I-Adenovirus Universal
Igama lomkhiqizo
I-HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Human adenovirus (HAdV) ingeyohlobo lwe-Mammalian adenovirus, okuyigciwane le-DNA elinemicu emibili elingenayo imvilophu. Ama-Adenoviruses atholakale kuze kube manje ahlanganisa ama-subgroups angu-7 (AG) kanye nezinhlobo ezingu-67, lapho ama-serotypes angu-55 angama-pathogenic kubantu. Phakathi kwazo, okungase kuholele ezifweni zepheshana lokuphefumula ikakhulukazi iqembu B (Izinhlobo 3, 7, 11, 14, 16, 21, 50, 55), Iqembu C (Izinhlobo 1, 2, 5, 6, 57) kanye neQembu E (Uhlobo 4), futhi kungaholela esihudo samathumbu (Uhlobo 1) [Uhlobo 1] Izinhlobo ezahlukene zinezimpawu zomtholampilo ezihlukene, kodwa ikakhulukazi izifo zepheshana lokuphefumula. Izifo zokuphefumula ezibangelwa izifo zemigudu yokuphefumula emzimbeni womuntu zibalelwa ku-5% ~ 15% wezifo zokuphefumula zomhlaba wonke, kanye no-5% -7% wezifo zokuphefumula zezingane emhlabeni jikelele[9]. I-Adenovirus itholakala ezindaweni eziningi futhi ingatheleleka unyaka wonke, ikakhulukazi ezindaweni eziminyene, ezivame ukuqubuka endaweni, ikakhulukazi ezikoleni nasemakamu ezempi.
Isiteshi
| FAM | i-adenovirus jikelelei-nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-Nasopharyngeal swab,I-swab yomphimbo |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300Amakhophi/mL |
| Ukucaciswa | a) Hlola izinkomba ezingezinhle zenkampani ngokwekhithi, futhi umphumela wokuhlola uhlangabezana nezidingo. b) Sebenzisa le khithi ukuthola futhi akukho ukuhlangana kabusha namanye amagciwane okuphefumula (afana negciwane Lomkhuhlane A, Igciwane Lomkhuhlane B, I-Respiratory syncytial virus, i-Parainfluenza virus, i-Rhinovirus, i-Human metapneumovirus, njll.) noma amagciwane (Streptococcus pneumoniae, Klebcinespneumoniaacmonaella, Plebsiecoccus, Plebsiecoccus, Plebsiecoccus, Pneumoviruse baumannii, Staphylococcus aureus, njll.). |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time LineGene 9600 Plus Real-Time PCR Uhlelo Lokutholas (I-FQD-96A, HangzhouUbuchwepheshe be-Bioer) I-MA-6000 ye-Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR Systems, BioRad CFX Opus 96 Real-Time PCR Systems |
Ukugeleza komsebenzi
(1) I-reagent yokukhipha enconyiwe:I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Ukukhishwa kufanele kwenziwe ngokulandela imiyalelo. Isampula ekhishiwe yiziguli'i-nasopharyngeal swab noma amasampula e-throat swab aqoqwe esizeni. Engeza amasampula kumshini wokukhipha isampula owenziwe yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., i-vortex ukuze uxubane kahle, ubeke ezingeni lokushisa elilingana negumbi imizuzu emi-5, ukhiphe bese uphendulela futhi uhlanganise kahle ukuze uthole i-DNA yesampula ngayinye.
(2) I-reagent yokukhipha enconyiwe:I-Macro & Micro-Test Viral Ikhithi ye-DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).ukusebenza kufanele kwenziwe ngokuhambisana ngokuqinile nemiyalelo. Umthamo wesampula okhishiwe ungama-200μL, kanyeivolumu ye-lution enconyiweis80μL.
(3) I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP315) ngu-Tiangen Biotech (Beijing) Co., Ltd.,ubeukusebenza kufanele kwenziwe ngokuhambisana ngokuqinile nemiyalelo. Umthamo wesampula okhishiwe ungama-200μL, kanyeivolumu ye-lution enconyiweis80μL.
