Izinhlobo eziyi-11 zePathogen yokuphefumula
Igama lomkhiqizo
I-HWTS-RT162A 11 Izinhlobo Zekhithi Yokuthola I-Pathogen Nucleic Acid Yokuphefumula(Fluorescence PCR)
Isiteshi
| 靶标 | Isiteshi | |||
| 核酸反应液A | 核酸反应液B | 核酸反应液C | 核酸反应液D | |
| HI | Smet | MP | Umlenze | FAM |
| SP | PA | Bp | / | CY5 |
| I-KPN | ABA | I-Bpp | Cpn | I-ROX |
| 内参 | 内参 | 内参 | 内参 | VIC/HEX |
I-Epidemiology
Izifo zokuphefumula ziyisigaba esibalulekile sezifo ezibeka usongo olukhulu empilweni yomuntu, futhi ucwaningo luye lwabonisa ukuthi izifo eziningi zokuphefumula zibangelwa amagciwane noma ama-virus noma amagciwane ahlasela umsingathi, okuholela ekwandeni kobunzima besifo ngisho nokufa, ngakho ukucacisa i-pathogen kuvumela ukwelashwa okuhlosiwe futhi kuthuthukisa izinga lokusinda kwesiguli.[1,2].
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isikhwehlela |
| Ct | HI,SP,KPN,PA,ABA,Smet:Ct≤33BP,Bpp,MP,Cpn,Leg:Ct≤38 |
| CV | <5.0% |
| LoD | Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonasi-aeruginosa, i-Acinetobacter baumannii, i-Stenotrophomonas maltophilia kanye ne-Legionellai-pneumophila: 1000 CFU/mL; I-Bordetella pertussis ne-Bacillus parapertussis: 500 CFU/mL; I-Mycoplasma pneumoniae ne-Chlamydia pneumoniae: amakhophi angu-200/mL. |
| Ukucaciswa | Imiphumela yokuhlolwa kwe-cross-reactivity ibonise ukuthi kwakungekho ukusabela okuphambene phakathi kwale kit kanye ne-Cytomegalovirus, i-Herpes simplex virus uhlobo 1, i-Varicella-zoster virus, i-Epstein-Barr virus, i-Bordetella pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella-zoster, i-capital philaslauxelle I-Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Cocomalcoccus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Cocomal bacterium Burkteria i-striatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis jiroveci, i-Candida albicans, i-Rothia mucilaginosus, i-Streptococcus oralis, i-Klebsiella pneumoniae, i-Klebsiella pneumoniae, i-Chlatimic nucleic acid i-Colamydia pneumoniae. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co.,µ0 Engeza L20ngokwempilousawoti ku-precipitate ecutshunguliwe, futhi izinyathelo ezilandelayo kufanele zenziwe ngokuhambisana nemiyalelo. Ivolumu ye-elution enconyiwe ngu-100 µL.
