Umkhuhlane A/B
Igama lomkhiqizo
I-HWTS-RT003A Influenza A/B Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Igciwane loMkhuhlane A yisifo esithathelwanayo esinamandla sokuphefumula, esinezinhlobo eziningi ezincane njenge-H1N1 kanye ne-H3N2, ezijwayele ukuguquka futhi zaqubuka emhlabeni wonke.Ukushintsha kwe-Antigeniki kubhekisela ekuguqulweni kwegciwane lomkhuhlane A, okuholela ekuveleni kohlobo oluncane olusha.Amagciwane oMkhuhlane B ahlukaniswe izigaba ezimbili ezinkulu, i-Yamagata ne-Victoria.Amagciwane oMkhuhlane B ane-antigenic drift kuphela, futhi agwema ukubhekwa kanye nokucaciswa kwamasosha omzimba womuntu ngokuguqulwa kwawo.Kodwa-ke, izinga lokuguquguquka kwegciwane lomkhuhlane B lihamba kancane kunegciwane lomkhuhlane A, futhi igciwane lomkhuhlane B lingabangela ukutheleleka komgudu wokuphefumula womuntu futhi kubangele izifo eziwumshayabhuqe.
Isiteshi
| FAM | IFV A |
| I-ROX | Ukulawula Kwangaphakathi |
| VIC/HEX | IFV B |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | i-oropharyngeal swab |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | I-IFV A:500Copies/mL,IFV B:500Copies/mL |
| Ukucaciswa | 1. I-Cross-reactivity: akukho ukuphinda kusebenze phakathi kwale khithi kanye nohlobo lwe-adenovirus 3, 7, i-coronavirus yabantu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, ne-HCoV-NL63, i-cytomegalovirus, enterovirus, parainfluenza virus, virus measles, human metapneumovirus, mumps virus, zokuphefumula syncytial virus uhlobo B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacillus, moraraxella catarrhalismycomycosis, tidis , i-neisseria gonorrhoeae, i-pseudomonas aeruginosa, i-staphylococcus aureus, i-staphylococcus epidermidis, i-streptococcus pneumoniae, i-streptococcus pyogenes, i-streptococcus salivarius kanye ne-DNA ye-genomic yomuntu. 2. Ukuhlolwa kokuphazanyiswa: Izinto eziphazamisayo zikhethwa njenge-mucin (60mg/mL), igazi lomuntu, i-oxymetazoline (2mg/mL), isulfure (10%), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide ( 20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL ), azithromycin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir phosphate (60ng/mL), ribavirin (10mg/L), kanye nemiphumela ibonisa ukuthi izinto eziphazamisayo ngokugxila ngenhla azinakho ukusabela okuphazamisayo ekutholweni kwekhithi. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System kanye ne-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Ama-reagents okukhipha anconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuvumelana ne-IFU.Ivolumu yesampula yokukhipha ngu200μL.Ivolumu ye-elution enconyiwe ingu-80μL.
Inketho yesi-2.
Ama-reagents okukhipha anconyiwe: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ukukhishwa kufanele kwenziwe ngokwe-IFU.
Inketho yesi-3.
Ama-reagents okukhipha anconyiwe: I-Nucleic Acid Extraction noma Ikhithi Yokuhlanza (YDP315-R).Ukukhishwa kufanele kwenziwe ngokwe-IFU.Ivolumu yesampula yokukhipha ngu-140μL.Ivolumu ye-elution enconyiwe ngu-60μL.
