Izinhlobo eziyi-11 ze-pathogen yokuphefumula
Igama lomkhiqizo
I-HWTS-RT162A 11 izinhlobo ze-pathogen pathogen nucleic acid detection detection(Fleorescence PCR)
Insungubezi
靶标 | Insungubezi | |||
核酸反应液 a | 核酸反应液 b | 核酸反应液 c | 核酸反应液 D | |
HI | Innyembe | MP | Umlenze | -Yenza |
SP | PA | Bp | / | Cy5 |
Kpn | Tha | I-BPP | I-CPN | Ivupho |
内参 | 内参 | 内参 | 内参 | VIC / Hex |
I-Epidemiology
Ukutheleleka kwezifo zokuphefumula kuyisigaba esibalulekile sezifo eziba yingozi enkulu empilweni yabantu, futhi izifundo zikhombisile ukuthi iningi lezifo zokuphefumula zibangelwa yi-bacterial kanye noma amagciwane amagciwane athola umgcini wesifo, okuholela ekutheni kukhuphuke ubukhali besifo kanye Ukufa, ngakho-ke ukucacisa i-pathogen kuvumela ukwelashwa okuhlosiwe futhi kuthuthukise izinga lokusinda kwesiguli[1,2].
Amapharamitha Ezobuchwepheshe
Ukubeka | ≤-18 ℃ |
Ishalofu-impilo | Izinyanga eziyi-12 |
Uhlobo lwe-Specimen | Sputum |
Ct | Sawubona, SP, KPN, PA, ABA, Smet: CT≤33I-BP, BPP, MP, CPN, umlenze: CT≤38 |
CV | <I-5.0% |
Indlu edayisa insimbi | I-Klebsiella pneumoniae, i-streptococcus pneumoniae, i-haemophilus yomkhumulo jikelele, pseudomonasI-Aeruginosa, i-acinetbacter baumannii, i-stenotrophomonas maltophilia neLegionellaI-Pneumophila: 1000 CFU / ML; I-Bordetella Pertussis ne-bacillus parapertussis: 500 cfu / ml; I-MyCoplasma Pneumoniae kanye ne-Chlamydia Pneumoniae: Amakhophi angama-200 / ml. |
Ukucacisa | Imiphumela yokuhlolwa kwe-Cross-Resivity Test iveze ukuthi kwakungekho ukusabela kwesiphambano phakathi kwale kit ne-cytomegalovirus, i-heamop-barb virus, i-escherella-barter virus, i-acorybacilus, i-ligionella pneumophila , Moraxella I-Catarrhalis, i-Attecouted Grounds of Mycobacterium Tuberculosis, Neisyria Meningitidis, Staphylococcus Aureus, Steptococcus Pydermidis, Steptococcus Pyidermidis, Steptococcus Salivarius, i-Acinet Bauumannii, Stenotrophomonas Maltophilia, Burkholderia Cepacia, Corynebacterium Striatum, Nocardia, Serratia Marscens, Aspergillus Flavus, Pneumocystis Jiroveci, i-Romia Mucilaginosus, Streptococcus Oralis, Klebsiella pneumoniae, Chlamydia Psittaci, Coxiella Burchetii kanye nama-acic womuntu genomic nucleic acid. |
Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection Systems I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-Macro & Micro-Test General DNA / RNA KIT (HWTS-3019) (engasetshenziswa nge-macro & micro-3006r extractor ye-nucroic acid (HWS-3006C, i-HIANGSU Macro & Micro-Test Med-Tech Co, Ltd. Faka i-200μL ye-okuphathelene nomzimbausawoti we-precipitate ecubunguliwe, futhi izinyathelo ezalandela kufanele zenziwe ngokuya ngemiyalo. IVolumu Ye-Exture enconyiwe iyi-100 μl.