I-MyCoplasma Pneumoniae (MP)
Igama lomkhiqizo
I-HWTS-RT024 MITCOPLASMA PNEUMONIAE (MP) I-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-MyCoplasma Pneumoniae (MP) uhlobo lwe-microorganism encane kunazo zonke, oluphakathi kwamagciwane negciwane, elinesakhiwo seseli kepha alukho udonga lweseli. I-MP ikakhulukazi ibangela ukutheleleka nge-Humal Resirandery Courctiratory, ikakhulukazi ezinganeni nakubantu abasha. Kungadala ukuthi i-MyCoplasma Pneumonia ye-Mycoplasma, izifo zezingane zokuphefumula kanye ne-atypical pneumonia. Ukubonakaliswa kwemitholampilo kuyinto ehlukahlukene, iningi lazo elikhwehlela elinzima, umkhuhlane, amakhaza, ikhanda, umphimbo obuhlungu. Ukutheleleka okuphezulu kokuphefumula okuphezulu kanye ne-bronchial pneumonia yiyona evame kakhulu. Ezinye iziguli zingakhula kusuka ekuthelelekeni kwe-Upper Respiratory Tract kuya ku-pneumonia enzima, ukucindezelwa okukhulu kokuphefumula nokufa kungenzeka.
Insungubezi
| -Yenza | I-MyCoplasma Pneumoniae |
| VIC / Hex | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Sputum, OropharyNeal SwapS |
| Ct | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | Amakhophi angama-200 / ml |
| Ukucacisa | a) Ukuvuselelwa kabusha kwemvume: Akukho ukubhekisisa ukushesha nge-ureaplasma urealyticum, Mycoplasma geniitalium, i-stcoplasma hominis, i-strepplias pneumonia, i-haemophilus informan I-Leginonollas Pneumophila, i-Pseudomonas Aeruginosa, umkhuhlane wegciwane, umkhuhlane wegciwane, uhlobo lwe-adenoviruenze b) Ikhono lokulwa ne-Anti-Sument Igazi lomuntu, i-levofloxacin (10μg / ml), iMoxifloxacin (0.1G / L), Gemifloxacin . |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Plus real-time PCCRECT SYPELY (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
(1) Isampula yeSputum
I-reaglent enconyiwe ye-realent: I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3017-50, HWTS-3017-50, HWS-3017-48, HWS-3017-48, HWS-3017-46, HWS-3017-46, I-HWS-3017-96, (engasetshenziswa ngeMacro & Micro-Test I-automatic nucleic acid extractor (HWTS-3006C, HWS-3006B)) nguJiangsu I-Macro & Micro-Test Med-Tech Co, Ltd. engeza i-200μL ye-saline ejwayelekile ku-precipitate esetshenzisiwe. Ukukhishwa okulandelayo kufanele kwenziwe ngokuya ngomyalo wokusetshenziswa. IVolumu Echithiwe Enconyiwe yi-realent ye-80μL.ecommid.Revid Reagent: I-Nucleic Acid Extraction noma i-Reagent yokuhlanza (ypp15-R). Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngomyalo wokusebenzisa. IVolumu Ye-Expery Enconyiwe ingu-60μL.
(2) I-Oropharyngengeal Swab
I-reaglent enconyiwe ye-realent: I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3017-50, HWTS-3017-50, HWS-3017-48, HWS-3017-48, HWS-3017-46, HWS-3017-46, I-HWS-3017-96, (engasetshenziswa ngeMacro & Micro-Test I-automatic nucleic acid extractor (HWTS-3006C, HWS-3006B)) nguJiangsu I-Macro & Micro-Test Med-Tech Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuya ngomyalo wokusetshenziswa. Ivolumu yokukhishwa enconyiwe yesampula i-200μL, futhi ivolumu yokuqedwa okunconyiwe liyi-realent ye-80μL.Reeced.Recoment RNA MINI KIT (52904) noma i-reapent ye-nucpleic acid (ypp15-r). Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngomyalo wokusebenzisa. Ivolumu yokukhishwa enconyiwe yesampula yi-140μL, futhi ivolumu yokuqedwa elinconyiwe lingu-60μL.
