I-Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF)，Ukumelana(INH)

I-Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF)，Resistance(INH) Isithombe Esifakiwe

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwekhwalithi ye-in vitro ye-Mycobacterium tuberculosis DNA esikhwehleleni somuntu, isiko eliqinile (LJ Medium) kanye nesiko le-liquid (MGIT Medium), uketshezi lwe-bronchial lavage, kanye nokuguqulwa kwe-507-533 amino acid codon region (81bp, i-rifampicin ukumelana ne-rifampicin enquma i-rifampicin isifunda sofuzo lwe-rifampiumcin, isifunda se-rifampicin esinquma i-rifampicin i-tuberbacteriumcin) kanye noguquko ezindaweni eziyinhloko zokuguqulwa kwe-Mycobacterium tuberculosis isoniazid resistance.Ihlinzeka ngosizo ekuhlonzweni kwesifo sofuba i-Mycobacterium, futhi ithola izakhi zofuzo eziyinhloko zokumelana ne-rifampicin ne-isoniazid, esiza ukuqonda ukumelana nomuthi we-Mycobacterium tuberculosis etheleleke isiguli.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), (INH) Detection Kit (Melting Curve)

I-Epidemiology

I-Mycobacterium tuberculosis, maduze nje njenge-Tubercle bacillus (TB), igciwane le-pathogenic elidala isifo sofuba, futhi okwamanje, imithi elwa nesifo sofuba esetshenziswa kakhulu yomugqa wokuqala ihlanganisa i-isoniazid, i-rifampicin ne-ethambutol, njll.^[1]. Kodwa-ke, ngenxa yokusetshenziswa okungalungile kwezidakamizwa ezilwa nesifo sofuba kanye nezici zesakhiwo sodonga lwamaseli we-mycobacterium tuberculosis uqobo, isifo sofuba se-mycobacterium sesisungule ukumelana nemithi elwa nesifo sofuba, futhi uhlobo oluyingozi kakhulu isifo sofuba esingazweli ezidakamizwa (MDR-TB), esingazweli emishanguzweni emibili evamile futhi ephumelelayo, i-rifampicin kanye ne-rifampicin.^[2].

Inkinga yokumelana nemithi yofuba ikhona kuwo wonke amazwe ahlolwe yi-WHO. Ukuze kuhlinzekwe izinhlelo zokwelashwa ezinembayo ezigulini ezinesifo sofuba, kuyadingeka ukuthi kutholwe ukumelana nemithi elwa nesifo sofuba, ikakhulukazi ukumelana ne-rifampicin, osekuyisinyathelo sokuxilonga esituswe yi-WHO ekwelapheni isifo sofuba.^[3]. Nakuba ukutholakala kokumelana ne-rifampicin kucishe kulingane nokutholakala kwe-MDR-TB, ukuthola kuphela ukumelana ne-rifampicin kuziba iziguli ezine-INH engamelana ne-mono (ibhekisela ukumelana ne-isoniazid kodwa ezwela ku-rifampicin) kanye ne-mono-resistant rifampicin (ukuzwela ku-isoniazid kodwa ukumelana ne-rifampicin), okungase kuholele ezigulini eziphathwe ngendlela engaqondakali ekuqaleni. Ngakho-ke, ukuhlolwa kokumelana ne-isoniazid kanye ne-rifampicin kuyizidingo ezidingekayo okungenani kuzo zonke izinhlelo zokulawula i-DR-TB^[⁴^].

Imingcele Yezobuchwepheshe

Isitoreji	≤-18℃
Impilo yeshelufu	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	Isampula yesikhwehlela, Isiko Eliqinile (LJ Medium), Isiko Loketshezi (MGIT Medium)
CV	<5.0%
LoD	I-LoD yekhithi yokuthola isifo sofuba i-Mycobacterium ngamabhaktheriya ayi-10/mL;i-LoD yekhithi yokuthola uhlobo lwasendle lwe-rifampicin kanye nohlobo oluguquguqukayo amabhaktheriya angu-150/mL; i-LoD yekhithi yokuthola uhlobo lwasendle lwe-isoniazid kanye nohlobo oluguquguqukayo ngamabhaktheriya angama-200/mL.
Ukucaciswa	I-1) Akukho ukusabela okuphambene lapho usebenzisa ikhithi ukuthola i-DNA yomuntu ye-genomic (500ng), ezinye izinhlobo ezingu-28 zezifo zokuphefumula, kanye nezinhlobo ezingu-29 ze-mycobacteria engeyona i-tuberculous (njengoba kukhonjisiwe kuThebula 3).2) Akukho ukusabela okuphambene lapho usebenzisa ikhithi ukuthola izindawo eziguqukayo zezinye izakhi zofuzo ezingazweli emuthini ze-rifampicin kanye ne-isoniazid ebucayi ye-Mycobacterium tuberculosis (njengoba kukhonjisiwe kuThebula 4).3) Izinto eziphazamisayo ezivamile kumasampuli azohlolwa, njenge-rifampicin (9mg/L), isoniazid (12mg/L), i-ethambutol (8mg/L), i-amoxicillin (11mg/L), i-oxymetazoline (1mg/L), i-mupirocin (20mg/L), i-pyrazinamide (45mg/L) (20mg/L) izidakamizwa, azinawo umthelela emiphumeleni yokuhlolwa kwekhithi.
Izinsimbi Ezisebenzayo	I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), I-BioRad CFX96 Real-Time PCR System