Ikhithi Yokuthola Ukuguquka Kwezakhi Zofuzo Ze-EGFR Yomuntu (i-PCR ye-Fluorescence)-RUO

Umdlavuza wamaphaphu ube yimbangela ehamba phambili yokufa komdlavuza emhlabeni jikelele, usongela kakhulu impilo yabantu. Umdlavuza wamaphaphu ongewona amangqamuzana amancane ubanga cishe u-80%-85% weziguli zomdlavuza wamaphaphu. I-EGFR njengamanje iyisisulu esibaluleke kakhulu sama-molecule ekwelapheni umdlavuza wamaphaphu ongewona amangqamuzana amancane. I-phosphorylation ye-EGFR ingakhuthaza ukukhula kwamaseli esimila, ukuhlukanisa, ukuhlasela, ukusabalala, ukulwa ne-apoptosis, futhi ikhuthaze i-angiogenesis yesimila. Ama-inhibitors e-tyrosine kinase e-EGFR (i-TKI) angavimba indlela yokubonisa ye-EGFR ngokuvimbela i-EGFR autophosphorylation, ngaleyo ndlela avimbele ukwanda nokwehlukaniswa kwamaseli esimila, akhuthaze i-apoptosis yamaseli esimila, anciphise i-angiogenesis yesimila, njll., ukuze kufezwe ukwelashwa okuqondiswe ku-tumor [1]. Inani elikhulu lezifundo likhombisile ukuthi ukusebenza kahle kokwelapha kwe-EGFR-TKI kuhlobene eduze nesimo sokuguqulwa kwezakhi zofuzo ze-EGFR, futhi kungavimbela ngqo ukukhula kwamaseli esimila ngokuguqulwa kwezakhi zofuzo ze-EGFR [2,3]. I-gene ye-EGFR itholakala engalweni emfushane ye-chromosome 7 (7p12), enobude obuphelele obungu-200Kb futhi iqukethe ama-exons angu-28. Indawo yokuguqulwa kwezakhi zofuzo itholakala kakhulu kuma-exons angu-18 kuya ku-21, ama-codons angu-746 kuya ku-753 deletion mutation ku-exon 19 angaba ngu-45% kanti i-L858R mutation ku-exon 21 iba ngu-40% kuya ku-45% [4]. Iziqondiso ze-NCCN Zokuxilongwa Nokwelashwa Komdlavuza Wamaphaphu Ongewona Amancane Amaseli zisho ngokusobala ukuthi ukuhlolwa kokuguqulwa kwezakhi zofuzo ze-EGFR kuyadingeka ngaphambi kokuphathwa kwe-EGFR-TKI. Le khithi yokuhlola isetshenziselwa ukuqondisa ukuphathwa kwemithi ye-epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), futhi ihlinzeka ngesisekelo semithi eyenzelwe wena iziguli ezinomdlavuza wamaphaphu ongewona amaseli amancane. Le khithi isetshenziselwa kuphela ukuthola izinguquko ezivamile ku-gene ye-EGFR ezigulini ezinomdlavuza wamaphaphu ongewona amaseli amancane. Imiphumela yokuhlolwa ingeyereferensi yomtholampilo kuphela futhi akufanele isetshenziswe njengesisekelo sodwa sokwelashwa kweziguli okuqondene nomuntu ngamunye. Odokotela kufanele bacabangele isimo sesiguli, izinkomba zemithi, kanye nokwelashwa. Ukusabela kanye nezinye izinkomba zokuhlolwa kwelabhorethri kanye nezinye izici kusetshenziswa ukwahlulela ngokuphelele imiphumela yokuhlolwa.