I-Chlamydia Trachomatis Nucleic Acid
Igama Lomkhiqizo
I-HWTS-UR001A-Chlamydia Trachomatis Nucleic Acid Detection Kit (Fluorescence PCR)
Ukusetshenziswa Okuhlosiwe
Le khithi isetshenziselwa ukutholwa kwekhwalithi ye-Chlamydia trachomatis nucleic acid emchameni wesilisa, i-swab ye-urethral yabesilisa, namasampula womlomo wesibeletho wesifazane.
I-Epidemiology
I-Chlamydia trachomatis (CT) iwuhlobo lwe-prokaryotic microorganism ene-parasitic eqinile kumaseli e-eukaryotic.I-Chlamydia trachomatis ihlukaniswe ngama-serotypes e-AK ngokuya ngendlela ye-serotype.Izifo ze-Urogenital tract zibangelwa kakhulu yi-trachoma biological variant DK serotypes, futhi abesilisa babonakaliswa kakhulu njenge-urethritis, engakhululeka ngaphandle kokwelashwa, kodwa iningi labo liba elingapheli, ngezikhathi ezithile lithuthuke, futhi lingahlanganiswa ne-epididymitis, i-proctitis, njll. kungabangelwa urethritis, cervicitis, njll, kanye nezinkinga ezimbi kakhulu ze-salpingitis.
I-Epidemiology
FAM: Chlamydia trachomatis (CT)·
I-VIC(HEX): Ukulawula Kwangaphakathi
Izilungiselelo ze-PCR Amplification Conditions
Isinyathelo | Imijikelezo | Izinga lokushisa | Isikhathi | Qoqa ama-Fluorescent Signals noma Cha |
1 | 1 umjikelezo | 50℃ | 5 imiz | No |
2 | 1 umjikelezo | 95℃ | 10 imiz | No |
3 | 40 imijikelezo | 95℃ | 15 amasekhondi | No |
4 | 58℃ | 31 isekhondi | Yebo |
Imingcele Yezobuchwepheshe
Isitoreji | ≤-18℃ ebumnyameni |
Impilo yeshelufu | Izinyanga ezingu-12 |
Uhlobo lwesifanekiso | Ukuphuma komchamo kwabesilisa, Ukuphuma komlomo wesibeletho kwabesifazane, Umchamo Wendoda |
Ct | ≤38 |
CV | <5.0% |
LoD | 400Amakhophi/mL |
Ukucaciswa | Akukho ukuphinda kusebenze ukuze kutholwe amanye amagciwane atheleleke nge-STD ngale khithi, njenge-Treponema pallidum, i-Neisseria gonorrhoeae, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium, njll., engaphandle kwebanga lokutholwa kwekhithi. |
Izinsimbi Ezisebenzayo | Ingakwazi ukufanisa amathuluzi we-PCR we-fluorescent avamile emakethe. I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |