Izinhlobo eziyi-14 zezifo zokuphefumula ezihlanganisiwe
Igama lomkhiqizo
I-HWTS-RT159B Izinhlobo eziyi-14 ze-Respiratory Pathogens Combined Nucleic Acid Detection Kit (i-Fluorescence PCR)
I-Epidemiology
Ukutheleleka kwemigudu yokuphefumula yisifo esivame kakhulu kubantu, esingavela kunoma yiluphi ubulili, ubudala kanye nesifunda. Singenye yezimbangela eziphambili zokugula nokufa kubantu emhlabeni jikelele.[1]. Izifo ezivamile zokuphefumula zifaka phakathi i-coronavirus entsha, igciwane le-Influenza A, igciwane le-Influenza B, igciwane le-Respiratory syncytial, i-Adenovirus, i-human metapneumovirus, i-rhinovirus, igciwane le-Parainfluenza uhlobo lwe-I/II/III/IV, i-Bocavirus, i-Enterovirus, i-Coronavirus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, kanye ne-Streptococcus pneumoniae, njll.[2,3].
Isiteshi
| Indawo Yomthombo | Igama Lesixazululo Sokusabela | Amagciwane Okumele Atholakale |
| 1 | Ingxube Eyinhloko A | I-SARS-CoV-2, i-IFV A, i-IFV B |
| 2 | I-Master Mix B | Ummeli, i-hMPV, i-MP, i-Cpn |
| 3 | I-Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | I-Master Mix D | I-CoV, i-EV, i-SP, ukulawula kwangaphakathi |
| 5 | Ingxube Eyinhloko A | I-SARS-CoV-2, i-IFV A, i-IFV B |
| 6 | I-Master Mix B | Ummeli, i-hMPV, i-MP, i-Cpn |
| 7 | I-Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | I-Master Mix D | I-CoV, i-EV, i-SP, ukulawula kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | I-Oropharyngeal swab, i-Nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | 2Amakhophi angu-00/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kwe-cross-reactivity ibonise ukuthi akukho ukusabela okuhlangene phakathi kwale kit ne-Cytomegalovirus, uhlobo lwegciwane le-Herpes simplex 1, igciwane le-Varicella-zoster, igciwane le-Epstein-Barr, i-Bordetella pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella pneumophila, i-Moraxella catarrhalis, izinhlobo ezincishisiwe zesifo sofuba se-Mycobacterium, i-Neisseria meningitidis, i-Neisseria, i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Staphylococcus epidermidis, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Acinetobacter baumannii, i-Stenotrophomonas maltophilia, i-Burkholderia cepacia, i-Corynebacterium striatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis jiroveci, Ama-Candida albicans, i-Rothia mucilaginosus, i-Streptococcus oralis, i-Klebsiella pneumoniae, i-Chlamydia psittaci, i-Coxiella burnetii kanye nama-human genomic nucleic acids. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.)Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 Real-Time PCR |
Ukugeleza Komsebenzi
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Umthamo wesampula okhishwe ungama-200µL. Izinyathelo ezilandelayo kufanele zenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhipha. Umthamo wokususa onconyiwe ngu80µL.
