I-Herpes Simplex Virus Type 1/2,(HSV1/2) Nucleic Acid
Igama lomkhiqizo
HWTS-UR018A-Herpes simplex virus uhlobo 1/2, (HSV1/2) ikhithi yokuthola i-nucleic acid (i-Fluorescence PCR)
I-Epidemiology
Izifo ezithathelwana ngocansi (STD) zisengenye yezinsongo ezinkulu ekuvikelekeni kwezempilo yomphakathi emhlabeni jikelele.Izifo ezinjalo zingaholela ekungazali, ukubeletha kwengane ngaphambi kwesikhathi, isimila kanye nezinkinga ezihlukahlukene ezinzima.Kunezinhlobo eziningi zamagciwane e-STD, okuhlanganisa amagciwane, amagciwane, i-chlamydia, i-mycoplasma ne-spirochetes, phakathi kwazo i-Neisseria gonorrhoeae, i-Mycoplasma genitalium, i-Chlamydia trachomatis, i-HSV1, i-HSV2, i-Mycoplasma hominis, ne-Ureaplasma urealyticum evamile.
I-Genital herpes yisifo esithathelwana ngocansi esivamile esibangelwa i-HSV2, esithathelwanayo kakhulu.Eminyakeni yamuva, izigameko ze-herpes zangasese ziye zanda kakhulu, futhi ngenxa yokwanda kokuziphatha kocansi okuyingozi, izinga lokutholwa kwe-HSV1 ku-herpes yobulili liye landa futhi kubikwa ukuthi liphezulu njenge-20% -30%.Ukutheleleka kokuqala ngegciwane le-herpes yangasese ngokuvamile kuthule ngaphandle kwezimpawu zomtholampilo ezisobala ngaphandle kwe-herpes yendawo ku-mucosa noma isikhumba seziguli ezimbalwa.Njengoba i-herpes yangasese ibonakala ngokuchithwa kwegciwane impilo yonke kanye nokuthambekela ekubuyeleni kabusha, kubalulekile ukuhlola amagciwane ngokushesha ngangokunokwenzeka futhi uvimbele ukudluliswa kwawo.
Isiteshi
FAM | HSV1 |
CY5 | HSV2 |
I-VIC(HEX) | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
Isitoreji | Uketshezi: ≤-18℃ ebumnyameni |
Impilo yeshelufu | Izinyanga ezingu-12 |
Uhlobo lwesifanekiso | ukukhishwa kwe-urethra, ukukhishwa komlomo wesibeletho |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Amakhophi/ukusabela |
Ukucaciswa | Akukho ukuhlangana kabusha namanye amagciwane e-STD afana ne-Treponema pallidum, i-Chlamydia trachomatis, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, i-Mycoplasma genitalium, ne-Ureaplasma urealyticum. |
Izinsimbi Ezisebenzayo | Ingakwazi ukufanisa amathuluzi we-PCR we-fluorescent avamile emakethe. I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Sample Release Reagent(HWTS-3005-8).Ukukhipha kufanele kwenziwe ngokuqinile ngokuvumelana nemiyalelo.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokwemiyalelo.Ivolumu ye-lution enconyiwe kufanele ibe ngu-80 μL.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: Isizinda se-Nucleic Acid noma Isisetshenziswa Sokuhlanza(YDP315) yi-Tiangen Biotech(Beijing) Co.,Ltd.Ukukhipha kufanele kwenziwe ngokuqinile ngokuvumelana nemiyalelo.Ivolumu ye-lution enconyiwe kufanele ibe ngu-80 μL.