I-Mycobacterium Tuberculosis Nucleic Acid kanye ne-Rifampicin(RIF)，Resistance(INH)

Isithombe Esifakiwe Se-Mycobacterium Tuberculosis Nucleic Acid kanye ne-Rifampicin(RIF)，Resistance(INH)

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola i-Mycobacterium tuberculosis DNA ngaphakathi kwesibeletho, isiko eliqinile (i-LJ Medium) kanye nesiko loketshezi (i-MGIT Medium), uketshezi lokuhlanza i-bronchial, kanye nokuguqulwa kwezakhi zofuzo esifundeni se-codon esingu-507-533 amino acid (isifunda esingu-81bp, esinquma ukumelana ne-rifampicin) sejini le-rpoB lokumelana ne-Mycobacterium tuberculosis rifampicin, kanye nokuguqulwa kwezakhi zofuzo ezindaweni eziyinhloko zokuguqulwa kwezakhi zofuzo zokumelana ne-isoniazid ye-Mycobacterium tuberculosis. Inikeza usizo ekuxilongweni kokutheleleka kwe-Mycobacterium tuberculosis, futhi ithola izakhi zofuzo eziyinhloko zokumelana ne-rifampicin ne-isoniazid, okusiza ukuqonda ukumelana nemithi kwe-Mycobacterium tuberculosis etheleleke yisiguli.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-RT147 I-Mycobacterium Tuberculosis Nucleic Acid kanye ne-Rifampicin(RIF), (INH) Detection Kit (Melting Curve)

I-Epidemiology

I-Mycobacterium tuberculosis, efushaniswa njenge-Tubercle bacillus (TB), iyigciwane elibangela isifo sofuba, futhi njengamanje, imithi yokuqala yokulwa nesifo sofuba esetshenziswa kakhulu ihlanganisa i-isoniazid, i-rifampicin kanye ne-ethambutol, njll.^[1]Kodwa-ke, ngenxa yokusetshenziswa okungalungile kwemithi yokulwa nesifo sofuba kanye nezici zesakhiwo sodonga lweseli sesifo sofuba se-mycobacterium uqobo, isifo sofuba se-mycobacterium sesithuthukise ukumelana nemithi yemithi yokulwa nesifo sofuba, futhi uhlobo oluyingozi kakhulu yisifo sofuba esingamelana nemithi eminingi (i-MDR-TB), esimelana nemithi emibili evame kakhulu futhi esebenza kahle, i-rifampicin ne-isoniazid.^[2].

Inkinga yokumelana nemithi yesifo sofuba ikhona kuwo wonke amazwe ahlolwe yi-WHO. Ukuze kuhlinzekwe izinhlelo zokwelapha ezinembile kakhulu zeziguli zesifo sofuba, kubalulekile ukuthola ukumelana nemithi yesifo sofuba, ikakhulukazi ukumelana ne-rifampicin, okuye kwaba yisinyathelo sokuxilonga esinconywe yi-WHO ekwelapheni isifo sofuba.^[3]Nakuba ukutholakala kokumelana ne-rifampicin kucishe kufane nokutholakala kwe-MDR-TB, ukuthola kuphela ukumelana ne-rifampicin akunaki iziguli ezine-INH engamelani ne-mono (kubhekiselwa ekumelaneni ne-isoniazid kodwa ezwela ku-rifampicin) kanye ne-rifampicin engamelana ne-mono (ukuzwela ku-isoniazid kodwa ezwela ku-rifampicin), okungaholela ekutheni iziguli zibhekane nezindlela zokwelashwa zokuqala ezingafanele. Ngakho-ke, ukuhlolwa kokumelana ne-isoniazid kanye ne-rifampicin kuyizidingo ezincane ezidingekayo kuzo zonke izinhlelo zokulawula i-DR-TB.^[⁴^].

Amapharamitha Obuchwepheshe

Isitoreji	≤-18℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	Isampula Yesikhwehlela, Isiko Eliqinile (LJ Medium), Isiko Elimanzi (MGIT Medium)
CV	<5.0%
I-LoD	I-LoD yekhithi yokuthola isifo sofuba se-Mycobacterium ingu-10 bacteria/mL;I-LoD yekhithi yokuthola uhlobo lwe-rifampicin lwasendle kanye nohlobo lwe-mutant yi-bacteria engu-150/mL; I-LoD yekhithi yokuthola uhlobo lwasendle lwe-isoniazid kanye nohlobo lwe-mutant ingama-bacteria angu-200/mL.
Ukucaciswa	1) Akukho ukusabela okuhlangene lapho usebenzisa ikhithi ukuthola i-DNA ye-genomic yomuntu (500ng), ezinye izinhlobo ezingama-28 zezifo zokuphefumula, kanye nezinhlobo ezingama-29 ze-mycobacteria engeyona i-tuberculous (njengoba kuboniswe kuThebula 3).2) Akukho ukusabela okuhlangene lapho usebenzisa ikhithi ukuthola izindawo zokuguqulwa kwezakhi zofuzo ze-rifampicin kanye ne-Mycobacterium tuberculosis ezwela i-isoniazid (njengoba kuboniswe kuThebula 4).3) Izinto ezivame ukuphazamisa amasampula okufanele ahlolwe, njenge-rifampicin (9mg/L), i-isoniazid (12mg/L), i-ethambutol (8mg/L), i-amoxicillin (11mg/L), i-oxymetazoline (1mg/L), i-mupirocin (20mg/L), i-pyrazinamide (45mg/L), i-zanamivir (0.5mg/L), imithi ye-dexamethasone (20mg/L), ayinawo umthelela emiphumeleni yokuhlolwa kwekhithi.
Izinsimbi Ezisebenzayo	Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Uhlelo lwe-BioRad CFX96 Real-Time PCR