I-Closerpidium Efficile Toxin A / B Gene (C.DIFF)
Igama lomkhiqizo
I-HWTS-OT031A NUCLEIC Acid Detection Kit for Closerpidium Fricile Toxin A / B Gene (C.DIFF) (Fleorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Closerdidium Efficile (CD), i-anaerogic enhle ye-anaerogenic sporogenic sporogenic sporogenic ye-anaicial, kungenye yamagciwane amakhulu adala ukutheleleka kwamathumbu aseNosocomial. Ngokwenyama, cishe ngo-15% ~ 25% wohudo oluhlobene nokudlalwa, 50% ~ 75% we-colitis ehlobene ne-colitis ahlobene nama-95% ~ 100% we-pseudomembranous enteritis kubangelwa ukutheleleka ngengozi (CDI). I-Closerdidium Efficile iyi-pathogen enemibandela, kufaka phakathi ama-toxigenic perkin kanye nezinhlobo ezingezona ezobuthi.
Insungubezi
| -Yenza | I-TCDAuhlobo |
| Ivupho | tcdbuhlobo |
| VIC / Hex | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | isihlalo esinyoneni |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | I-200cFU / ml |
| Ukucacisa | Sebenzisa leli khithi ukuthola amanye amagciwane amathumbu anjenge-Escherichia Coli, Staphylococcus aureus, Shigella, SALAVOMILEEEMOLYTICUS, i-pubrio parahaemolyticus, i-adenovirus, i-tloonselza, i-norovirus, umkhuhlane virus, umkhuhlane b virus kanye negciwane lomuntu genomic I-DNA, imiphumela ilungile. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 kanye nohlelo lwe-Real-time PCCRECCEED SYPEL (I-FQD-96A,I-hangzhouUbuchwepheshe be-bioer) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
Faka i-180μL ye-lysozyme buffer ku-precipitate (Dlulisa i-lysozyme kuya ku-20mg / ml nge-lysozyme diluent), i-pipette ukuxuba kahle, bese ucubungula ku-37 ° C imizuzu ye-Rnase / Dnase-free centrifuuge Tube, bese ufaka18I-05L yokulawulwa okuhle nokulawulwa okungalungile ngokulandelana. Enezelela10I-μL yokulawulwa kwangaphakathi kwisampula ukuze ihlolwe, ukulawula okuhle, nokulawulwa okungalungile ngokulandelana, futhi usebenzise i-Nucleic Acid Extraction noma i-Reajing Reagent (ydp302) yiTiangen Biotech (Beijing) Co, Ltd. Sicela ulandele ngokuqinile imiyalo yokusetshenziswa kwezinyathelo ezithile. Sebenzisa i-dnase / rnase free h2O Ngokwengxenye, futhi ivolumu yokuqedwa enconyiwe liyi-100μL.
Inketho 2.
Thatha u-1.5ml we-tube ye-RNASE / DNASE-free centrifuge, bese wengeza i-200μL yokulawulwa okuhle nokulawulwa okungalungile ngokulandelana. Enezelela10μl yokulawulwa kwangaphakathi kwisampula ukuze ihlolwe, ukulawula okuhle, nokulawulwa okungalungile ngokulandelana, futhi usebenzise i-Macro & RNA Toust Viral DNA / RNA KIT (HWS-3004-32, HWS-3004- 96) kanye ne-macro & micro-test automatic extractor ye-nucleic acid (HWS-3006). Ukukhishwa kufanele kwenziwe ngokuqinile ngokuhambisana nokufundiswa okusetshenziselwa, futhi ivolumu yokuqedwa enconyiwe yi-80μL.
