I-Adenovirus Universal
Igama lomkhiqizo
Ikhithi Yokuthola I-Adenovirus Universal Nucleic Acid (i-Fluorescence PCR) ye-HWTS-RT017A Adenovirus Universal Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-adenovirus yomuntu (HAdV) ingeyohlobo lwe-Mammalian adenovirus, okuyigciwane le-DNA elinemigqa emibili elingenayo i-envelope. Ama-Adenovirus atholakale kuze kube manje afaka amaqembu amancane angu-7 (AG) kanye nezinhlobo ezingu-67, lapho ama-serotype angu-55 ebangela izifo kubantu. Phakathi kwawo, okungaholela ekuthelelekeni kwendlela yokuphefumula ikakhulukazi yiqembu B (Izinhlobo 3, 7, 11, 14, 16, 21, 50, 55), Iqembu C (Izinhlobo 1, 2, 5, 6, 57) kanye neQembu E (Uhlobo 4), futhi okungaholela ekuthelelekeni kwesifo sohudo emathunjini yiQembu F (Izinhlobo 40 kanye no-41)[1-8]. Izinhlobo ezahlukene zinezimpawu ezahlukene zezokwelapha, kodwa ikakhulukazi izifo zendlela yokuphefumula. Izifo zokuphefumula ezibangelwa izifo zendlela yokuphefumula zomzimba womuntu zingu-5% ~ 15% wezifo zokuphefumula zomhlaba wonke, kanye no-5% -7% wezifo zokuphefumula zezingane emhlabeni jikelele[9]. I-Adenovirus isakazeke ezindaweni eziningi futhi ingatheleleka unyaka wonke, ikakhulukazi ezindaweni ezinabantu abaningi, ezivame ukuba nobhubhane lwendawo, ikakhulukazi ezikoleni nasemakamu ezempi.
Isiteshi
| I-FAM | i-adenovirus jikelelei-nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-nasopharyngeal swab，I-swab yomphimbo |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-300/mL |
| Ukucaciswa | a) Hlola izinkomba ezingezinhle zenkampani ezijwayelekile ngekhithi, bese umphumela wokuhlolwa uhlangabezana nezidingo. b) Sebenzisa le khithi ukuthola futhi akukho ukuhlangana nezinye izifo zokuphefumula (njengegciwane le-Influenza A, igciwane le-Influenza B, igciwane le-Respiratory syncytial, igciwane le-Parainfluenza, i-Rhinovirus, i-Human metapneumovirus, njll.) noma amabhaktheriya (i-Streptococcus pneumoniae, i-Klebsiella pneumoniae, i-Pseudomonas aeruginosa, i-Acinetobacter baumannii, i-Staphylococcus aureus, njll.). |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCRs (I-FQD-96A, eHangzhouUbuchwepheshe be-Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Izinhlelo ze-PCR ze-BioRad CFX96 Real-Time, Izinhlelo ze-PCR ze-BioRad CFX Opus 96 Real-Time |
Ukugeleza Komsebenzi
(1) I-reagent yokukhipha enconyiwe:I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8). Ukukhishwa kufanele kwenziwe ngokwemiyalelo. Isampula ekhishwe yiziguli'amasampula e-nasopharyngeal swab noma e-throat swab aqoqwe endaweni. Faka amasampula ku-release release release reagent yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex ukuze uxube kahle, uwabeke ekamelweni lokushisa imizuzu emi-5, uwakhiphe bese uwaguqula bese uwaxuba kahle ukuze uthole i-DNA yesampula ngayinye.
(2) I-reagent yokukhipha enconyiwe:Ukuhlolwa Okukhulu Nokuncane Okusabalele Ikhithi ye-DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).umsebenzi kufanele wenziwe ngokuhambisana nemiyalelo. Umthamo wesampula okhishwe ungama-200μL, kanyeivolumu yokuxazulula enconyiweis80μL.
(3) I-reagent yokukhipha enconywayo: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP)315) yiTiangen Biotech (Beijing) Co.,Ltd., iumsebenzi kufanele wenziwe ngokuhambisana ngqo nemiyalelo. Umthamo wesampula okhishwe ungama-200μL, kanyeivolumu yokuxazulula enconyiweis80μL.
