Izinhlobo eziyi-11 ze-Respiratory Pathogen
Igama lomkhiqizo
I-HWTS-RT162A Izinhlobo eziyi-11 ze-Respiratory Pathogen Nucleic Acid Detection Kit(I-PCR ye-Fluorescence)
Isiteshi
| 靶标 | Isiteshi | |||
| 核酸反应液A | 核酸反应液B | 核酸反应液C | 核酸反应液D | |
| HI | I-Smet | MP | Umlenze | I-FAM |
| SP | PA | Bp | / | CY5 |
| I-KPN | I-ABA | I-Bpp | I-Cpn | I-ROX |
| 内参 | 内参 | 内参 | 内参 | I-VIC/HEX |
I-Epidemiology
Ukutheleleka kwezifo zokuphefumula kuyisigaba esibalulekile sezifo ezisongela impilo yabantu, futhi izifundo zibonise ukuthi izifo eziningi zokuphefumula zibangelwa amagciwane athelela ndawonye kumuntu ophethe lesi sifo, okuholela ekwandeni kobunzima besifo ngisho nokufa, ngakho-ke ukucacisa igciwane kuvumela ukwelashwa okuqondiwe futhi kuthuthukisa izinga lokusinda kwesiguli.[1,2].
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isikhwehlela |
| Ct | HI、SP、KPN、PA、ABA、Smet：Ct≤33I-BP、Bpp、MP、Cpn、Leg：Ct≤38 |
| CV | <5.0% |
| I-LoD | Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonasi-aeruginosa, i-Acinetobacter baumannii, i-Stenotrophomonas maltophilia kanye ne-Legionellai-pneumophila: 1000 CFU/mL； I-Bordetella pertussis kanye ne-Bacillus parapertussis: 500 CFU/mL; I-Mycoplasma pneumoniae kanye ne-Chlamydia pneumoniae: amakhophi angu-200/mL. |
| Ukucaciswa | Imiphumela yokuhlolwa kwe-cross-reactivity ibonise ukuthi akukho ukusabela okuhlangene phakathi kwale kit ne-Cytomegalovirus, uhlobo lwegciwane le-Herpes simplex 1, igciwane le-Varicella-zoster, igciwane le-Epstein-Barr, i-Bordetella pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella pneumophila, i-Moraxella catarrhalis, izinhlobo ezincishisiwe zesifo sofuba se-Mycobacterium, i-Neisseria meningitidis, i-Neisseria, i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Staphylococcus epidermidis, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Acinetobacter baumannii, i-Stenotrophomonas maltophilia, i-Burkholderia cepacia, i-Corynebacterium striatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis jiroveci, Ama-Candida albicans, i-Rothia mucilaginosus, i-Streptococcus oralis, i-Klebsiella pneumoniae, i-Chlamydia psittaci, i-Coxiella burnetii kanye nama-human genomic nucleic acids. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Engeza u-200µL wengokomzimbaFaka usawoti ku-precipitate ecutshunguliwe, futhi izinyathelo ezilandelayo kufanele zenziwe ngokuhambisana nemiyalelo. Ivolumu yokuhlanza enconywayo ingu-100 µL.
