I-Mycoplasma Pneumoniae (MP)
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-RT024 Mycoplasma Pneumoniae（MP） Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Mycoplasma pneumoniae (MP) uhlobo lwe-microorganism encane kakhulu ye-prokaryotic, ephakathi kwamagciwane negciwane, enesakhiwo samaseli kodwa engenalo udonga lwamaseli. I-MP ibangela kakhulu ukutheleleka kwendlela yokuphefumula yabantu, ikakhulukazi ezinganeni nasebancane. Ingabangela i-mycoplasma pneumonia yabantu, ukutheleleka kwendlela yokuphefumula yezingane kanye ne-pneumonia engavamile. Izimpawu zemitholampilo ziyahlukahluka, eziningi zazo ukukhwehlela okukhulu, umkhuhlane, ukubanda, ikhanda elibuhlungu, umphimbo obuhlungu. Ukutheleleka kwendlela yokuphefumula ephezulu kanye ne-bronchial pneumonia yizona ezivame kakhulu. Ezinye iziguli zingathuthuka kusukela ekuthelelekeni kwendlela yokuphefumula ephezulu kuya kwi-pneumonia enzima, ukucindezeleka okukhulu kokuphefumula nokufa kungase kwenzeke.
Isiteshi
| I-FAM | I-Mycoplasma pneumoniae |
| I-VIC/HEX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-sputum, i-oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-200/mL |
| Ukucaciswa | a） Ukusabela okuphambene: akukho ukusabela okuphambene ne-Ureaplasma urealyticum, i-Mycoplasma genitalium, i-Mycoplasma hominis, i-Streptococcus pneumoniae, i-Chlamydia pneumoniae, i-Haemophilus influenzae, i-Klebsiella pneumoniae, i-Staphylococcus aureus, i-Mycobacterium tuberculosis, i-Legionella pneumophila, i-Pseudomonas aeruginosa, i-Acinetobacter baumannii, igciwane le-Influenza A, igciwane le-Influenza B, igciwane le-Parainfluenza uhlobo lwe-I/II/III/IV, i-Rhinovirus, i-Adenovirus, i-Human metapneumovirus, igciwane le-Respiratory syncytial kanye ne-human genomic nucleic acid. b）Ikhono lokulwa nokuphazamiseka: akukho ukuphazamiseka lapho izinto eziphazamisayo zihlolwa ngamazinga alandelayo: i-hemoglobin (50mg/L), i-bilirubin (20mg/dL), i-mucin (60mg/mL), igazi lomuntu elingu-10% (v/v), i-levofloxacin (10μg/mL), i-moxifloxacin (0.1g/L), i-gemifloxacin (80μg/mL), i-azithromycin (1mg/mL), i-clarithromycin (125μg/mL), i-erythromycin (0.5g/L), i-doxycycline (50mg/L), i-minocycline (0.1g/L). |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
(1) Isampula Yesikhwehlela
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Engeza u-200µL we-saline evamile ku-precipitate ecutshungulwayo. Ukukhipha okulandelayo kufanele kwenziwe ngokwemiyalelo yokusebenzisa. Ivolumu yokususa enconyiwe ingu-80µL. I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R). Ukukhipha kufanele kwenziwe ngokuqinile ngokwemiyalelo yokusebenzisa. Ivolumu yokususa enconyiwe ingu-60µL.
(2) I-Oropharyngeal swab
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhipha kufanele kwenziwe ngokwemiyalelo yokusebenzisa. Umthamo wokukhipha onconyiwe wesampula ungama-200µL, kanti umthamo wokususa onconyiwe ungama-80µL. I-reagent yokukhipha enconyiwe: I-QIAamp Viral RNA Mini Kit (52904) noma i-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R). Ukukhipha kufanele kwenziwe ngokuqinile ngokwemiyalelo yokusebenzisa. Umthamo wokukhipha isampula onconywayo ngu-140µL, kanti umthamo wokukhipha onconywayo ngu-60µL.
