I-Clostridium difficile toxin A/B gene (C.diff)
Igama lomkhiqizo
I-HWTS-OT031A Nucleic Acid Detection Kit ye-Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Clostridium difficile (CD), i-gram-positive anaerobic sporogenic Clostridium difficile, ingenye yezifo eziyinhloko ezibangela izifo zamathumbu e-nosocomial. Ngokomtholampilo, cishe i-15% ~ 25% yohudo oluhlobene ne-antimicrobial, 50% ~ 75% ye-colitis ehlobene ne-antimicrobial kanye ne-95% ~ 100% ye-pseudomembranous enteritis ibangelwa ukutheleleka kwe-Clostridium difficile (CDI). I-Clostridium difficile iyi-pathogen enemibandela, ehlanganisa izinhlobo ze-toxigenic kanye nezinhlobo ezingenayo i-toxigenic.
Isiteshi
| FAM | tcdAisakhi sofuzo |
| I-ROX | tcdBisakhi sofuzo |
| VIC/HEX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | indle |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Ukucaciswa | sebenzisa le kit ukuthola amanye amagciwane amathumbu afana ne-Escherichia coli, i-Staphylococcus aureus, i-Shigella, i-Salmonella, i-Vibrio parahaemolyticus, i-Group B Streptococcus, i-Clostridium difficile non-pathogenic strains, i-Adenovirus, i-rotavirus, i-norovirus, i-influenza ye-DNA, imiphumela ye-influenza yabantu zonke negative. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System(I-FQD-96A,I-HangzhouUbuchwepheshe be-Bioer) I-MA-6000 ye-Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Engeza u-180μL webuffer ye-lysozyme ku-precipitate (nciphisa i-lysozyme ibe ngu-20mg/mL nge-lysozyme diluent), i-pipette ukuze uxube kahle, futhi usebenze ku-37°C ngaphezu kwemizuzu engu-30. Thatha u-1.5mL we-RNase/DNase-free centrifuge tube, futhi18I-0μL yokulawula okuhle nokulawula okungalungile ngokulandelana. Engeza10U-μL wokulawula kwangaphakathi kusampula ezohlolwa, ukulawula okuhle, nokulawula okungekuhle ngokulandelana, futhi usebenzise i-Nucleic Acid Extraction noma Purification Reagent (YDP302) ye-Tiangen Biotech (Beijing) Co., Ltd. ukuze uthole isampula ye-DNA ekhishwe, futhi sicela ulandele ngokuqinile imiyalelo yokusebenzisa izinyathelo ezithile. Sebenzisa i-DNase/RNase mahhala i-H2O ye-lution, futhi ivolumu ye-lution enconyiwe ingu-100μL.
Inketho yesi-2.
Thatha u-1.5mL we-RNase/DNase-free centrifuge tube, bese wengeza u-200μL wokulawula okuhle nokulawula okungalungile ngokulandelana. Engeza10I-μL yokulawula kwangaphakathi kusampula okufanele ihlolwe, ukulawula okuhle, nokulawula okungekuhle ngokulandelana, futhi kusetshenziswe i-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid (0HTS-Extramatic Nucleic Acid (0HTS-6W). Ukukhishwa kufanele kwenziwe ngokuhambisana ngokuqinile nomyalelo wokusetshenziswa, futhi umthamo we-lution onconyiwe ngu-80μL.
