Izifo Zokuphefumula Ezihlanganisiwe

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola i-SARS-CoV-2, igciwane le-influenza A, igciwane le-influenza B, igciwane le-influenza A H1N1 kanye nama-nucleic acid e-nucleic virus e-respiratory syncytial kumasampula e-human oropharyngeal swab kanye ne-nasopharyngeal swab.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola Izifo Eziphefumulayo ye-HWTS-RT183 (i-Fluorescence PCR)

I-Epidemiology

Isifo Segciwane Le-Corona sika-2019, esibizwa ngokuthi 'i-COVID-19', sibhekisela e-pneumonia ebangelwa ukutheleleka kwe-SARS-CoV-2. I-SARS-CoV-2 iyi-coronavirus eyingxenye yohlobo lwe-β. I-COVID-19 iyisifo esithathelwanayo sokuphefumula esibukhali, futhi abantu bavame ukungenwa kalula. Njengamanje, umthombo wokutheleleka ikakhulukazi yiziguli ezitheleleke yi-2019-nCoV, futhi abantu abanegciwane abangenazo izimpawu bangaba umthombo wokutheleleka. Ngokusekelwe ocwaningweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku eziyi-1-14, ikakhulukazi izinsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomile kanye nokukhathala yizimpawu eziyinhloko. Iziguli ezimbalwa zazinezimpawu ezifana nokuminyana kwamakhala, ikhala eligelezayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo, njll. Umkhuhlane, owaziwa kakhulu ngokuthi 'umkhuhlane', uyisifo esithathelwanayo sokuphefumula esibukhali esibangelwa yigciwane lomkhuhlane. Uthelelana kakhulu. Udluliselwa kakhulu ngokukhwehlela nokuthimula. Ngokuvamile uqubuka entwasahlobo nasebusika. Amagciwane omkhuhlane ahlukaniswe izinhlobo ezintathu: umkhuhlane A (IFV A), umkhuhlane B (IFV B), kanye nomkhuhlane C (IFV C), wonke angowegciwane elinamathelayo, abangela izifo kubantu ikakhulukazi amagciwane omkhuhlane A no-B, yigciwane le-RNA elinomugqa owodwa, elihlukaniswe ngezigaba. Igciwane le-Influenza A liyigciwane lokuphefumula elibukhali, okuhlanganisa i-H1N1, i-H3N2 nezinye izinhlobo, ezivame ukuguquka nokuqhuma emhlabeni jikelele. Igama elithi 'Shift' libhekisela ekuguqukeni kwegciwane le-influenza A, okuholela ekuveleni 'kwegciwane' elisha. Amagciwane e-Influenza B ahlukaniswe ngezigaba ezimbili, igciwane le-Yamagata ne-Victoria Influenza B linokukhukhuleka kwe-antigenic kuphela, futhi ligwema ukubhekwa nokususwa kwesistimu yomzimba womuntu ngokuguqulwa kwalo. Kodwa-ke, ijubane lokuvela kwegciwane le-influenza B lihamba kancane kunelegciwane le-influenza A lomuntu. Igciwane le-Influenza B lingadala nokutheleleka kokuphefumula kwabantu futhi liholele ezifweni eziwumshayabhuqe.

Igciwane le-Respiratory syncytial (RSV) liyigciwane le-RNA, elivela emndenini we-paramyxoviridae. Lidluliselwa ngamaconsi omoya kanye nokuxhumana okuseduze futhi liyimbangela eyinhloko yokutheleleka kwepheshana lokuphefumula eliphansi ezinganeni. Izinsana ezinegciwane le-RSV zingaba ne-bronchiolitis enzima kanye ne-pneumonia, okuhlobene ne-asthma ezinganeni. Izinsana zinezimpawu ezinzima, okuhlanganisa umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kanye ne-laryngitis, bese kuba yi-bronchiolitis kanye ne-pneumonia. Izingane ezimbalwa ezigulayo zingaba yinkimbinkimbi nge-otitis media, i-pleurisy kanye ne-myocarditis, njll. Ukutheleleka kwepheshana lokuphefumula eliphezulu kuyisibonakaliso esiyinhloko sokutheleleka kubantu abadala kanye nezingane ezindala.

Amapharamitha Obuchwepheshe

Isitoreji	-18℃ Ebumnyameni
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-9
Uhlobo Lwesifanekiso	I-Oropharyngeal swab; I-Nasopharyngeal swab
Ct	I-IFV A, i-IFVB, i-RSV, i-SARS-CoV-2, IFV A H1N1Ct≤38
CV	≤5%
I-LoD	Amakhophi angu-200/mL
Ukucaciswa	Imiphumela yokusabela okuxubile ikhombisa ukuthi akukho ukusabela okuxubile phakathi kwekhithi ne-cytomegalovirus, uhlobo 1 lwegciwane le-herpes simplex, igciwane le-varicella zoster, igciwane le-Epstein-Barr, i-adenovirus, i-human metapneumovirus, i-rhinovirus, igciwane le-parainfluenza uhlobo I/II/III/IV, i-bocavirus, i-enterovirus, i-coronavirus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, i-Bordetella pertussis, i-Corynebacterium spp., i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus spp., i-Legionella pneumophila, i-Moraxella catarrhalis, izinhlobo ezincishisiwe zesifo sofuba se-Mycobacterium, i-Neisseria meningitidis, i-Neisseria spp., i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Streptococcus pneumoniae, i-Staphylococcus epidermidis, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Acinetobacter baumannii, i-Stenotrophomonas i-maltophilia, i-Burkholderia cepacia, i-Corynebacterium fasciatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter rodentium, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis carinii, i-Candida albicans, i-Roseburia mucosa, i-Streptococcus oralis, i-Klebsiella pneumoniae, i-Chlamydia psittaci, i-Rickettsia Q fever kanye ne-human genomic nucleic acid.
Izinsimbi Ezisebenzayo	Izinhlelo ze-Biosystems ezisetshenzisiwe 7500 Real-Time PCR, Izinhlelo ze-Biosystems ezisetshenzisiwe 7500 Fast Real-Time PCR, i-QuantStudio^®Izinhlelo ezi-5 ze-PCR Zesikhathi Sangempela, Izinhlelo ze-SLAN-96P ze-PCR Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), i-LightCycler^®Uhlelo lwe-PCR lwe-480 Real-Time, i-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), i-BioRad CFX96 Real-Time PCR System, i-BioRad CFX Opus 96 Real-Time PCR System.

Ukugeleza Komsebenzi

I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-Eudemon^TMI-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.

Okwedlule: Ikhithi Yokuthola I-Legionella Pneumophila Nucleic Acid

Olandelayo: Izinhlobo eziyi-11 zezifo zokuphefumula ezomisiwe ngeqhwa I-Nucleic Acid

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