I-CYP2C9 yomuntu kanye ne-VKORC1 Gene Polymorphism
Igama lomkhiqizo
I-HWTS-GE014A-Human CYP2C9 kanye ne-VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)
Isitifiketi
CE/TFDA
I-Epidemiology
I-Warfarin iyi-anticoagulant yomlomo evame ukusetshenziswa emisebenzini yomtholampilo njengamanje, ehloselwe kakhulu ukuvimbela nokwelashwa kwezifo ze-thromboembolic.Kodwa-ke, i-warfarin inefasitela lokwelapha elilinganiselwe futhi ihluka kakhulu phakathi kwezinhlanga ezahlukene kanye nabantu ngabanye.Izibalo zibonise ukuthi umehluko wethamo elizinzile kubantu abahlukene ungaba izikhathi ezingaphezu kwezingu-20.Ukopha okungathandeki kwenzeka ku-15.2% weziguli ezithatha i-warfarin unyaka ngamunye, lapho u-3.5% wazo ziba nokopha okubulalayo.Ucwaningo lwe-pharmacogenomic lubonise ukuthi i-polymorphism yofuzo ye-enzyme eqondiwe i-VKORC1 kanye ne-enzyme ye-metabolic CYP2C9 ye-warfarin iyisici esibalulekile esithinta umehluko kumthamo we-warfarin.I-Warfarin iyisivimbeli esithile se-vitamin K epoxide reductase (VKORC1), futhi ngaleyo ndlela ivimbela ukuhlanganiswa kwezinto ezijiyisayo ezihlanganisa uvithamini K futhi inikeza i-anticoagulation.Inani elikhulu locwaningo libonise ukuthi i-polymorphism yofuzo yomgqugquzeli we-VKORC1 iyisici esibaluleke kakhulu esithinta umjaho nokuhluka komuntu ngamunye kumthamo odingekayo we-warfarin.I-Warfarin yenziwa i-CYP2C9, futhi izinguquko zayo zehlisa kakhulu imetabolism ye-warfarin.Abantu abasebenzisa i-warfarin basengozini enkulu (ephindwe kabili ukuya kathathu) yokopha ekuqaleni kokusetshenziswa.
Isiteshi
| FAM | I-VKORC1 (-1639G>A) |
| CY5 | I-CYP2C9*3 |
| VIC/HEX | IC |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo lwesifanekiso | Igazi elisha le-EDTA elingenayo i-anticoagulated |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Ukucaciswa | Akukho ukuhlangana kabusha nokunye ukulandelana okungaguquki kakhulu kwe-genome yomuntu (ufuzo lwe-CYP2C19 yomuntu, isakhi sofuzo se-RPN2 yomuntu);ukuguqulwa kwe-CYP2C9*13 kanye ne-VKORC1 (3730G>A) ngaphandle kwebanga lokutholwa kwale khithi |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).
