Izinhlobo ezingu-14 Zamagciwane Okuphefumula Ahlanganisiwe
Igama lomkhiqizo
I-HWTS-RT159B 14 Izinhlobo Zamagciwane Aphefumulayo Ahlanganiswe Ikhithi Yokubona I-Nucleic Acid (Fluorescence PCR)
I-Epidemiology
Ukutheleleka komgudu wokuphefumula yisifo esivame kakhulu kubantu, esingenzeka kunoma yibuphi ubulili, ubudala kanye nesifunda. Ingenye yezimbangela eziphambili zokugula nokufa kwabantu emhlabeni wonke[1]. Amagciwane avamile okuphefumula ahlanganisa inoveli i-coronavirus, igciwane lomkhuhlane A, igciwane loMkhuhlane B, i-Respiratory syncytial virus, i-Adenovirus, i-human metapneumovirus, i-rhinovirus, i-Parainfluenza virus uhlobo I/II/III/IV, i-Bocavirus, i-Enterovirus, iCoronavirus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, St.[2,3].
Isiteshi
| Well Position | Igama Lesixazululo Sokusabela | Amagciwane Azotholakala |
| 1 | I-Master Mix A | I-SARS-CoV-2, IFV A, IFV B |
| 2 | I-Master Mix B | I-Adv, i-hMPV, i-MP, i-Cpn |
| 3 | I-Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | I-Master Mix D | I-CoV, i-EV, i-SP, ukulawula kwangaphakathi |
| 5 | I-Master Mix A | I-SARS-CoV-2, IFV A, IFV B |
| 6 | I-Master Mix B | I-Adv, i-hMPV, i-MP, i-Cpn |
| 7 | I-Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | I-Master Mix D | I-CoV, i-EV, i-SP, ukulawula kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | I-Oropharyngeal swab, i-Nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Amakhophi/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kwe-cross-reactivity ibonise ukuthi kwakungekho ukusabela okuphambene phakathi kwale kit kanye ne-Cytomegalovirus, i-Herpes simplex virus uhlobo 1, i-Varicella-zoster virus, i-Epstein-Barr virus, i-Bordetella pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella-zoster, i-capital philaslauxelle I-Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Cocomalcoccus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Cocomal bacterium Burkteria i-striatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis jiroveci, i-Candida albicans, i-Rothia mucilaginosus, i-Streptococcus oralis, i-Klebsiella pneumoniae, i-Klebsiella pneumoniae, i-Chlatimic nucleic acid i-Colamydia pneumoniae. |
| Izinsimbi Ezisebenzayo | I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.)I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 ye-Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Isampula ekhishiwe engu-20 L. Izinyathelo ezilandelayo kufanele zenziwe ngokuya ngemiyalelo yokusetshenziswa kwalesi reagent yokukhipha. Ivolumu ye-elution enconyiwe ingu80µL.
