I-Human CYP2C9 kanye ne-VKORC1 Gene Polymorphism
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-GE014A-I-CYP2C9 Yomuntu kanye ne-VKORC1 Gene Polymorphism (i-Fluorescence PCR)
Isitifiketi
I-CE/TFDA
I-Epidemiology
I-Warfarin iyi-anticoagulant yomlomo evame ukusetshenziswa emitholampilo njengamanje, ehloselwe kakhulu ukuvimbela nokwelapha izifo ze-thromboembolic. Kodwa-ke, i-warfarin inefasitela lokwelashwa elilinganiselwe futhi iyahlukahluka kakhulu phakathi kwezinhlanga ezahlukene kanye nabantu ngabanye. Izibalo zikhombisile ukuthi umehluko womthamo ozinzile kubantu abahlukene ungaba ngaphezu kwezikhathi ezingama-20. Ukopha okungekuhle kwenzeka ku-15.2% weziguli ezithatha i-warfarin minyaka yonke, lapho u-3.5% wazo eba nokopha okubulalayo. Izifundo ze-Pharmacogenomic zikhombisile ukuthi i-polymorphism yezakhi zofuzo ye-enzyme eqondiwe i-VKORC1 kanye ne-enzyme ye-metabolic CYP2C9 ye-warfarin iyisici esibalulekile esithinta umehluko womthamo we-warfarin. I-Warfarin iyisithiyo esithile se-vitamin K epoxide reductase (VKORC1), futhi ngaleyo ndlela ivimbela ukwakheka kwe-clotting factor okubandakanya i-vitamin K futhi ihlinzeka ngokuvimbela ukujiya kwegazi. Inani elikhulu lezifundo likhombisile ukuthi i-polymorphism yezakhi zofuzo ye-VKORC1 promoter iyisici esibaluleke kakhulu esithinta umehluko wohlanga kanye nomuntu ngamunye kumthamo odingekayo we-warfarin. I-Warfarin iguqulwa yi-CYP2C9, futhi izakhi zayo eziguquliwe zinciphisa kakhulu ukusebenza kwe-warfarin. Abantu abasebenzisa i-warfarin banengozi ephezulu (ephindwe kabili kuya kathathu) yokopha ekuqaleni kokusetshenziswa.
Isiteshi
| I-FAM | I-VKORC1 (-1639G>A) |
| CY5 | I-CYP2C9*3 |
| I-VIC/HEX | IC |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Igazi elisha elingasebenzi kahle le-EDTA |
| CV | ≤5.0% |
| I-LoD | 1.0ng/μL |
| Ukucaciswa | Akukho ukuhlangana kwezakhi zofuzo nezinye izinhla zofuzo ezilandelanayo kakhulu (i-human CYP2C19 gene, i-human RPN2 gene); ukuguqulwa kwe-CYP2C9*13 kanye ne-VKORC1 (3730G>A) ngaphandle kobubanzi bokutholwa kwale khithi. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-reagent yokukhipha enconywayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
