I-Mycobacterium Tuberculosis Nucleic Acid ne-Rifampicin (RIF), ukumelana (INH)

I-Mycobacterium Tuberculosis nuberculosis nucleic acid ne-rifampicin (RIF), ukumelana (INH) isithombe esifakiwe

Incazelo emfushane:

Le khithi isetshenziselwa ukutholwa kwe-vitro efanelekile ye-Mycobacterium Tuberculosis Duberculosis DNA Sputmum, isiko eliqinile (i-MGIT medium) kanye ne-bronchial fluid, kanye nokuguqulwa kwe-audium amino acid coco (81bp , Ukumelana nokumelana kwe-Rifampicin Ukunquma Isifunda) se-RPOB gene ye-Mycobacterium Ukumelana ne-Tuberculosis Rifampicin, kanye nokuguqulwa kwezindawo zokuguqulwa kwe-Mycobacterium Tuberculosis Isoniazid Ukumelana ne-foniazid. ye-mycobacterium tuberculosis etheleleke isiguli.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-RT147 MyCobacterium Tuberculosis Nucleic Acid ne-Rifampicin (RIF), (INH) Kit Detection (Chingting Curve)

I-Epidemiology

Isifo sofuba se-Mycobacterium, kungekudala njenge-tubercle bactellus (TB), i-bacterium ye-pathogenic ebangela isifo sofuba, futhi njengamanje, izidakamizwa ezijwayelekile zokulwa nesifo sofuba zifaka i-isoniazid, i-rifampicin kanye ne-ethambutol, njll.^[1]. Kodwa-ke, ngenxa yokusetshenziswa okungalungile kwezidakamizwa zokulwa nesifo sofuba kanye nezimpawu zokwakheka kwesifo seseli se-Mycobacterium Tuberculosis uqobo, ifomu eliyingozi kakhulu liyisifo sofuba esivimbanayo (i-MDR- I-TB), emelana nezidakamizwa ezimbili ezivame kakhulu futhi ezisebenzayo, iRifampicin ne-isoniazid^[2].

Inkinga yokuphikiswa kwezifo zesifo sofuba ikhona kuwo wonke amazwe ahlolwe ngubani. Ukuze unikeze amasu anembe kakhudlwana wezokwelapha iziguli zesifo sofuba, kuyadingeka ukuthola ukumelana nezidakamizwa zokulwa nesifo sofuba, ikakhulukazi ukumelana kwe-rifampicin, okuye kwaba yisinyathelo sokuxilonga esinconywe yilowo ophathweni kwesifo sofuba^[3]. Yize ukutholwa kokuphikiswa kwe-rifampicin kucishe kulingane nokutholwa kwe-MDR-TB, ukuthola ukumelana kwe-Rifampicin kunganaki iziguli ezine-iSoniazid (okubhekisele ku-Rifampicin) kanye ne-mononiazid (ukuzwela ku-isoniazid (ukuzwela ku-isoniazid kepha ukumelana nakho I-Rifampicin), engaholela ezigulini ezifakwe ngaphansi kokwelashwa okungekho emthethweni ama-regimens. Ngakho-ke, ukuhlolwa kokumelana ne-isoniazid kanye ne-rifampicin kunezidingo ezidingekayo kuzo zonke izinhlelo zokulawula i-DR-TB^[⁴^].

Amapharamitha Ezobuchwepheshe

Ukubeka	≤-18 ℃
Ishalofu-impilo	Izinyanga eziyi-12
Uhlobo lwe-Specimen	Isampula ye-Sputum, isiko eliqinile (nj humenti), isiko le-liquid (MGIT Medium)
CV	<5.0%
Indlu edayisa insimbi	I-Lod yekhithi yokuthola i-MyCobacterium Tuberculosis ingama-bacterium ayi-10 / ml;I-Lod yekhithi yokuthola uhlobo lwe-rifampicin lwasendle nohlobo oluguqukayo lungama-bacterio ayi-150 / ml; I-Lod yekhithi yokuthola i-isoniazid yohlobo lwasendle nohlobo lwama-bacterium angama-200 / ml.
Ukucacisa	1) Akukho ukusabela kwesiphambano lapho usebenzisa ikhithi ukuthola i-DNA yabantu (500ng), ezinye izinhlobo ezingama-28 zamagciwane okuphefumula, nezinhlobo ezingama-29 ze-Mycobacteria (njengoba kukhonjisiwe kuThebula 3).2) Akukho ukusabela kwesiphambano lapho usebenzisa i-kit ukuthola amasayithi wokuguqula ezinye izinhlobo zofuzo ezingamelana nezidakamizwa ne-isoniazid ebucayi i-mycobacterium isifo sofuba (njengoba kukhonjisiwe kuThebula 4).3) Izinto ezivamile zokuphazanyiswa kumasampula ukuze zihlolwe, njengeRifampicin (9mg / L), i-Isoniazid (12mg / L), i-Amoxicillin (i-11mg / l), i-OxyMeToline (20mg / L), i-Pyrazinamide (45mg / L), Zanamivir (0.5mg / L), Dexamethasone (20mg / L) izidakamizwa, awunamthelela emiphumeleni yokuhlolwa kwekhithi.
Izinsimbi ezisebenzayo	SLAN-96P real-time PCRS Systems (Hongshi Medical Technology Co, Ltd.), I-Biorad CFX96 Uhlelo lwe-Real-Time PCR