I-Methylated Yomuntu NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene
Igama lomkhiqizo
I-HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Detection Kit (Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
Kubantu abadala, amaseli e-epithelial amathumbu angaphezu kuka-10 8 awela odongeni lwamathumbu nsuku zonke, futhi akhishwa ngendle ngokusebenzisa i-peristalsis yamathumbu amakhulu.Ngenxa yamangqamuzana e-tumor maningi amathuba okuthi awele emgudwini wamathumbu wokwanda okungavamile, isihlalo seziguli zesimila samathumbu siqukethe amaseli amaningi anesifo kanye nezingxenye zamangqamuzana ezingavamile, okuyisisekelo esibonakalayo sokutholwa kwendle ezinzile.Ucwaningo luthole ukuthi ukuguqulwa kwe-methylation yabagqugquzeli bezakhi zofuzo kuyisenzakalo sakuqala ku-tumorigenesis, futhi izinto zofuzo ezitholakala kumasampula esitulo eziguli ezinomdlavuza we-colorectal zingabonisa ukuba khona komdlavuza emathunjini ngaphambili.
I-NDRG4, eyaziwa nangokuthi i-SMAP-8 ne-BDM1, ingenye yamalungu amane omndeni we-NDRG gene (NDRG1-4), okuye kwaboniswa ukuthi kuhlotshaniswa nokwanda kwamaseli, ukuhlukaniswa, ukuthuthukiswa kanye nokucindezeleka.Kuqinisekisiwe ukuthi i-NDRG4 methylation iwuphawu lwezinto eziphilayo olungase lube khona ukuze kutholwe umdlavuza we-colorectal kumasampula esitulo.
U-SEPT9 uyilungu lomndeni we-Septin gene, equkethe okungenani izakhi zofuzo eziyi-13 ezifaka ikhodi yesizinda se-GTPase esilondoloziwe esingabopha amaprotheni ahlobene ne-cytoskeleton, futhi ehlotshaniswa nokuhlukaniswa kwamaseli kanye ne-tumorigenesis.Ucwaningo luthole ukuthi okuqukethwe kofuzo lwe-methylated Septin9 kunyuswa ngokuphawulekayo kumasampula esitulo ezigulini ezinomdlavuza we-colorectal.
Amaprotheni ayimfihlo ahlobene ne-frizzled (sFRPs) angamaprotheni ancibilikayo ayisigaba sabaphikisi bendlela ye-Wnt ngenxa ye-homology yabo yesakhiwo ephezulu kuma-frizzled (Fz) receptor for Wnt signing.Ukungasebenzi kofuzo lwe-SFRP kuphumela ekusebenzeni okungalawulwa kokusayina kwe-Wnt okuhlobene nomdlavuza we-colorectal.Njengamanje, i-SFRP2 methylation esitokisini ingasetshenziswa njenge-biomarker engahlaseli ekuxilongeni umdlavuza we-colorectal.
I-BMP3 iyilungu lomndeni omkhulu we-TGF-B futhi ngaleyo ndlela idlala indima ebalulekile ekukhuleni kombungu ngokunxenxa kanye nokubumba ukwakheka kwamathambo okuqala.I-BMP3 i-hypermethylated kumdlavuza we-colorectal futhi ingasetshenziswa njengomaka obalulekile wesimila.
I-SDC2 iyindawo yeseli ye-heparan sulfate proteoglycan ebandakanyeka ekulawuleni izinqubo eziningi ze-physiological and pathological.Ukucutshungulwa ngokomzimba kuhlanganisa ukwanda kwamaseli, ukuhlukaniswa, ukunamathela, inhlangano ye-cytoskeletal, ukufuduka, ukuphulukiswa kwesilonda, ukuxhumana kwe-cell-matrix, i-angiogenesis;Izinqubo ze-Pathological zihlanganisa ukuvuvukala nomdlavuza.Izinga le-methylation lofuzo lwe-SDC2 ezicutshini zomdlavuza we-colorectal laliphezulu kakhulu kunalelo elitholakala ezicutshini ezivamile.
Isiteshi
| Isilondolozi sokusabela A | VIC/HEX | i-methylated NDRG4 gene |
| I-ROX | i-methylated SEPT9 gene | |
| CY5 | ukulawula kwangaphakathi | |
| Isilondolozi sokusabela B | VIC/HEX | i-methylated SFRP2 yofuzo |
| I-ROX | i-methylated BMP3 gene | |
| FAM | i-methylated SDC2 gene | |
| CY5 | ukulawula kwangaphakathi |
Ukuhunyushwa
| Gene | Isiteshi Sesiginali | Ct Inani | Ukuhunyushwa |
| I-NDRG4 | I-VIC (HEX) | Inani le-CT≤38 | I-NDRG4 enhle |
| Ct value>38 noma ngaphansi | I-NDRG4 negative | ||
| SEPT9 | I-ROX | Inani le-CT≤38 | U-SEPT9 omuhle |
| Ct value>38 noma ngaphansi | SEPT9 negative | ||
| I-SFRP2 | I-VIC (HEX) | Inani le-CT≤38 | I-SFRP2 enhle |
| Ct value>38 noma ngaphansi | I-SFRP2 inegethivu |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo lwesifanekiso | Isampula yendle |
| CV | ≤5.0% |
| Ukucaciswa | Akukho ukusabela okuphambene nomdlavuza wesibindi, umdlavuza we-bile duct, umdlavuza wegilo kanye nomdlavuza wamaphaphu |
| Izinsimbi Ezisebenzayo | I-QuantStudio ®5 I-Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).
