I-Herpes Simplex Virus Type 2 Nucleic Acid

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwekhwalithi ye-herpes simplex virus uhlobo 2 lwe-nucleic acid ku-swab ye-urethral yabesilisa kanye namasampula womlomo wesibeletho wesifazane.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama Lomkhiqizo

HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)

Ukusetshenziswa Okuhlosiwe

Le kit isetshenziselwa ukutholwa kwekhwalithi ye-herpes simplex virus uhlobo 2 lwe-nucleic acid ku-swab ye-urethral yabesilisa kanye namasampula womlomo wesibeletho wesifazane.

I-Epidemiology

I-Herpes Simplex Virus Type 2 (HSV2) igciwane eliyindilinga elihlanganiswe ne-tegument, capsid, core, nemvilophu, futhi liqukethe i-DNA yomugqa enemicu ekabili.I-Herpes virus ingangena emzimbeni ngokuthintana okuqondile noma ukuthintana ngokocansi nesikhumba kanye nolwelwesi lwamafinyila, futhi ihlukaniswe yaba okuyinhloko nokuphindaphindayo.Ukutheleleka emgudwini wokuzala kubangelwa ikakhulukazi i-HSV2, iziguli zesilisa zibonakala njengezilonda ze-penile, futhi iziguli zesifazane zibonakala njengezilonda zomlomo wesibeletho, i-vulvar, ne-vaginal.Izifo zokuqala zegciwane le-herpes ezithweni zangasese ngokuvamile ziyizifo eziphindaphindayo, ngaphandle kwe-herpes embalwa yendawo enolwelwesi lwamafinyila noma isikhumba, iningi lazo elingenazo izimpawu zomtholampilo ezisobala.Ukutheleleka kwe-herpes yangasese kunezici zokuthwala igciwane impilo yonke kanye nokuphindaphinda kalula, futhi kokubili iziguli nabathwali bawumthombo wokutheleleka kwalesi sifo.E-China, izinga le-serological positive le-HSV2 licishe libe ngu-10.80% kuya ku-23.56%.Isigaba sokutheleleka nge-HSV2 singahlukaniswa sibe ukutheleleka okuyisisekelo kanye nokutheleleka okuphindaphindayo, futhi cishe u-60% weziguli ezithelelekile ze-HSV2 ziyaphinda.

I-Epidemiology

I-FAM: I-Herpes Simplex Virus Type 2 (HSV2)·

I-VIC(HEX): Ukulawula Kwangaphakathi

Izilungiselelo ze-PCR Amplification Conditions

Isinyathelo	Imijikelezo	Izinga lokushisa	Isikhathi	QoqaFi-luorescentSama-ignalsnoma cha
1	1 Umjikelezo	50℃	5 imiz	No
2	1 Umjikelezo	95℃	10 imiz	No
3	40 imijikelezo	95℃	15 amasekhondi	No
4	40 imijikelezo	58℃	31 isekhondi	Yebo

Imingcele Yezobuchwepheshe

Isitoreji
Uketshezi	≤-18℃ ebumnyameni
Impilo yeshelufu	Izinyanga ezingu-12
Uhlobo lwesifanekiso	I-swab yomlomo wesibeletho wesifazane, I-swab ye-urethral yesilisa
Ct	≤38
CV	≤5.0%
LoD	50Amakhophi/ukusabela
Ukucaciswa	Akukho ukuhlangana kabusha namanye amagciwane e-STD, njenge-Treponema pallidum, i-Chlamydia trachomatis, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium nokunye.
Izinsimbi Ezisebenzayo	Ingakwazi ukufanisa amathuluzi we-PCR we-fluorescent avamile emakethe. I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System.