I-Mycobacterium Tuberculosis INH Ukumelana

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola ngokufanelekile ukuguqulwa kofuzo kwe-amino acid engama-315 yofuzo lwe-katG (K315G>C) kanye nokuguqulwa kofuzo kwesifunda somgqugquzeli wofuzo lwe-InhA (- 15 C>T).


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama Lomkhiqizo

HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)

Isitifiketi

I-FDA yaseMyanmar

I-Epidemiology

I-Isoniazid, umuthi obalulekile wokulwa nesifo sofuba owethulwa ngo-1952, ungomunye wemishanguzo esebenza kahle kakhulu yokwelapha okuhlangene kwesifo sofuba esisebenzayo kanye nomuthi owodwa wesifo sofuba esicashile.

I-KatG iyisakhi sofuzo esiyinhloko esifaka ikhodi ye-catalase-peroxidase futhi ukuguqulwa kofuzo kwe-katG kungakhuthaza ukwakheka kodonga lwamaseli e-mycolic acid, kwenze amagciwane amelane ne-isoniazid.Inkulumo ye-KatG ihlotshaniswa kabi nezinguquko ku-INH-MIC, futhi ukwehla okuphindwe kabili kwesisho se-katG kuphumela ekukhuphukeni okukhudlwana okuphindwe ka-2 ku-MIC.Enye imbangela yokumelana ne-isoniazid ku-mycobacterium tuberculosis kwenzeka lapho ukufakwa kwesisekelo, ukususwa noma ukuguqulwa kwenzeka endaweni yofuzo ye-InhA ye-mycobacterium tuberculosis.

Isiteshi

I-ROX indawo ye-inhA (-15C>T)·
CY5

isayithi le-katG (315G>C).

I-VIC (HEX)

IS6110

Imingcele Yezobuchwepheshe

Isitoreji ≤-18℃ ebumnyameni
Impilo yeshelufu

Izinyanga ezingu-12

Uhlobo lwesifanekiso

Isikhwehlela

CV ≤5.0%
LoD

1 × 103amagciwane/mL

Ukucaciswa Ukusebenza kabusha okungenasiphambano nokuguqulwa kwezindawo ezine ezimelana nezidakamizwa (511, 516, 526 kanye 531) zofuzo lwe-rpoB ngaphandle kwebanga lokutholwa lekhithi yokuthola.

Izinsimbi Ezisebenzayo:

I-Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

I-QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

I-LineGene 9600 Plus Real-Time PCR Detection System

I-MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Real-Time PCR System

I-BioRad CFX Opus 96 Real-Time PCR System

Ukugeleza komsebenzi

4697e0586927f02cf6939f68fc30ffc


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