Uhlobo 1 lwegciwane le-Herpes Simplex
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid ye-HWTS-UR006 Herpes Simplex Virus Type 1 (i-Fluorescence PCR)
I-Epidemiology
Izifo ezithathelwana ngocansi (STD) ziseseyinye yezinsongo ezibalulekile ekuphepheni kwempilo yomphakathi emhlabeni wonke, okungaholela ekungazalini, ekubelethweni ngaphambi kwesikhathi, emathumba kanye nezinkinga eziningi ezinkulu.[3-6]. Kunezinhlobo eziningi zamagciwane e-STD, okuhlanganisa amagciwane, amagciwane, i-chlamydia, i-mycoplasma nama-spirochetes. Izinhlobo ezijwayelekile zihlanganisa i-neisseria gonorrhoeae, i-mycoplasma genitalium, i-chlamydia trachomatis, i-herpes simplex virus type 1, i-herpes simplex virus type 2, mycoplasma hominis, ureaplasma urealyticum, njll.
Isiteshi
| I-FAM | Uhlobo 1 lwegciwane le-Herpes Simplex (HSV1) |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomlomo wesibeletho yowesifazane,I-swab ye-urethra yabesilisa |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | 500Amakhophi/mL |
| Ukucaciswa | Hlola amanye amagciwane okutheleleka nge-STD, afana ne-treponema pallidum, i-chlamydia trachomatis, i-neisseria gonorrhoeae, i-mycoplasma hominis, i-mycoplasma genitalium, i-ureaplasma urealyticum, njll., akukho-cross-reactivity. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), ukukhishwa kufanele kwenziwe ngokwe-IFU ngokuqinile.
Inketho 2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhipha kufanele kwenziwe ngokwe-IFU, futhi ivolumu yokususa enconywayo ingu-80μL.
Inketho 3.
I-Nucleic Acid Extract noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuhambisana ne-IFU, futhi ivolumu yokususa enconywayo ingu-80μL.
Amasampula e-DNA akhishwe kufanele ahlolwe ngokushesha noma agcinwe ngaphansi kuka--18°C isikhathi esingaphezu kwezinyanga eziyi-7. Inani lokuqandisa nokuncibilikisa okuphindaphindiwe akufanele lidlule imijikelezo emi-4.
