RT183-Ikhithi Yokuthola Amagciwane Ahlanganisiwe Yokuphefumula (i-Fluorescence PCR)
I-Epidemiology
I-Corona Virus Disease 2019, ebizwa ngokuthi 'i-COVID-19', isho inyumoniya ebangelwa ukutheleleka kwe-SARS-CoV-2. I-SARS-CoV-2 iyi-coronavirus yohlobo lwe-β. I-COVID-19 yisifo esithathelwanayo esinamandla sokuphefumula, futhi inani labantu ngokuvamile liyathinteka. Njengamanje, umthombo wokutheleleka ikakhulukazi iziguli ezingenwe yi-2019-nCoV, futhi abantu abangenwe yi-asymptomatic bangase babe umthombo wokutheleleka. Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezingu-1-14, ikakhulukazi izinsuku ezingu-3-7. Umkhuhlane, ukukhwehlela okomile nokukhathala yizimpawu eziyinhloko. Iziguli ezimbalwa zazinezimpawu ezinjengokuminyana emakhaleni, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo, njll. Umkhuhlane, owaziwa ngokuthi 'umkhuhlane', isifo esithathelwanayo esinamandla sokuphefumula esibangelwa igciwane lomkhuhlane. Iyathelelana kakhulu. Isakazwa kakhulu ngokukhwehlela kanye nokuthimula. Ivamise ukuqubuka entwasahlobo nasebusika. Amagciwane omkhuhlane we-influenza ahlukaniswe abe umkhuhlane A (IFV A), umkhuhlane B (IFV B), kanye no-C (IFV C) izinhlobo ezintathu, zonke zingezamagciwane anamathelayo, abangela isifo somuntu ikakhulukazi kumagciwane omkhuhlane A kanye no-B, iyigciwane le-RNA elinomugqa owodwa, elihlukaniswe izingxenye. Igciwane loMkhuhlane A liwukutheleleka kokuphefumula okunamandla, okuhlanganisa i-H1N1, i-H3N2 nezinye izinhlobo ezincane, ezijwayele ukuguquka nokuqubuka emhlabeni jikelele. I-'Shift' ibhekisela ekuguqulweni kwegciwane lomkhuhlane A, okuholela ekuveleni kwegciwane elisha elithi 'subtype'. Amagciwane oMkhuhlane B ahlukaniswe imigqa emibili, igciwane le-Yamagata kanye ne-Victoria Influenza B line-antigenic drift kuphela, futhi libalekela ukubhekwa nokuqedwa kwesistimu yamasosha omzimba ngokuguqulwa kwawo. Kodwa-ke, ijubane lokuvela kwegciwane lomkhuhlane B lihamba kancane kunelo legciwane le-human influenza A. Igciwane loMkhuhlane B lingase futhi libangele izifo zokuphefumula komuntu futhi libangele izifo eziwumshayabhuqe.
I-Respiratory syncytial virus (RSV) igciwane le-RNA, eliyingxenye yomndeni we-paramyxoviridae. Idluliselwa ngamaconsi omoya kanye nokuthintana eduze futhi iyi-pathogen eyinhloko yezifo eziphansi zokuphefumula ezinsaneni. Izinsana ezingenwe i-RSV zingase zibe ne-bronchiolitis enamandla nenyumoniya, okuhlobene nesifuba somoya ezinganeni. Izinsana zinezimpawu ezinzima, ezihlanganisa ukushisa okukhulu, i-rhinitis, i-pharyngitis ne-laryngitis, bese kuba yi-bronchiolitis ne-pneumonia. Izingane ezimbalwa ezigulayo zingaba yinkimbinkimbi nge-otitis media, i-pleurisy kanye ne-myocarditis, njll.Ukutheleleka kwepheshana eliphezulu lokuphefumula kuyisibonakaliso esiyinhloko sokutheleleka kubantu abadala kanye nezingane ezindala.
Imingcele Yezobuchwepheshe
| Isitoreji | -18℃ ebumnyameni |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | i-oropharyngeal swab; I-Nasopharyngeal swab |
| Ct | I-IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | 200 Amakhophi/μL |
| Ukucaciswa | Imiphumela ye-cross-reactivity ibonisa ukuthi akukho ukusabela okuphambene phakathi kwekhithi ne-cytomegalovirus, i-herpes simplex virus uhlobo 1, i-varicella zoster virus, igciwane le-Epstein-Barr, i-adenovirus, i-human metapneumovirus, i-rhinovirus, i-parainfluenza virus uhlobo I/II/III/IV, bocavirus, enterovirus, coronavirus, Mycoplasmet pneumonia, i-Clasis. I-Corynebacterium spp., i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus spp., i-Legionella pneumophila, i-Moraxella catarrhalis, izinhlobo ezinciphile ze-Mycobacterium tuberculosis, i-Neisseria meningitidis, i-Neisseria spp, i-Neisseria spp. I-Streptococcus pneumoniae, i-Staphylococcus epidermidis, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Acinetobacter baumannii, i-Stenotrophomonas maltophilia, i-Burkholderia cepacia, i-Corynebacterium fasciatum, i-Nocardia, i-Cryptococternscoccus, i-Cryptocternscoccus martophia Aspergillus fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q fever kanye human genomic nucleic acid. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 I-Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad 9 Real-Time PCR System, BioRad 9 Real-Time PCR System. |
Ukugeleza komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-17u-80 esetshenziswa)TM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ivolumu yesampula ekhishiwe ingu-200μL futhi ivolumu ye-lution enconyiwe ingu-150μL.
