I-Yersinia Pestis Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-OT014-Yersinia Pestis Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Yersinia pestis, eyaziwa kakhulu ngokuthi i-Yersinia pestis, izala ngokushesha futhi inamandla amakhulu, okuyibhaktheriya elivamile elibangela izifo phakathi kwamagundane kanye nesifo phakathi kwabantu. Kunezindlela ezintathu eziyinhloko zokudlulisela: ①ukudluliselwa ngesikhumba: ukutheleleka ngesikhumba esonakele kanye nolwelwesi lwamafinyila ngenxa yokuthintana ne-sputum yesiguli kanye nobomvu obuqukethe amagciwane, noma ngesikhumba sesilwane, igazi, inyama, kanye nendle yezimpukane zesifo; ②ukudluliselwa ngendle yokugaya: ukutheleleka ngendle yokugaya ngenxa yokudla izilwane ezingcolile; ③ukudluliselwa ngendle yokuphefumula: isikhwehlela esiqukethe amagciwane, amaconsi noma uthuli olusakazeka ngamaconsi okuphefumula, okubangela ubhubhane phakathi kwabantu. Kube khona ubhubhane olukhulu lwesifo emlandweni wesintu, owokuqala kwaba yi-"Plague of Justinian" ngekhulu lesi-6; kulandelwe yi-"Black Death" eyabulala cishe ingxenye eyodwa kwezintathu yabantu baseYurophu ngekhulu le-14; ubhubhane lwesithathu lwaqala eSifundazweni saseYunnan, eShayina ngekhulu le-19, lwabe selusakazekela eningizimu yeShayina lwasakazekela eHong Kong ngisho nasemhlabeni wonke. Phakathi nalezi zifo ezintathu eziwubhubhane, abantu abangaphezu kwezigidi eziyi-100 balahlekelwa izimpilo zabo.
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/μL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) ye-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ingasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusebenzisa. Ivolumu yokuhlanza enconywayo ingu-80μL.
