I-Enterococcus engamelani ne-Vancomycin kanye ne-Gene engamelani nemithi
Igama lomkhiqizo
I-HWTS-OT090-I-Enterococcus Engamelani neVancomycin kanye neKithi Yokuthola Izakhi Zofuzo Engamelani Nemithi (i-Fluorescence PCR)
I-Epidemiology
Ukumelana Nemithi kuyaziwa nangokuthi ukumelana nemithi, kubhekisela ekumelaneni kwamagciwane esenzweni semithi yokulwa namagciwane. Uma ukumelana nemithi sekwenzekile, umphumela we-chemotherapy wemithi uzoncishiswa kakhulu. Ukumelana Nemithi kuhlukaniswe ngokumelana kwangaphakathi kanye nokumelana okutholiwe. Ukumelana kwangaphakathi kunqunywa izakhi zofuzo ze-bacterial chromosome, ezidluliselwa esizukulwaneni kuya kwesinye, futhi ngeke zishintshe. Ukumelana okutholiwe kungenxa yokuthi ngemva kokuxhumana nama-antibiotic, amabhaktheriya ashintsha izindlela zawo ze-metabolic ukuze angabulawa ngama-antibiotic.
Izakhi zofuzo zokumelana ne-vancomycin i-VanA ne-VanB ziwukumelana nemithi okutholakalayo, phakathi kwazo i-VanA ikhombisa amazinga aphezulu okumelana ne-vancomycin ne-teicoplanin, i-VanB ikhombisa amazinga ahlukene okumelana ne-vancomycin, futhi iyazwela ku-teicoplanin. I-Vancomycin ivame ukusetshenziswa emtholampilo ukwelapha izifo zamagciwane ezine-Gram-positive, kodwa ngenxa yokuvela kwe-enterococci engamelana ne-vancomycin (VRE), ikakhulukazi i-enterococcus faecalis ne-enterococcus faecium, ebalwa ngaphezu kwama-90%, ilethe izinselele ezintsha ezinkulu ekwelashweni kwezokwelapha. Njengamanje, akukho muthi othize wokulwa namagciwane wokwelapha i-VRE. Ngaphezu kwalokho, i-VRE ingadlulisela nezakhi zofuzo zokumelana nemithi kwamanye ama-enterococci noma amanye amabhaktheriya ane-Gram-positive.
Isiteshi
| I-FAM | I-enterococci engamelana ne-Vancomycin (VRE): I-Enterococcus faecalis kanye ne-Enterococcus faecium |
| I-VIC/HEX | Ukulawula Kwangaphakathi |
| CY5 | i-gene yokumelana ne-vancomycin i-VanB |
| I-ROX | i-gene yokumelana ne-vancomycin i-VanA |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | isikhwehlela, igazi, umchamo noma amakholoni ahlanzekile |
| CV | ≤5.0% |
| Ct | ≤36 |
| I-LoD | 103I-CFU/mL |
| Ukucaciswa | Akukho ukuhlangana kwezifo zokuphefumula ezifana ne-klebsiella pneumoniae, i-acinetobacter baumannii, i-pseudomonas aeruginosa, i-streptococcus pneumoniae, i-neisseria meningitidis, i-staphylococcus aureus, i-klebsiella oxytoca, i-haemophilus influenzae, i-A. junii, i-A. haemolyticus, i-legionella pneumophila, i-escherichia coli, i-pseudomonas fluorescens, i-candida albicans, i-chlamydia pneumoniae, i-respiratory adenovirus, noma amasampula aqukethe ezinye izakhi zofuzo ezingamelana nemithi i-CTX, i-mecA, i-SME, amasampula e-SHV ne-TEM. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time |
Ukugeleza Komsebenzi
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
