Mycoplasma hominis nucleic acid
Igama lomkhiqizo
I-HWTS-UR004A-MICCOPLASMA KITINISIIS NUCLEIC Acid Detection Detection (Fluorescence PCR)
I-Epidemiology
Izifo ezithathelwana ngocansi (ama-STD) zisenye yezinsongo ezibalulekile ekuvikelekeni kwempilo yomphakathi yomphakathi emhlabeni, okungaholela ekutheni i-infertility, ukuzalwa kwengane ngaphambi kwesikhathi, izitho zaphakade kanye nezinkinga ezahlukahlukene. I-MyCoplasma Hominis ikhona ephepheni le-genitorilary futhi lingadala izimpendulo zokuvuvukala epheshana elinazokwelapha. Ukutheleleka kwe-MH ye-Genitourioun Tract kungadala ukuthi izifo ezinjenge-urethritis engeyona eye-gonococcal, i-epidedimitis, njll. Ngasikhathi sinye, inhlekelele ejwayelekile yokutheleleka kwe-MH i-salpititis, futhi inani elincane leziguli lingaba ne-endometritis kanye nesifo sokuvuvukala kwe-pelvic.
Insungubezi
-Yenza | Ithagethi ye-MH |
I-VIC (Hex) | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
Ukubeka | Uketshezi: ≤-18 ℃ ebumnyameni |
Ishalofu-impilo | Izinyanga eziyi-12 |
Uhlobo lwe-Specimen | Okwebiwe kwe-Urethral, Okethe Besibeletho |
Ct | ≤38 |
CV | <5.0% |
Indlu edayisa insimbi | 1000copies / ml |
Ukucacisa | Akukho okuphambana nokuphindaphindeka namanye ama-pathogens athelelekayo e-STD, angaphandle kwebanga lokutholwa, futhi akukho run-receatytis, i-ypeaplasma ureartticum, i-hercoplasma urorrhoeae, i-herpees Simplex Virus Virus hlobo 2 , njll. |
Izinsimbi ezisebenzayo | Ingaqondanisa amathuluzi we-PCR e-PCR eseMakethe emakethe. Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
I-reagent enconyelwe i-reagent: I-Macro & Micro-Test Sampula Sampula Represe Reagent (HWS-3005-8). Ukukhishwa kufanele kwenziwe ngokuqinile nemiyalo.
Inketho 2.
I-reaglent enconyiwe ye-realent: I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3017-50, HWTS-3017-50, HWS-3017-48, HWS-3017-48, HWS-3017-46, HWS-3017-46, I-HWS-3017-96, (engasetshenziswa ngeMacro & Micro-Test I-automatic nucleic acid extractor (HWTS-3006C, HWS-3006B)) nguJiangsu I-Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngemiyalo. IVolumu Ye-Exteased Enconyiwe kufanele ibe ngu-80 μl.
Inketho 3.
I-realent enconyiwe ye-realent: Ukukhishwa kwe-nucleic acid noma i-realegent yokuhlanza (ydp302) nguTiangen Biotech (Beijing) Co, Ltd .. Ukukhishwa kufanele kwenziwe ngokuqinile nemiyalo kanye nevolumu yokucishwa okunconyiwe kungu-80μL.