Ikhithi Yokuthola I-Nucleic Acid (i-Fluorescence PCR)-RUO eyomisiwe ngeqhwa
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid (i-Fluorescence PCR)-RUO eyomisiwe ngeqhwa
I-Epidemiology
Umkhuhlane we-dengue (DF), obangelwa ukutheleleka kwe-denguevirus (DENV), ungenye yezifo ezithathelwanayo ze-arbovirus eziwubhubhane kakhulu. Indlela yawo yokudlulisela ihlanganisa i-Aedes aegypti kanye ne-Aedes albopictus. I-DF ivame kakhulu ezindaweni ezishisayo nezishisayo. I-DENV ingeyama-flavivirus ngaphansi kwe-flaviviridae, futhi ingahlukaniswa ngama-serotypes amane ngokuya nge-antigen engaphezulu. Izimpawu zemitholampilo zokutheleleka kwe-DENV zifaka phakathi ikhanda elibuhlungu, umkhuhlane, ubuthakathaka, ukwanda kwe-lymph node, i-leukopenia njll., kanye nokopha, ukushaqeka, ukulimala kwesibindi noma ngisho nokufa ezimweni ezimbi kakhulu. Eminyakeni yamuva nje, ushintsho lwesimo sezulu, ukusabalala kwamadolobha, intuthuko esheshayo yezokuvakasha kanye nezinye izici kuhlinzeke ngezimo ezisheshayo nezilula zokudlulisa nokusabalala kwe-DF, okuholela ekwandeni okuqhubekayo kwendawo yobhubhane lwe-DF. Ngakho-ke, ukusungulwa kwendlela yokuxilonga i-DENV elula, eqondile futhi esheshayo kubaluleke kakhulu ekuxilongweni kwe-DF emtholampilo.
Amapharamitha Obuchwepheshe
| Isitoreji | 2-8℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-serum |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | Amakhophi angu-500/mL |
| Ukucaciswa | Ukuhlolwa akunawo umphumela omuhle kumasampula e-serum angenayo igciwane le-dengue; imiphumela yokuhlolwa kokuphazamiseka ikhombisa ukuthi uma ukugcwala kwe-bilirubin ku-serum kungaphezu kuka-168.2μmol/mL, ukugcwala kwe-hemoglobin okukhiqizwa yi-hemolysis akungaphezu kuka-130g/L, ukugcwala kwamafutha egazini akungaphezu kuka-65mmol/mL, kanti ukugcwala okuphelele kwe-IgG ku-serum akungaphezu kuka-5mg/mL, akukho mphumela ekutholakaleni kwegciwane le-dengue; sebenzisa igciwane le-Hepatitis A, igciwane le-Hepatitis B, igciwane le-Hepatitis C, igciwane le-Herpes, igciwane le-Eastern equine encephalitis, igciwane le-Hantaan, igciwane le-Bunia, igciwane le-West Nile, igciwane le-Zika kanye namasampula e-serum ye-genomic yabantu ukuze kuhlolwe ukusabela okuxubile, futhi imiphumela ikhombisa ukuthi akukho ukusabela okuxubile phakathi kwale kit kanye namagciwane angenhla. |
| Izinsimbi Ezisebenzayo | Uhlobo I: Izinhlelo ze-Biosystems ezisetshenzisiwe 7500 Real-Time PCR, Izinhlelo ze-QuantStudio®5 Real-Time PCR, Izinhlelo ze-SLAN-96P Real-Time PCR (Hongshi Medical Technology Co., Ltd.), Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 Real-Time PCR. Uhlobo II: EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
|
Ukugeleza Komsebenzi
Ama-reagent adinga kodwa awanikezwa:
Uhlobo I: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
Uhlobo II: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3023-8), ingasetshenziswa ne-EudemonTM AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ngaphambi kokusebenzisa i-Type II test reagent, ikhasimende line-reagent yokukhipha esekelayo kanye nethuluzi lokukhulisa, futhi unjiniyela uzoqondisa ikhasimende ukuthi lenze umsebenzi wokuhlola lapho lisebenzisa okokuqala.
Izinto ezisetshenziswayo zidinga kodwa azinikezwa:Amathiphu Angenazo i-DNase/RNase, amagilavu alahlwayo, amashubhu e-EP angenazo i-DNase/RNase, i-centrifuge, isitendi sikamazibuthe, imichilo yamashubhu angu-8 ye-PCR.
