I-SARS-CoV-2/umkhuhlane A /umkhuhlane B

Incazelo emfushane:

Le khithi ifanelekela ukutholwa kwe-SARS-CoV-2, i-influenza A kanye ne-influenza B nucleic acid ye-swab ye-nasopharyngeal kanye namasampula e-oropharyngeal swab ukuthi yibaphi abantu ababesolwa ngokutheleleka kwe-SARS-CoV-2, i-influenza A kanye ne-influenza B. Ingasetshenziswa futhi ezimweni ezisolwayo ze-pneumonia kanye nezigameko zeqembu kanye nokutholwa kwe-qualitative kanye nokuhlonza i-SARS-CoV-2, i-influenza A kanye ne-influenza B nucleic acid kumasampula e-nasopharyngeal swab kanye ne-oropharyngeal swab yokutheleleka okusha kwe-Coronavirus kwezinye izimo.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola I-HWTS-RT148-SARS-CoV-2/Umkhuhlane A /Umkhuhlane B I-Nucleic Acid Combined (i-Fluorescence PCR)

Isiteshi

Igama Lesiteshi	I-PCR-Mix 1	I-PCR-Mix 2
Isiteshi se-FAM	I-ORF1ab gene	I-IVA
Isiteshi se-VIC/HEX	Ukulawula kwangaphakathi	Ukulawula kwangaphakathi
Isiteshi se-CY5	I-gene ye-N	/
Isiteshi se-ROX	I-E gene	I-IVB

Amapharamitha Obuchwepheshe

Isitoreji	-18℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	ama-swabs e-nasopharyngeal kanye nama-swabs e-oropharyngeal
Ithagethi	Izinhloso ezintathu ze-SARS-CoV-2 (izakhi zofuzo ze-Orf1ab, N kanye ne-E)/umkhuhlane A /umkhuhlane B
Ct	≤38
CV	≤10.0%
I-LoD	I-SARS-CoV-2: Amakhophi angu-300/mL Igciwane le-influenza A: Amakhophi angu-500/mL Igciwane le-influenza B: Amakhophi angu-500/mL
Ukucaciswa	a) Imiphumela yokuhlolwa okuhlanganisiwe ikhombisile ukuthi ikhithi yayihambisana ne-human coronavirus SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, igciwane lokuphefumula elihambisanayo A no-B, igciwane le-parainfluenza 1, 2 no-3, i-rhinovirusA, B no-C, i-adenovirus 1, 2, 3, 4, 5, 7 no-55, i-human metapneumovirus, i-enterovirus A, B, C no-D, igciwane le-human cytoplasmic pulmonary, igciwane le-EB, igciwane le-measles I-Human cytomegalovirus, i-rotavirus, i-norovirus, igciwane le-mumps, igciwane le-varicella zoster, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, i-Legionella, i-pertussis, i-Haemophilus influenzae, i-Staphylococcus aureus, i-Streptococcus pneumoniae, i-Streptococcus pyogenes, i-Klebsiella pneumoniae, i-Mycobacterium tuberculosis, i-Aspergillus i-fumigatus, i-Candida albicans, i-Candida glabrata Akukho ukusabela okuhlangene phakathi kwe-Pneumocystis yersini kanye ne-Cryptococcus neoformans. b) Amandla okulwa nokuphazamiseka: i-select mucin (60mg/mL), igazi lomuntu elingu-10% (V/V), i-diphenylephrine (2mg/mL), i-hydroxymethylzoline (2mg/mL), i-sodium chloride (equkethe isivikelo) (20mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisone (20μg/mL), i-triamcinolone acetonide (2mg/mL), i-budesonide (2mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-α-Interferon (800IU/mL), i-zanamivir (20mg/mL), i-ribavirin (10mg/mL), i-oseltamivir (60ng/mL), i-pramivir (1mg/mL), i-lopinavir (500mg/mL), i-ritonavir (60mg/mL), i-mupirocin (20mg/mL), i-azithromycin (1mg/mL), i-ceprotene (40μg/mL) i-Meropenem (200mg/mL), i-levofloxacin (10μg/mL) kanye ne-tobramycin (0.6mg/mL). Imiphumela ibonise ukuthi izinto eziphazamisayo ezibalweni ezingenhla azizange ziphazamise imiphumela yokuthola izifo.
Izinsimbi Ezisebenzayo	Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-SLAN^®-96P Izinhlelo ze-PCR Zesikhathi Sangempela Izinhlelo ze-PCR ze-QuantStudio™ ezi-5 zesikhathi sangempela I-LightCycler^®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR