I-Adenovirus Universal
Igama lomkhiqizo
I-HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Adenovirus yomuntu (i-hadv) ingeye-genus mammalian adenovirus, okuyinto eyigciwane le-DNA elahliwe kabili ngaphandle kwemvilophu. Ama-Adenoviruses atholakele kuze kube manje afaka ama-subgroups ayi-7 (ag) nezinhlobo ezingama-65, lapho ama-serotypes angama-55 anama-pathogenic kubantu. Phakathi kwazo, kungaholela ekutheni izifo zepheshana lokuphefumula ziyiqembu B (izinhlobo 3, 7, 11, 16, 21, 50, 2, 5, 5, 5, 2, 5, 5, 6, 57) neQembu E . Izinhlobo ezahlukahlukene zinezimpawu ezahlukahlukene zomtholampilo, kepha ikakhulukazi izifo zokuphefumula. Izifo zokuphefumula ezibangelwa ukutheleleka kwezifo zokuphefumula ze-akhawunti yomzimba womuntu nge-5% ~ 15% yezifo zokuphefumula komhlaba wonke, kanye ne-5% -7% yezifo zokuphefumula ebuntwaneni bezingane [9]. I-Adenovirus ingukuphela kwezindawo ezahlukahlukene futhi ingatheleleka unyaka wonke, ikakhulukazi ezindaweni ezinabantu abaningi, ezithambekele ekuqubukeni kwasendaweni, ikakhulukazi ezikoleni nasezempi zamasosha.
Insungubezi
| -Yenza | I-Adenovirus UniversalI-nucleic acid |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Nasopharyngel swab,UTroat Swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | 300copies / ml |
| Ukucacisa | a) Hlola izinkomba ezingezinhle zenkampani ezingezinhle ngekhithi, futhi umphumela wokuhlola uhlangabezana nezidingo. b) Sebenzisa leli khithi ukuthola futhi akukho gciwane lokuphindaphindeka kwamanye amagciwane okuphefumula (okufana nomkhuhlane wegciwane, igciwane lokuphefumula, igciwane lomuntu, i-bhertococcus pneumoniae, Klebsiella pneumoniae, pseudomonas I-Aeruginosa, i-acinetbacter baumannii, staphylococcus aureus, njll.). |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection Systems (I-FQD-96A, HangzhouUbuchwepheshe be-bioer) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Systems real-time PCR, biorad CFX Opus 96 Systems real-time PCREMS |
Ukugeleza komsebenzi
(1) I-reaglent enconyiwe ye-reagent:I-Macro & Micro-Test Samputer Supder Reled Reagent (HWS-3005-8). Ukukhishwa kufanele kwenziwe ngokuya ngemiyalo. Isampula elikhishwe yiziguli'Amasampula we-nasopharyngeal swab noma umphimbo we-Swab aqoqwe esizeni. Faka amasampula kwisampula yokukhishwa kwesampula nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. isampula ngayinye.
(2) I-reaglent enconyiwe ye-realent:I-Macro & Micro-Test Uhlobo lwegciwane I-DNA / RNA KIT(I-HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-macro & micro-test automatic athotshisi i-nucleic acid extractor (HWS-3006C, HWS-3006B).Ukuhlinzwa kufanele kwenziwe ngokuhambisana nemiyalo. IVolumu Yesampula Ekhishwe Ama-200μl, noUmthamo wokuqedwa onconyiweisI-80μL.
(3) I-reaglent enconyiwe ye-reaglent: ukukhishwa kwe-nucleic acid noma i-reagent yokuhlanza (ydp315) nguTiangen Biotech (Beijing) Co, Ltd., TheUkusebenza kufanele kwenziwe ngokuhambisana nemiyalo. IVolumu Yesampula Ekhishwe Ama-200μl, noUmthamo wokuqedwa onconyiweisI-80μL.
