I-Gene Yokumelana Ne-Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) (i-Fluorescence PCR)
I-Epidemiology
Ama-antibiotic e-Carbapenem angama-antibiotic angavamile e-β-lactam ane-spectrum ebanzi kakhulu yokulwa namagciwane kanye nomsebenzi onamandla kakhulu wokulwa namagciwane. Ngenxa yokuzinza kwayo ku-β-lactamase kanye nobuthi obuphansi, isibe ngomunye wemithi ebaluleke kakhulu yokulwa namagciwane yokwelapha izifo ezibangelwa amagciwane ezinzima. Ama-Carbapenems azinzile kakhulu kuma-β-lactamases anwetshiwe axhunywe yi-plasmid (ama-ESBL), ama-chromosome, kanye nama-cephalosporinases axhunywe yi-plasmid (ama-enzyme e-AmpC).
Isiteshi
| I-PCR-Mix 1 | I-PCR-Mix 2 | |
| I-FAM | I-IMP | I-VIM |
| I-VIC/HEX | Ukulawula Kwangaphakathi | Ukulawula Kwangaphakathi |
| CY5 | I-NDM | I-KPC |
| I-ROX | I-OXA48
| I-OXA23 |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isikhwehlela, amakholoni ahlanzekile, i-rectal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| I-LoD | 103I-CFU/mL |
| Ukucaciswa | a) Ikhithi ithola izinkomba ezingezinhle zenkampani ezijwayelekile, futhi imiphumela ihlangabezana nezidingo zezinkomba ezifanele. b) Imiphumela yokuhlolwa kwe-cross-reactivity ikhombisa ukuthi le khithi ayinayo impendulo ehambisanayo nezinye izifo zokuphefumula, njenge-Klebsiella pneumoniae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa, i-Streptococcus pneumoniae, i-Neisseria meningitidis, i-Staphylococcus aureus, i-Klebsiella oxytoca, i-Haemophilus influenzae, i-Acinetobacter junii, i-Acinetobacter haemolyticus, i-Legionella pneumophila, i-Escherichia coli, i-Pseudomonas fluorescens, i-Candida albicans, i-Chlamydia pneumoniae, i-Respiratory adenovirus, i-Enterococcus, noma amasampula aqukethe ezinye izakhi zofuzo ezingamelana nemithi i-CTX, i-mecA, i-SME, i-SHV, i-TEM, njll. c) Okulwa nokuphazamiseka: I-Mucin, i-Minocycline, i-Gentamicin, i-Clindamycin, i-Imipenem, i-Cefoperazone, i-Meropenem, i-Ciprofloxacin Hydrochloride, i-Levofloxacin, i-Clavulanic acid, i-Roxithromycin zikhethiwe ukuze kuhlolwe ukuphazamiseka, futhi imiphumela ikhombisa ukuthi izinto eziphazamisayo ezishiwo ngenhla azinawo umthelela ophazamisayo ekutholakaleni kwezakhi zofuzo zokumelana ne-carbapenem i-KPC, i-NDM, i-OXA48, i-OXA23, i-VIM, kanye ne-IMP. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (I-FQD-96A，I-HangzhouUbuchwepheshe be-Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-reagent yokukhipha enconywayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Engeza u-200μL we-saline evamile ku-thallus precipitate. Izinyathelo ezilandelayo kufanele zilandele imiyalelo yokukhipha, futhi ivolumu yokususa enconywayo ingu-100μL.
Inketho 2.
I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co., Ltd. Ukukhipha kufanele kuqalwe ngokuhambisana nesinyathelo sesi-2 somyalelo wokusebenzisa (engeza u-200μL we-buffer GA ku-thallus precipitate, bese unyakazisa kuze kube yilapho i-thallus imile ngokuphelele). Sebenzisa amanzi angenayo i-RNase/DNase ukuze ukhiphe amanzi, futhi ivolumu yokukhipha amanzi enconyiwe ingu-100μL.
Inketho 3.
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Sample Release Release Reagent. Isampula ye-sputum idinga ukugezwa ngokungeza i-1mL ye-saline evamile ku-thallus precipitate eshiwo ngenhla, ifakwe ku-centrifuge ku-13000r/min imizuzu emi-5, bese i-supernatant ilahlwa (gcina i-10-20µL ye-supernatant). Ukuze uthole i-colony emsulwa kanye ne-rectal swab, engeza i-50μL ye-release release release release release release release release release release release release, bese izinyathelo ezilandelayo kufanele zikhishwe ngokwemiyalelo yokusebenzisa.
