I-Herpes Simplex Virus Type 1/2, (HSV1/2) Nucleic Acid

Isithombe Esifakiwe Se-Herpes Simplex Virus Type 1/2, (HSV1/2) Nucleic Acid

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola i-Herpes Simplex Virus Type 1 (HSV1) kanye ne-Herpes Simplex Virus Type 2 (HSV2) ngendlela esezingeni eliphezulu ngaphakathi kwe-vitro ukusiza ekuxilongeni nasekuphatheni iziguli ezinezifo ezisolwayo ze-HSV.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi yokuthola i-nucleic acid ye-HWTS-UR018A-Herpes simplex virus type 1/2, (HSV1/2) (i-Fluorescence PCR)

I-Epidemiology

Izifo ezithathelwana ngocansi (STD) ziseseyinye yezinsongo ezinkulu ekuphepheni kwempilo yomphakathi emhlabeni jikelele. Izifo ezinjalo zingaholela ekungazalini, ekuzalweni komntwana ngaphambi kwesikhathi, ekuvuvukeni kanye nezinkinga eziningi ezinkulu. Kunezinhlobo eziningi zezifo ezithathelwana ngocansi, okuhlanganisa amagciwane, amagciwane, i-chlamydia, i-mycoplasma kanye nama-spirochetes, phakathi kwazo i-Neisseria gonorrhoeae, i-Mycoplasma genitalium, i-Chlamydia trachomatis, i-HSV1, i-HSV2, i-Mycoplasma hominis, kanye ne-Ureaplasma urealyticum ezivamile.

I-genital herpes yisifo esivamile esidluliselwa ngocansi esibangelwa yi-HSV2, esithathelwana kakhulu. Eminyakeni yamuva nje, ukwanda kwe-genital herpes kukhuphuke kakhulu, futhi ngenxa yokwanda kwemikhuba yocansi eyingozi, izinga lokutholakala kwe-HSV1 ku-genital herpes likhuphuke futhi kwabikwa ukuthi liphezulu ngamaphesenti angama-20-30%. Ukutheleleka kokuqala ngegciwane le-genital herpes ngokuvamile akubonakali ngaphandle kwezimpawu zomtholampilo ezisobala ngaphandle kwe-herpes yendawo esikhumbeni noma esikhumbeni seziguli ezimbalwa. Njengoba i-genital herpes ibonakala ngokukhishwa kwegciwane impilo yonke kanye nokuthambekela kokubuyela emuva, kubalulekile ukuhlola amagciwane ngokushesha okukhulu futhi kuvimbele ukudluliselwa kwawo.

Isiteshi

I-FAM	I-HSV1
CY5	I-HSV2
I-VIC(HEX)	Ukulawula Kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	Uketshezi: ≤-18℃ Ebumnyameni
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	ukukhishelwa komchamo, ukukhishelwa komlomo wesibeletho
Ct	≤38
CV	≤5.0%
I-LoD	Amakhophi angu-50/ukusabela
Ukucaciswa	Akukho ukuhlangana kabusha namanye amagciwane e-STD afana ne-Treponema pallidum, i-Chlamydia trachomatis, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, i-Mycoplasma genitalium, ne-Ureaplasma urealyticum.
Izinsimbi Ezisebenzayo	Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio^®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler^®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR

Ukugeleza Komsebenzi

I-reagent yokukhipha enconywayo: I-Macro & Micro-Test Sample Release Release Release Reagent (HWTS-3005-8). Ukukhipha kufanele kwenziwe ngokuqinile ngokwemiyalelo.

I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhipha kufanele kwenziwe ngokuqinile ngokwemiyalelo. Ivolumu yokususa enconyiwe kufanele ibe ngu-80 μL.

I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP315) yi-Tiangen Biotech(Beijing) Co.,Ltd. Ukukhipha kufanele kwenziwe ngokuqinile ngokwemiyalelo. Umthamo wokususa onconyiwe kufanele ube ngu-80 μL.

Okwedlule: Izinhlobo eziyi-14 ze-HPV Nucleic Acid Typing

Olandelayo: I-Mycoplasma Hominis Nucleic Acid

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