I-Carbapenem Ukumelana noGene (KPC / NDM / OXA 48 / Oxa 23 / Vim / Imp)
Igama lomkhiqizo
I-HWTS-OT045 Carbapenem Ukumelana (KPC / NDM / OXA 48 / Oxa 23 / Vim / Imp) Kit (Fluorescence PCR)
I-Epidemiology
I-Carbapenem antibiotics yi-adpical β-lactam antibiotic ene-antibacterial dectrum ebanzi kanye nomsebenzi oqinile we-antibacterial. Ngenxa yokuqina kwayo ku-β-lactamase kanye nobuthi obuphansi, sekusenye yezidakamizwa ezibaluleke kakhulu ze-antibacterial zokwelashwa kwezifo ezinzima zamagciwane. I-Carbapenems zizinzile kakhulu ku-plasmid-mediated enwetshiwe-spectrum β-lactamases (ama-esbls), ama-chromosomes, kanye nama-cephalosporinases (ampc enzyme).
Insungubezi
| Pcr-mix 1 | Pcr-mix 2 | |
| -Yenza | Ind | -Yi-vim |
| VIC / Hex | Ukulawulwa Kwangaphakathi | Ukulawulwa Kwangaphakathi |
| Cy5 | Ndm | I-KPC |
| Ivupho | I-OXA48
| I-OXA23 |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | I-Sputum, amakoloni amsulwa, i-swab ye-rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | 103CFU / ML |
| Ukucacisa | a) Ikhithi ithola izinkomba ezingezinhle zenkampani emibi, futhi imiphumela ihlangabezana nezidingo zezinkomba ezihambisanayo. b) imiphumela yokuhlolwa kwe-cross-reviturity show ukuthi le khithi ayinakusabela kwamanye ama-pathogens okuphefumula, i-anistetomonas aeruginosa, i-streptococcus pneumoniae, i-stroptococcus pneumoniae, i-steptococcus meningitidis, staphylococcus aureus, klebsiella aretoca, I-Haemophilus Umkhuhlane, i-Acinet Junii, i-AcinetLacla Pneumolyticus, EscherIchia Coli, ama-alvudan we-adenoonia, i-entenovoccus, noma amasampula aqukethe ezinye izinhlobo zofuzo ezingamelana nezidakamizwa, iMeca, SME, SHV, TEM, njll. c) Ukuphazamiseka: I-MinCincline, I-Minocycline, Gengane Colipemicin, Clindamycin, Cleforpem, COXROPOXACIN HYDROCHLORIDE, futhi imiphumela ikhombisa ukuthi izinto eziphazamisayo ezingenhla azinakuphazamisa ukusabela ekutholweni kwe I-Carbapenem Ukumelana nohlobo lwe-Carbapenem, NDM, OXA48, OXA23, VIM, ne-Imp. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS I-QuantStudio®5 Systems real-time PCREMS ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 kanye nohlelo lwe-Real-time PCCRECCEED SYPEL (I-FQD-96A,I-hangzhouUbuchwepheshe be-bioer) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
I-reaglent enconyiwe ye-realent: Macro & Micro-Test General DNA / RNA KIT (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (engasetshenziswa nge-macro & micro-test automatic actoult extractor (HWS-3006C, HWS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd .. Faka i-200μL ye-saline ejwayelekile kuyo i-thallus iqonde. Izinyathelo ezalandela kufanele zilandele imiyalo yokukhishwa, futhi ivolumu yokuqedwa elinconyiwe100μl.
Inketho 2.
I-realent enconyiwe I-realent: Ukukhishwa kwe-nucleic acid noma i-realegent yokuhlanza (ydp302) nguTiangen Biotech (Beijing) Co, Ltd. , bese unikina kuze kumiswe imisiwe ngokuphelele). Sebenzisa amanzi we-rnase / DNAse mahhala ukuze uqede, futhi ivolumu yokuqedwa kwe-ecommied is 100μL.
Inketho 3.
I-reaglent enconyiwe ye-realent: I-Macro & Micro-Test Sampula Sampula Sampula Reagent. Isampula ye-sputum idinga ukugezwa ngokungeza i-1ml yosawoti ojwayelekile ophathwe ngenhla kwe-tartipitate, centrifug Ikolo yekoloni elihlanzekile kanye ne-swab elihlanzekile, engeza i-50μL ye-reagent ekhokhelwa isampula ngqo kulolu hlelo olushiwo ngenhla, futhi izinyathelo ezilandelayo kufanele zikhishwe ngokuya ngomyalo wokusetshenziswa.
