I-Mycoplasma Hominis Nucleic Acid
Igama lomkhiqizo
I-HWTS-UR004A-Mycoplasma Hominis Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Izifo ezithathelwana ngocansi (STD) zisengenye yezinsongo ezibalulekile ekuvikelekeni kwezempilo yomphakathi emhlabeni wonke, ezingaholela ekungazali, ukuzalwa kombungu ngaphambi kwesikhathi, i-tumorigenesis kanye nezinkinga ezinzima ezihlukahlukene.I-Mycoplasma hominis ikhona emgudwini we-genitourinary futhi ingabangela izimpendulo zokuvuvukala ku-genitourinary tract.Ukutheleleka kwe-MH kwe-genitourinary tract kungabangela izifo ezifana ne-non-gonococcal urethritis, i-epididymitis, njll, naphakathi kwabesifazane, okungabangela ukuvuvukala kwesistimu yokuzala esakazeka egxile emlonyeni wesibeletho.Ngesikhathi esifanayo, inkinga evamile yokutheleleka kwe-MH i-salpingitis, futhi inani elincane leziguli lingase libe ne-endometritis nesifo sokuvuvukala kwe-pelvic.
Isiteshi
FAM | Ithagethi ye-MH |
I-VIC(HEX) | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
Isitoreji | Uketshezi: ≤-18℃ ebumnyameni |
Impilo yeshelufu | Izinyanga ezingu-12 |
Uhlobo lwesifanekiso | ukukhishwa kwe-urethra, ukukhishwa komlomo wesibeletho |
Ct | ≤38 |
CV | <5.0% |
LoD | 1000Amakhophi/mL |
Ukucaciswa | Akukho ukuhlangana kabusha namanye amagciwane okutheleleka nge-STD, angaphandle kwebanga lokutholwa, futhi akukho ukuhlangana kabusha kwe-chlamydia trachomatis, ureaplasma urealyticum, neisseria gonorrhoeae, mycoplasma genitalium, herpes simplex virus hlobo 1, herpes simplex virus uhlobo 2 , njll. |
Izinsimbi Ezisebenzayo | Ingakwazi ukufanisa amathuluzi we-PCR we-fluorescent avamile emakethe. Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ukukhishwa kufanele kwenziwe ngokuhambisana ngokuqinile nemiyalelo.
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokwemiyalelo.Ivolumu ye-lution enconyiwe kufanele ibe ngu-80 μL.
Inketho yesi-3.
Isisetshenziswa esisebenzayo sokumba esinconyiwe: I-Nucleic Acid Extraction noma I-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co., Ltd. Ukukhipha kufanele kwenziwe ngokuhambisana nemiyalelo ngokuqinile futhi nevolumu yokuhlaziya enconyiwe ngu-80µL.