Izinhlobo eziyi-14 zamagciwane okuphefumula ahlanganisiwe
Igama lomkhiqizo
I-HWTS-RT159B 14 Izinhlobo zama-pathogens okuphefumula ahlanganiswe ikhithi yokuthola i-nucleic acid (i-fluescence PCR)
I-Epidemiology
Ukutheleleka kwe-Respiratory Tract yisifo esivame kakhulu kubantu, esingenzeka kunoma ibuphi ubulili, ubudala nesifunda. Ungenye yezimbangela eziholayo zokulimaza nokufa kwabantu emhlabeni wonke[1]. Amagciwane ajwayelekile wokuphefumula afaka i-novel coronavirus, umkhuhlane wegciwane, igciwane le-virus, igciwane lokuphefumula, i-adenovirus, i-parainflueumovirus, i-bocavirus, i-antoovirus, i-coronavirus, i-chlamydia pneumoniae, kanye ne-streptococcus pneumoniae, njll[2,3].
Insungubezi
| Kahle | IGAMA LOKUXHUMANA | Ama-pathogen azotholwa |
| 1 | Hlanganisa kahle a | AbakwaSars-Cov-2, i-IFV A, IFV B |
| 2 | Master Mix B | Adv, HMPV, MP, CPN |
| 3 | Master Mix C | I-PIVI / II / III / IV, RHV, RSV, HBOV |
| 4 | Master Mix d | Cov, eV, SP, Ukulawulwa Kwangaphakathi |
| 5 | Hlanganisa kahle a | AbakwaSars-Cov-2, i-IFV A, IFV B |
| 6 | Master Mix B | Adv, HMPV, MP, CPN |
| 7 | Master Mix C | I-PIVI / II / III / IV, RHV, RSV, HBOV |
| 8 | Master Mix d | Cov, eV, SP, Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | I-Oropharyngel Swab, i-Nasopharyngel Swap |
| Ct | ≤38 |
| CV | <I-5.0% |
| Indlu edayisa insimbi | 200copies / ml |
| Ukucacisa | Imiphumela yokuhlolwa kwe-Cross-Resivity Test iveze ukuthi kwakungekho ukusabela kwesiphambano phakathi kwale kit ne-cytomegalovirus, i-heamop-barb virus, i-escherella-barter virus, i-acorybacilus, i-ligionella pneumophila , Moraxella I-Catarrhalis, i-Attecouted Grounds of Mycobacterium Tuberculosis, Neisyria Meningitidis, Staphylococcus Aureus, Steptococcus Pydermidis, Steptococcus Pyidermidis, Steptococcus Salivarius, i-Acinet Bauumannii, Stenotrophomonas Maltophilia, Burkholderia Cepacia, Corynebacterium Striatum, Nocardia, Serratia Marscens, Aspergillus Flavus, Pneumocystis Jiroveci, i-Romia Mucilaginosus, Streptococcus Oralis, Klebsiella pneumoniae, Chlamydia Psittaci, Coxiella Burchetii kanye nama-acic womuntu genomic nucleic acid. |
| Izinsimbi ezisebenzayo | ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.)Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real I-LineGene 9600 kanye nezinhlelo zokutholwa kwe-PRCR yesikhathi sangempela (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR, Biorad CFX Opus 96 Uhlelo lwe-Real-Time PCR |
Ukugeleza komsebenzi
I-reaglent enconyiwe ye-realent: I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3017) (engasetshenziswa nge-macro & micro-eq Co, Ltd. Ivolumu yesampula ekhishwe i-200μL. Izinyathelo ezilandelayo kufanele zenziwe ngokuya ngemiyalo yokusebenzisa le mpendulo ye-extraction. IVolumu Ye-Expeated Enconyiwe80μl.
