I-Herpes Simplex Virus Virus 2 Uhlobo lwe-nucleic acid
Igama lomkhiqizo
I-HWTS-UR007A-HERPES Simplex Virus Virus Virus 2 I-Nucleic Acid Detection Kit (Fluorescence PCR)
Ukusetshenziswa okuhlosiwe
Le kit isetshenziselwa ukutholwa kwe-herpes Simplex Virus Virus 2 uhlobo lwe-nucleic e-urethral urethral swab kanye namasampula wesibeletho sowesifazane.
I-Epidemiology
I-Herpes Simplempx Virus Virus 2 (HSV2) iyinhloso eyindilinga eyenziwe nge-tegument, i-capsid, umnyombo kanye nemvilophu, futhi iqukethe i-DNA ehlanganisiwe ebilisiwe. Igciwane le-Herpes lingangena emzimbeni ngokuxhumana ngqo noma ukuxhumana ngokocansi ngesikhumba kanye nolwelwesi lwama-mucous, futhi lihlukaniswe ngamabanga aphansi futhi ajwayelekile. Ukutheleleka kwe-Fourctupitive kudalwa yi-HSV2, iziguli zabesilisa zibonakaliswa njengezilonda ze-penile, futhi iziguli zabesifazane zibonakaliswa njengomlomo wesibeletho, i-vulvar, kanye nezilonda zofuba. Ukutheleleka kokuqala kwegciwane le-genital herpes yizifo eziphindaphindwayo, ngaphandle kwe-herpes yendawo embalwa enolwelwesi lwe-mucous noma isikhumba, iningi lazo elingenazo izimpawu zomtholampilo ezisobala. Ukutheleleka kwe-genital Herpes kunezimpawu zokuthwala igciwane lempilo yonke nokuphindaphinda okulula, futhi zombili iziguli nabathwali bangumthombo wokutheleleka kwalesi sifo. EChina, inani elifanele lezenkolo le-HSV2 licishe libe ngu-10.80% laya ku-23.56%. Isiteji sokutheleleka kwe-HSV2 singahlukaniswa sibe ukutheleleka okuyisisekelo nokutheleleka okujwayelekile, futhi cishe ama-60% eziguli ze-HSV2 ezinegciwane zilahla.
I-Epidemiology
Fam: Herpes Simplex Virus hlobo 2 (HSV2) ·
I-VIC (Hex): Ukulawulwa Kwangaphakathi
Izimo ze-PCR zokumenyezelwa
Gxatha | Imijikelezo | Izinga lokushisa nokubanda | Isikhathi | ButhaFI-LuorescentSukunganakinoma cha |
1 | 1 Umjikelezo | 50 ℃ | 5mins | No |
2 | 1 Umjikelezo | 95 ℃ | 10mins | No |
3 | Imijikelezo engama-40 | 95 ℃ | I-15SECS | No |
4 | 58 ℃ | I-31Secs | Yebo |
Amapharamitha Ezobuchwepheshe
Ukubeka | |
Uketshezi | ≤-18 ℃ ebumnyameni |
Ishalofu-impilo | Izinyanga eziyi-12 |
Uhlobo lwe-Specimen | I-Swab yesibeletho yabesifazane, i-urethral yabesilisa |
Ct | ≤38 |
CV | ≤5.0% |
Indlu edayisa insimbi | Ama-50copies / ukuphendula |
Ukucacisa | Akukho okuphambana nokuphindaphindeka namanye ama-STD pathogen, afana ne-treponema pallidum, i-chlamydia trachomatis, i-ureaplasma urealyticum, Mycoplasma ureatis, MyCoplasma geninis, MyCoplasma Genitalium kanye njll. |
Izinsimbi ezisebenzayo | Ingaqondanisa amathuluzi we-PCR e-PCR eseMakethe emakethe. Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR I-Biorad CFX Opus 96 uhlelo lwe-real-time PCR. |