I-Cuman Cument2C9 ne-VKORC1 gene polymorphism
Igama lomkhiqizo
I-HWTS-GE014A-Human Cy2C9 ne-VKORC1 Gene Polymorphism Detection Dection Kit (Fluorescence PCR)
Isitifiketi
Ce / tfda
I-Epidemiology
I-Warfarin iyi-anticoagulan yomlomo esetshenziswa kakhulu ekusebenzeni emtholampilo Okwamanje, okuhloselwe ikakhulukazi ukuvikela nokwelashwa kwezifo ze-thromboemolic. Kodwa-ke, iWarfarin inefasitela lokwelashwa elilinganiselwe futhi liyahlukahluka kakhulu phakathi kwezinhlanga ezahlukene kanye nabantu. Izibalo ziveze ukuthi umehluko womthamo ozinzile kubantu abahlukahlukene ungaba ngaphezu kwezikhathi ezingama-20. Ukuphuma okuxakile ukuphendula kwenzeka ngo-15.2% weziguli ezithatha i-warfarin unyaka ngamunye, lapho kuthi ama-3.5% akhulisa ukopha okubulalayo. Izifundo ze-pharmacogenomic zikhombisile ukuthi i-polymorphism ye-genetic ye-enzyme vkorc1 kanye ne-metabolic enzyme cyp2c9 yeWarfarin iyinto ebalulekile ethinta umehluko wethamo le-warfarin. I-Warfarin iyinhlangano ethile ye-Vitamin K epoxide Recuctase (VKORC1), futhi ngaleyo ndlela ivimbela ukuguquguquka kwesici okuvinjelwe okubandakanya i-Vitamin K futhi inikeze i-anticoagulation. Inani elikhulu lezifundo liveze ukuthi i-gene polymorphism ye-VKORC1 Promoter iyinto ebaluleke kakhulu ethinta umjaho kanye nokwehluka komuntu ngamunye emthanjeni odingekayo we-warfarin. I-Warfarin ihlanganiswe yi-CCP2C9, kanye nokuguqulwa kwayo kuhamba kancane kwe-metabolism ye-warfarin. Abantu abasebenzisa iWarfarin banobungozi obuphakeme (kabili kuya kathathu okuphezulu) kokopha esonweni lokusetshenziswa ekuqaleni.
Insungubezi
| -Yenza | I-VKORC1 (-1639G> A) |
| Cy5 | I-CYP2C9 * 3 |
| VIC / Hex | IC |
Amapharamitha Ezobuchwepheshe
| Ukubeka | Uketshezi: ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Igazi elisha le-anticoagulated |
| CV | ≤5.0% |
| Indlu edayisa insimbi | I-1.0NG / μL |
| Ukucacisa | Akukho okuphambana nokuphindaphindeka ngokulandelana okungaguquki kwe-genome yabantu (i-CUMUNT CYPS2C19 gene, gene ye-RPN2 yomuntu); Ukuguqulwa kwe-CCP2C9 * 13 ne-VKORC1 (3730g> a) ngaphandle kwebanga lokutholwa kwale kit |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-realent enconyiwe I-realent: I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3001, HWTS-3004-32, HWS-3004-48, HWS-3004-9) kanye ne-Macro & Micro-Test 3006).
