Uhlobo lwe-Herpes Simplex Virus 1/2, (HSV1 / 2) i-nucleic acid
Igama lomkhiqizo
I-HWTS-UR018A-HERPES Simplex Virus Virus 1/2, (HSV1 / 2) I-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Izifo ezithathelwana ngocansi (STD) zisenye yezinsongo ezinkulu ekuvikelekeni kwempilo yomphakathi yomphakathi. Izifo ezinjalo zingaholela ekungenisweni kokuzala, ukulethwa kwezingane ngaphambi kwesikhathi, isimila kanye nezinkinga ezahlukahlukene ezingathi sína. Kunezinhlobo eziningi zamagciwane e-STD, kufaka phakathi amagciwane, amagciwane, i-chlamydia, i-mycoplasma kanye ne-spirochetes, phakathi kwayo i-neisrochetium, i-hpcoplasma hominis, ne-ureaplasma uroutyticum ajwayelekile.
I-Genital Herpes yisifo esivamile esidluliswa ngokobulili esibangelwa yi-HSV2, esitheleleka kakhulu. Eminyakeni yamuva, izigameko ze-herpes zangasese zikhule kakhulu, futhi ngenxa yokwanda kokuziphatha kobulili okuyingozi, okutholayo kwe-HSV1 kuma-herpes akhuphukile futhi kubikwa ukuthi kuphakeme njengama-20% -30%. Ukutheleleka kokuqala ngegciwane le-herpes yasolwandle kuthule kakhulu ngaphandle kwezimpawu ezisobala zemitholampilo ngaphandle kwe-herpes yasendaweni eMucosa noma isikhumba seziguli ezimbalwa. Njengoba i-genital herpes ibonakala ngokuchithwa kwegciwane impilo yonke kanye nobuqili ekubuyeleni, kubalulekile ukubhekisa ama-pathogen ngokushesha futhi kuvimbe ukuhanjiswa kwayo.
Insungubezi
-Yenza | I-HSV1 |
Cy5 | HSV2 |
I-VIC (Hex) | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
Ukubeka | Uketshezi: ≤-18 ℃ ebumnyameni |
Ishalofu-impilo | Izinyanga eziyi-12 |
Uhlobo lwe-Specimen | Okwebiwe kwe-Urethral, Okethe Besibeletho |
Ct | ≤38 |
CV | ≤5.0% |
Indlu edayisa insimbi | Amakhophi / Ukuphendula |
Ukucacisa | Akukho ukuthuthukiswa kokuphindaphindwa kwamanye ama-std palidum afana ne-treponema pallidum, i-chlamydia trachomatis, i-neiscoplasma honinis, i-mycoplasma hominis, i-mycoplasma geninium, ne-ureeaplasma urealyticum. |
Izinsimbi ezisebenzayo | Ingaqondanisa amathuluzi we-PCR e-PCR eseMakethe emakethe. Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-reagent enconyelwe i-reagent: I-Macro & Micro-Test Sampula Sampula Represe Reagent (HWS-3005-8). Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalo.
I-reaglent enconyiwe ye-realent: I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3017-50, HWTS-3017-50, HWS-3017-48, HWS-3017-48, HWS-3017-46, HWS-3017-46, I-HWS-3017-96, (engasetshenziswa ngeMacro & Micro-Test I-automatic nucleic acid extractor (HWTS-3006C, HWS-3006B)) nguJiangsu I-Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalo. IVolumu Ye-Exteased Enconyiwe kufanele ibe ngu-80 μl.
I-realent enconyiwe ye-realent: Ukukhishwa kwe-nucleic acid noma i-realegent yokuhlanza (ypp15) ngeTiangen Biotech (Beijing) Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalo. IVolumu Ye-Exteased Enconyiwe kufanele ibe ngu-80 μl.