AbakwaSars-Cov-2 / Umkhuhlane A / Umkhuhlane B

Incazelo emfushane:

Le khithi ilungele ukutholwa kwe-vitro okufanelekile kwe-SARS-Cov-2, umkhuhlane a kanye nomkhuhlane acid we-nasopharyngeap acid ye-nasopharyngeap yamasampula okusolwa ukuthi yi-SARS-CoV-2, umkhuhlane a kanye nomkhuhlane B. futhi kungasetshenziswa kuma-pneumonia okusolwa kanye namacala asolwayo okusolwa kanye nokutholwa kokufanelekile nokukhomba AbakwaSars-Cov-2, umkhuhlane a and fucleenzo b nucleic acid e-nasopharyngelling swab kanye namasampula e-oropharyngeal swab atheleleka ngezinye izimo.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-RT148-SARS-COV-2 / Umkhuhlane A / Umkhuhlane B Nucleic Acid I-Kit (Fluorescence PCR)

Insungubezi

Igama lesiteshi	Pcr-mix 1	Pcr-mix 2
Isiteshi se-Fam	Uhlobo lwe-Orf1ab	Ika
Isiteshi seVic / Hex	Ukulawulwa Kwangaphakathi	Ukulawulwa Kwangaphakathi
Isiteshi seCy5	N gene	/
Isiteshi se-ROX	E Uhlobo	I-IVB

Amapharamitha Ezobuchwepheshe

Ukubeka	-18 ℃
Ishalofu-impilo	Izinyanga eziyi-12
Uhlobo lwe-Specimen	I-Nasopharyngeal Swabs kanye nama-Oropharyngengeal Swabs
Okuqondiwe	Abakwa-SARS-COV-2 THORTS 3 (ORF1AB, N no-E no-E / Umkhuhlane A / Umkhuhlane B
Ct	≤38
CV	≤10.0%
Indlu edayisa insimbi	I-SARS-CoV-2: 300 Amakhophi / ml Umkhuhlane Igciwane: Amakhophi angama-500 / ml umkhuhlane b virus: amakhophi angama-500 / ml
Ukucacisa	a) Imiphumela yokuhlolwa kwesiphambano ikhombisa ukuthi ikhithi yayihambisana ne-sarstrman yabantu ye-sarhlanga, i-mersr-cov, i-HCOV-OC43, HCOV-NL63, HCOV-NL63, HOVV-NL63, 2 no-3, Rhinovirusa, B no-C, Adenovirus 1, 2, 3, 4, 5, 7 no-55, Metapneumovirus, i-Elovirus A, B, C no-D, i-Mycoplasma, i-rush virus, i-mycoplasma pneumoniae, Chlambplasma Pneumoniae, ILegionella, I-Pertussis, i-haemophilus yomkhumulo, i-staphylococcus aureus, i-streptococcus pneumoniae, i-strobOcoccus tuumoniae, i-aspeptococcus tuumoniae, i-aspeploboccus fumigatus, uCandida Albicans, awunawo ukusabela kwesiphambano phakathi kwe-pneumocystis I-Yerson kanye ne-CryptoCoccus Neoformans. b) Ikhono lokumelana ne-antinga beclomethasone (20mg / ml), dexamethasone (20mg / ml), I-Flunesisone (20Cinolone Acetonide (2mg / ML), i-Budesonide (2mg / ML), i-MomeTasone (2mg / ml), i-fluticasone (2mg / ml), i-activamine hydrochloride, i-5mg / ml), i-α-interferon (800iu / ml), zanamivir (20mg / ml), i-ribavirin (10mg / ml), i-oseltamivir (60ng / ml), i-pramivir (1mg / ml), i-ritonavir (60mg / ml), i-mupirocin (20mg / ml), i-azithromycin ( I-1mg / ml), CEPREPTENE . Imiphumela ikhombisile ukuthi izinto eziphazamisayo ekugxilweni okungenhla zazingenampendulo yokuphazanyiswa kwemiphumela yokuthola ama-pathogen.
Izinsimbi ezisebenzayo	Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR Isilala^®-96P real-time PCRESS Systems I-QuantStudio ™ 5 Izinhlelo ze-PCR zangempela zesikhathi sangempela I-Lightcycler^®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR